Ldh release quantification cytotoxicity assay kit

Cells were inoculated into the 96-well cell culture plate. Measurement of LDH release was conducted using the LDH release quantification cytotoxicity Assay Kit (Beyotime, Shanghai, China) as per the manufacturer’s instructions. To measure the caspase-1 activity, the provided instructions from Beyotime (Shanghai, China) were followed. PNA in the kit was diluted to create a range of standard solutions with different concentrations. A determination system was prepared by mixing the diluted PNA with the lysed cell suspension in a 1:9 ratio. A standard curve was generated using the prepared standard solutions. Following the kit instructions, a buffer system was prepared, and the absorbance at 405 nm was measured using an enzyme reader.

LDH release was measured using the LDH release quantification cytotoxicity Assay Kit (Beyotime Biotechnology; C0016) in accordance with the manufacturer’s instructions. Caspase-1 activity was measured using a caspase-1 activity assay kit (Beyotime Biotechnology; C1101) based on the cleavage of substrate YVAD-AFC, according to the manufacturer’s instructions.