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Anti cd4 bd605nc

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The Anti-CD4-BD605NC is a fluorochrome-conjugated antibody that specifically binds to the CD4 surface antigen. It is designed for use in flow cytometry applications to identify and characterize CD4-positive cells.

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Lab products found in correlation

Lsr 2 flow cytometer, by BD (2 mentions) Celltrace violet, by Thermo Fisher Scientific (2 mentions) Anti cd8 apc efluor780, by Thermo Fisher Scientific (2 mentions) Anti cd62l pe cy7, by Thermo Fisher Scientific (2 mentions) Anti cd44 apc, by Thermo Fisher Scientific (2 mentions) Apc brdu flow kit, by BD (2 mentions) Moflow, by Beckman Coulter (2 mentions) Anti cd69 percpcy5, by Thermo Fisher Scientific (2 mentions) Anti cd98 pe, by Thermo Fisher Scientific (2 mentions)

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Anti-CD4 (255 citations)

2 protocols using anti cd4 bd605nc

1

Multiparameter Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies from eBiosciences included anti-CD8-APC-eFluor780 (47-0081), anti-CD44-APC (17-0441-81), anti-CD69-PERCPcy5.5 (45-0691), anti CD98-PE (12-0981-81; eBiosciences), and anti CD62L-PEcy7 (25-0621). Anti-CD4-BD605NC was acquired from BD Biosciences (San Jose, CA). Cells were stained for 20 minutes at 4°C in PBS, 5% BSA, 0.1% NaN3. Cell proliferation was assessed using Cell Trace Violet (C34557; Life Technologies) according to the manufacturer’s recommendations. Samples were acquired on a BD LSRII flow cytometer and analyzed with Treestar FlowJo software. Cell cycle analysis was performed using BD APC BrdU Flow kit per the manufacturer’s instructions (552598; BD Biosciences). Cells were sorted on a MoFlow (Beckman-Coulter) or Reflection (i-Cyt).
Verbist K.C., Guy C.S., Milasta S., Liedmann S., Kamiński M.M., Wang R, & Green D.R. (2016). Metabolic Maintenance of Cell Asymmetry following Division in Activated T Lymphocytes. Nature, 532(7599), 389-393.
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2

Multiparameter Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies from eBiosciences included anti-CD8-APC-eFluor780 (47-0081), anti-CD44-APC (17-0441-81), anti-CD69-PERCPcy5.5 (45-0691), anti CD98-PE (12-0981-81; eBiosciences), and anti CD62L-PEcy7 (25-0621). Anti-CD4-BD605NC was acquired from BD Biosciences (San Jose, CA). Cells were stained for 20 minutes at 4°C in PBS, 5% BSA, 0.1% NaN3. Cell proliferation was assessed using Cell Trace Violet (C34557; Life Technologies) according to the manufacturer’s recommendations. Samples were acquired on a BD LSRII flow cytometer and analyzed with Treestar FlowJo software. Cell cycle analysis was performed using BD APC BrdU Flow kit per the manufacturer’s instructions (552598; BD Biosciences). Cells were sorted on a MoFlow (Beckman-Coulter) or Reflection (i-Cyt).
Verbist K.C., Guy C.S., Milasta S., Liedmann S., Kamiński M.M., Wang R, & Green D.R. (2016). Metabolic Maintenance of Cell Asymmetry following Division in Activated T Lymphocytes. Nature, 532(7599), 389-393.
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