Uas orco

Flies were raised on ethanol-free standard cornmeal/molasses/yeast/agar medium at 25°C and 60% relative humidity under a 12 h light/12 h dark cycle. The following lines were used: w¹¹¹⁸; Orco¹ (Larsson et al., 2004 (link)); w*; Orco-GAL4 [Bl #26818] and w*; UAS-Orco [Bl#23145] from the Bloomington Drosophila Stock Center. All strains were backcrossed to w¹¹¹⁸ (Scholz lab) for at least five generations.

Fly strains used in the present study were as follows: Orco-Gal4 (Bloomington #26818), UAS-GCaMP6f (Bloomington #42747), w⁻;; w⁺, orco¹ (null allele, Bloomington #23129), Or42a-Gal4 (Bloomington #9970), Or42b-Gal4 (Bloomington #9972), UAS-Orco (second, this study), UAS-CsChrimson (Bloomington #55135), Tsh-Gal80 (second, kindly gifted by Dr. Miesenböck), UAS-RGECO1 (our stock)⁴³ , UAS-hChR2[H134R] (our stock)⁴³ , Orco-ChR2 (Bloomington #63041)^{44 (link)}, GH146-Gal4 (Bloomington #30026).

All flies were raised on standard cornmeal agar medium, under 60% humidity and a 12-h light/12-h dark cycle at 25 °C. The Or10a-Gal4 (BL9944), Or42b-Gal4 (BL9971), Or43a-Gal4 (BL9974), Or49b-Gal4 (BL9986), Or56a-Gal4 (BL9988 and 23896), Or82a-Gal4 (BL23125), Or85a-Gal4 (BL23133), Or92a-Gal4 (BL23140), UAS-Orco (BL23145), Orco^{1 (link)} (BL23129), Orco^{2 (link)} (BL23130), UAS-TNT (BL28837 and 28997), UAS-TNT (inactive, BL28844), UAS-GCaMP6m (BL42748 and 42750), UAS-GCaMP6f (BL42747), and UAS-H134R-ChR2 (BL28995) flies were from the Bloomington Stock Center. UAS-mCD8-GFP was a gift from Dr. Chris Potter at the Johns Hopkins University School of Medicine, USA. UAS-P2X₂ was a gift from Dr. Zuoren Wang at the Institute of Neuroscience, China. The flies have been backcrossed for seven generations to a laboratory w¹¹¹⁸ strain.