Apc mouse antihuman cd73

Manufactured by BD

Sourced in United States

The APC mouse antihuman CD73 is a laboratory reagent used for the detection and analysis of CD73-positive cells in flow cytometry applications. CD73 is a cell surface enzyme that plays a role in the regulation of extracellular adenosine levels. This reagent can be used to identify and quantify CD73-expressing cells in various research contexts.

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CD73-APC (21 citations)

2 protocols using apc mouse antihuman cd73

Phenotyping Mesenchymal Stem Cells by FACS

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The cell phenotype was assessed by fluorescence-activated cell sorting (FACS) on BD FACSAria flow cytometer (BD Pharmingen, BD Horizon USA). Staining with monoclonal antibodies (PerCP-Cy5.5 mouse antihuman CD105, APC mouse antihuman CD73, FITC mouse antihuman CD90, PE-Cy5 mouse antihuman HLA-DR, APC mouse antihuman CD34, and FITC mouse antihuman CD45) in accordance with manufacturer's instructions (BD Pharmingen, BD Horizon USA). The analysis was performed using BD FACS Diva 6.1 software (BD Pharmingen, BD Horizon USA).

Zlatska A., Gordiienko I., Vasyliev R., Zubov D., Gubar O., Rodnichenko A., Syroeshkin A, & Zlatskiy I. (2018). In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells. The Scientific World Journal, 2018, 5454367.

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Surface Marker Analysis of Mesenchymal and Cancer Cells

Cited 1 time

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Surface marker analysis by FACS was carried out as described previously [16 (link)]. In brief, hMSC and ES cell lines (RD-ES, SK-N-MC and EWS-GFP) were harvested, centrifugated and incubated at 4°C for 1 h with fluorochrome conjugated antibodies APC Mouse anti-human CD13 (BD Pharmingen, 557454), APC Mouse anti-human CD44 (BD Pharmingen, 560532), APC Mouse anti-human CD73 (BD Pharmingen, 560847), APC Mouse anti-human CD90 (BD Pharmingen, 559869) and APC Mouse anti-human CD105 (BD Pharmingen, 562408). Negative control cells were stained with APC mouse IgG1, k isotype control, Clone MOPC-21 (BD Pharmingen, 555751). CD99 expression was assayed incubating cells with CD99 primary antibody (Signet antibodies, SIG-3620). FACS data were analyzed using FlowJo software version 7.6 (Tree Star Inc., Ashland, OR, USA)

Villasante A., Marturano A, & Vunjak-Novakovic G. (2014). Bioengineered human tumor within a bone niche. Biomaterials, 35(22), 5785-5794.

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