Rmg 1 cells

Human ovarian clear cell carcinoma ES-2 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in MaCoy’s 5A medium (Life Technologies, Carlsbad, CA, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; SAFC Biosciences, Lenxa, KS, USA) and 1% (v/v) penicillin-streptomycin (PS) (Life Technologies). Human ovarian mesonephroid adenocarcinoma RMG-I cells were purchased from Japanese Collection of Research Bioresources (JCRB, Osaka, Japan) and maintained in Ham’s F12 medium (Life Technologies) supplemented with 10% (v/v) heat-inactivated FBS (Life Technologies) and 1% (v/v) PS. Human sarcoma HT-1080 cells were purchased from JCRB and maintained in minimal essential medium (Life Technologies) supplemented with 10% (v/v) heat-inactivated FBS, 1% (v/v) non-essential amino acids (Life Technologies), 2% (v/v) sodium bicarbonate (final concentration, 0.15%; Life Technologies) and 1% (v/v) PS. Human colon adenocarcinoma DLD-1 cells were purchased from JCRB and maintained in RPMI-1640 medium (Life Technologies) supplemented with 10% (v/v) heat-inactivated FBS, 1% (v/v) HEPES (Life Technologies) (final 20 mM), 1% (v/v) sodium pyruvate (Life Technologies), 0.62% (v/v) glucose (Sigma-Aldrich, St. Louis, MO, USA) and 1% (v/v) PS. Cells were incubated at 37 °C in an air-conditioned atmosphere with 5% CO₂.

Human ovarian cancer cell lines, SKOV3, OVCA3, OVCA433, and OVCA429 were obtained from the American Type Culture Collection (Manassas, VA, USA). SNU-840 cells were purchased from Korea Cell Line Bank (KCLB, Seoul, Korea). RMG-I cells were purchased from Japanese Collection of Research Bioresources Cell Bank (JCRB, Tokyo, Japan). All purchased cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS; HyClone, UT, USA) and 1% antibiotics (Cellgro, Manassas, VA, USA). Six ovarian cancer cell lines (YDOV-139, YDOV-157, YDOV-161, YDOV-13, YDOV-105, and YDOV-151) and four HOSE cell lines (HOSE 198, 209, 211, and 213), which had been previously established [24 (link)], were maintained in a 1:1 mixture of Medium 199 and MCDB 105 (Sigma, St. Louis, MO, USA) supplemented with 10% FBS (Gemini Bio-Products, Calabasas, CA, USA). Immortalized HOSE8695 (IHOSE8695) cells had been established previously [38 (link)] and were maintained in DMEM supplemented with 10% FBS (HyClone, UT, USA) and 1% antibiotics (Cellgro, Manassas, VA, USA). All cell lines were incubated at 37 °C in a humidified incubator with 5% CO₂. All-trans retinoic acid (ATRA) and 5-aza-2′-deoxycytidine (5-Aza-CdR) were purchased from Sigma (St. Louis, MO, USA).