LNCaP C4-2, LNCaP C4-2 MDV3100r, PC-3 cells were seeded at a density of 5 × 103 cells/well in 96-well plates with 100 μl culture medium containing 10% FBS and increasing concentration (0, 10, 25, 50, 75, 100 μg/ml) of GRT for 96 h. Relative cell number was analyzed by measuring the DNA content of cell lysates with Hoechst dye 33258-based 96-well proliferation assay (Sigma, St. Louis, MO, USA) as described previously [10 (link)]. All readouts were normalized to the average of the control condition in each individual experiment. The experiment was repeated at least three times. Eight wells were used for each condition.
Hoechst dye 33258 based 96 well proliferation assay
Manufactured by Merck Group
Sourced in United States
The Hoechst dye 33258-based 96-well proliferation assay is a laboratory tool used to measure cell proliferation. It utilizes the Hoechst 33258 dye, which binds to DNA, to quantify the amount of DNA present in a sample as an indicator of cell number.
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Lab products found in correlation
2 protocols using hoechst dye 33258 based 96 well proliferation assay
1
Cell Proliferation Assay with GRT
2
Docetaxel and CAPE Cytotoxicity Assay
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PC/DX25 and DU/DX50 cells were seeded at a density of 3 × 103 cells/well in 96-well plates with 100 μl RPMI-1640 medium containing 10% FBS and increasing concentration of docetaxel and CAPE for 96 h. Relative cell number was analyzed by measuring the DNA content of cell lysates with Hoechst dye 33,258-based 96-well proliferation assay (Sigma, St. Louis, MO, USA) as described previously [17 (link)]. All readouts were normalized to the average of the control condition in each individual experiment. The experiment was repeated at least eight times.
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