At weeks 0, 4, 8, and 11, blood glucose readings were measured after overnight fasting (16 h). Blood was collected from the lateral tail vein and immediately assessed for glucose levels using the Precision Xtra Blood Glucose and Ketone Monitoring System (Abbott Diabetes Care, Alameda, CA, USA).
At week 11, glucose tolerance testing (GTT) was performed. First, baseline fasting glucose levels were taken. Mice were then administered a glucose bolus (2 g/kg body weight) via IP injection. Blood glucose levels were recorded from lateral tail vein 15, 30, 60, and 120 min after the glucose bolus. Area under the curve (AUC) was calculated.
Plasma cholesterol and triglyceride levels were measured enzymatically at the conclusion of the experimental period. Commercially available kits for both cholesterol (Total Cholesterol Colorimetric Assay kit, #K603, BioVision, Milpitas, CA, USA) and triglycerides (LabAssay Triglycerides, #290-63701, Wako Chemicals, Richmond, VA, USA) were used following the manufacturers’ protocols.
At week 11, glucose tolerance testing (GTT) was performed. First, baseline fasting glucose levels were taken. Mice were then administered a glucose bolus (2 g/kg body weight) via IP injection. Blood glucose levels were recorded from lateral tail vein 15, 30, 60, and 120 min after the glucose bolus. Area under the curve (AUC) was calculated.
Plasma cholesterol and triglyceride levels were measured enzymatically at the conclusion of the experimental period. Commercially available kits for both cholesterol (Total Cholesterol Colorimetric Assay kit, #K603, BioVision, Milpitas, CA, USA) and triglycerides (LabAssay Triglycerides, #290-63701, Wako Chemicals, Richmond, VA, USA) were used following the manufacturers’ protocols.