G1432

Manufactured by Solarbio

Sourced in China

The G1432 is a laboratory equipment designed for the detection and measurement of fluorescence signals. It is a highly sensitive instrument that can be used for various applications in the field of molecular biology, biochemistry, and cell biology.

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Lab products found in correlation

G1120 for h e, by Solarbio (1 mentions) G1120, by Solarbio (1 mentions) Inverted microscope, by Olympus (1 mentions) Ht7700, by Hitachi (1 mentions)

4 protocols using g1432

Histological Analysis of Mouse Olfactory Bulbs

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The mouse olfactory bulbs were fixed in 4% PFA solution, embedded in paraffin, and then cut into 5 μm slices. The slices were stained with HE and NISSL according to the manual (G1120 for HE and G1432 for NISSL; Solarbio, Beijing, China).

Cai C., Luo Q., Jia L., Xia Y., Lan X., Wei X., Shi S., Liu Y., Wang Y., Xiong Z., Shi R., Huang C, & Chen Z. (2023). TRIM67 Implicates in Regulating the Homeostasis and Synaptic Development of Mitral Cells in the Olfactory Bulb. International Journal of Molecular Sciences, 24(17), 13439.

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Histological Analysis of Rat Brain

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At 7 days after HI, the rats were deeply anesthetized and the chest cavity was quickly opened to expose the heart. Then, 20 mL normal saline and 20 mL 4% PFA solution were used for cardiac perfusion at a rate of 10–15 mL/min until their livers were cleared of blood. The rat brains were freshly extracted after decapitation, and then the brain tissues were immediately fixed in 4% PFA for 24 h. After gradient dehydration using ethanol and xylene, the brain tissues were embedded in paraffin and cut into 5 μm thick sections, which were used for subsequent hematoxylin-eosin (HE) (G1120, Solarbio, Beijing, China) and Nissl (G1432, Solarbio, Beijing, China) staining according to the manufacturer’s instructions (Zhu J. J et al., 2021 (link)). The images of bright field were obtained under the optical microscope (Nikon Corporatin, Tokyo, Japan), and the number of neurons per mm³ was analyzed using Image J software (National Institutes of Health, Bethesda, MD, United States). In addition, 4 images per sample were quantitated (n = 4 rats per group).

Chen T., Hu Y., Lu L., Zhao Q., Tao X., Ding B., Chen S., Zhu J., Guo X, & Lin Z. (2023). Myricetin attenuates hypoxic-ischemic brain damage in neonatal rats via NRF2 signaling pathway. Frontiers in Pharmacology, 14, 1134464.

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Paraffin-Embedded Brain Tissue Sectioning and Nissl Staining

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The brains were fixed in a 4% paraformaldehyde solution and embedded in paraffin. The 5 μm sections were mounted on slides and stained with NISSL according to the manufacturer’s instructions (G1432, Solarbio, Beijing, China). The images were captured using an inverted microscope (Olympus, Tokyo, Japan).

Jia L., Chen Z., Pan T., Xia Y., He J., Jahangir A., Wei X., Liu W., Shi R., Huang C, & Luo Q. (2022). TRIM67 Deficiency Exacerbates Hypothalamic Inflammation and Fat Accumulation in Obese Mice. International Journal of Molecular Sciences, 23(16), 9438.

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Quantifying Neuronal Survival in Rat Brains

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First, the rats were perfused into the heart with 250mL of PBS followed by 400mL of 4% paraformaldehyde in PBS. Then, brain tissues were fixed again in 4% paraformaldehyde for 12h and dehydrated with 15 and 30% sucrose. Finally, brain tissues were embedded in optimal cutting temperature (OCT) compound (4583, SAKURA, USA) or paraffin and cut into 10-20µm-thick slices. H&E staining was stained using hematoxylin and eosin. For Nissl staining, paraffin-embedded slices were covered with 0.2% Nissl staining solution (G1432, Solarbio, China) for 10min. Then, slices were sealed and observed by light microscopy (CI-S, Nikon, Japan). The percentages of surviving neurons in right hemisphere/left hemisphere, in cortex/mm 2 and in cornu ammonis 1 (CA1)/mm were quantified using ImageJ software.
In electron microscopic observation, after fixed with 3% glutaraldehyde, brain tissues were incubated with 2% OsO4 and dehydrated in ethanol. Brain tissues were then dried, uranium lead double staining, and observed using transmission electron microscopy (hitachi, HT7700).

Tang B., Li Y., Xu X., Du G, & Wang H. (2024). Electroacupuncture Ameliorates Neuronal Injury by NLRP3/ASC/Caspase-1 Mediated Pyroptosis in Cerebral Ischemia-Reperfusion. Molecular neurobiology, 61(4).

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