Ethylene glycol

Sodium alginate, sodium periodate and ethylene glycol were purchased from J&K Scientific Ltd. (Beijing, China). Polyethyleneimine solution was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Opti-MEM reduced-serum medium was supplied by Life Technologies (Carlsbad, CA, USA). Lipofectamine 2000 was provided by Invitrogen (New York, NY, USA). Sulforhodamine B was purchased from Macklin Ltd. (Shanghai, China). dsRNA (sense strand: 5′-UGGCAGUGUCUUAGCUGGUUGU-3′ and antisense strand: 5′-AACCAGCUAAGACACUGCCAUU-3′), ssRNA (inhibitor) (strand: 5′-ACAACCAGCUAAGACACUGCCA-3′), negative control (sense strand: 5′-UUCUCCGAACGUGUCACGUTT-3′ and antisense strand: 5′-ACGUGACACGUUCGGAGAATT-3′, named as NC), fluorescein-labeled dsRNA (5′ end of the sense strand, FAM-dsRNA), fluorescein-labeled ssRNA inhibitor (5′ end of the sense strand, FAM-ssRNA), and fluorescein-labeled negative control (5′ end of the sense strand, FAM-NC) were synthesized and purified with HPLC by Sangon Biotech Co., Ltd. (Shanghai, China). DMEM medium and PRMI 1640 medium were obtained from Macgene Technology Co., Ltd. (Beijing, China). The solvents were purchased from J&K Scientific Ltd. (Beijing, China).