miR-190a-3p mimic/miR-NC mimic and miR-190a-3p inhibitor/miR-NC inhibitor were purchased from Shanghai GenePharma (Shanghai, China). Their sequences were listed as follow: miR-190a-3p mimic: 5ʹ-CUAUAUAUCAAACAUAUUCCU-3ʹ; miR-NC mimic: 5ʹ-UCGCUUGGUGCAGGUCGGGAA-3ʹ; miR-190a-3p inhibitor: 5ʹ-AGGAAUAUGUUUGAUAUAUAG-3ʹ; miR-NC inhibitor: 5ʹ- UUCUCCGAACGUGUCACGUTT-3ʹ. miR-190a-3p mimic/miR-NC mimic and miR-190a-3p inhibitor/miR-NC inhibitor were transfected into U87MG cells and LN-18 cells at the work concentration of 80 nM with Lipofectamine 3000 reagent (Invitrogen) following the manufacturer’s protocol. After 48 h, the cells were harvested and subjected to RT-qPCR or Western blotting.
Mir 190a 3p mimic
Manufactured by Thermo Fisher Scientific
The MiR-190a-3p mimic is a synthetic double-stranded RNA molecule designed to mimic the endogenous microRNA (miRNA) MiR-190a-3p. MiR-190a-3p is a small non-coding RNA that plays a role in gene expression regulation.
Automatically generated - may contain errors
1 protocol using mir 190a 3p mimic
1
Regulating miR-190a-3p in Glioma Cells
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