Proteins were loaded onto a 10% SDS-PAGE gel and separated using Tris-glycine SDS-PAGE running buffer. PageRuler Plus pre-stained protein ladder (26619, ThermoFisher Scientific) was used to indicate the molecular weight of target proteins. After electrophoresis, proteins were transferred to 0.45 μM nitrocellulose membranes (1620115, BioRad) with Towbin transfer buffer. Membranes were blocked with 5% bovine serum albumin and then incubated with corresponding primary antibodies. Membranes were then washed with Tris-buffered saline with 0.5% Tween 20 and incubated with IRDye secondary antibodies. Membranes were scanned and images were collected on an Odyssey CLX (LICOR) imaging system. Membranes were stripped with ReBlot Plus strong antibody stripping solution (2504, Millipore) for the detection of other targets, if necessary. Anti-IRS1 (2390), anti-IRS2 (3089), and anti-PTEN (9559) antibodies were purchased from Cell Signaling Technology. Anti-β-actin (A5441) and anti-Vinculin (V9131) antibodies were purchased from Sigma-Aldrich.
Anti pten 9559
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-PTEN (9559) is a primary antibody that specifically recognizes the PTEN protein. PTEN is a tumor suppressor protein that plays a critical role in regulating cell proliferation and survival. This antibody can be used to detect and analyze PTEN expression in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin a5441, by Merck Group (1 mentions)
Reblot plus strong antibody stripping solution, by Merck Group (1 mentions)
Anti vinculin v9131, by Merck Group (1 mentions)
Pageruler plus prestained protein ladder, by Thermo Fisher Scientific (1 mentions)
Odyssey clx, by LI COR (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
Anti bcl 2 2876, by Cell Signaling Technology (1 mentions)
Anti phospho akt 9271, by Cell Signaling Technology (1 mentions)
Anti beta actin clone ac 15, by Merck Group (1 mentions)
2 protocols using anti pten 9559
1
Western Blotting for Protein Analysis
2
Western Blot Analysis of Tbx3, PTEN, Bcl-2, and Phospho-Akt
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For western blotting analysis, anti-Tbx3 antibody was purchased from Novus Biologicals (H00006926-A01; Littleton, CO, USA); anti-PTEN (9559), anti-Bcl-2 (2876) and anti-phospho-Akt (9271) were all purchased from Cell Signaling Biotechnology (Danvers, MA, USA). Anti-flag tag antibody (F7425) and anti-beta-actin (clone AC-15) were from Sigma-Aldrich (St. Louis, MO, USA). Synthetic miR-206 mimic, antagomiR-206 (αmiR-206) and miR scrambled control (NC) were purchased from Qiagen (Valencia, CA, USA). pMIR-REPORT Luciferase vector and control vector were purchased from Applied Biological Materials (ABM MT-h25249; Richmond, BC, Canada), SMART pool: ON-TARGETplus, Tbx3-siRNA (LU-012197-00-0005) and set of four individual siRNA (LU-012197-000002) were purchased from Dharmacon (Lafayette, CO, USA). Human Tbx3 flag-tagged cDNA (RC220990) was from OriGene Technologies, Inc. (Rockville, MD, USA).
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