The following compounds were obtained from Sigma: CHX (100 mg/mL stock in DMSO; C1988), PURO (10 mg/mL stock in water; P9620), ANS (10 mg/mL stock in DMSO; A5862), EME (100 mg/mL stock in water; E2375), and HHT (10 mg/mL stock in DMSO; SML1091). LTM (5 mM stock in DMSO; 506291) was obtained from Millipore. BVD (10 mM stock in DMSO) and DIDB (10 mM in DMSO) were kind gifts from Tin Tin Su (University of Colorado at Boulder) and Malia Potts (St. Jude Children's Research Hospital), respectively. NSC119893 (50 mM stock in DMSO) was obtained from the National Cancer Institute Development Therapeutics Program.
Sml1091
Manufactured by Merck Group
The SML1091 is a laboratory equipment product manufactured by the Merck Group. It is designed for general laboratory use. The core function of the SML1091 is to provide a reliable and consistent performance in various laboratory applications. No further details are available without the risk of extrapolation or interpretation.
Automatically generated - may contain errors
2 protocols using sml1091
1
Sourcing and Handling Chemical Compounds
2
Growth and Stress Induction in Haloferax
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ΔhdrB) (Hv) single colonies were picked and grown overnight at 42°C with shaking at 220 rpm (Amerex Gyromax 737) in Hv-YPC medium supplemented with thymidine (50 μg/mL final concentration) (21) until saturation (OD600 > 1.0). These cultures were then diluted to OD600 0.02 in fresh media, grown to OD600 0.4 and split evenly into two flasks; one flask was used as a notreatment control and the other was treated with either 1 mg/mL homo-harringtonine (HHT, Sigma Catalog #SML1091), 20 mM serine hydroxamate (SHX, Sigma Catalog #S4503), or 100 μg/mL anisomycin (ANS, Sigma Catalog #A9789) with final concentrations as indicated in the text. Cells were harvested by centrifugation, filtering, or direct freezing of the culture in liquid nitrogen.
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