Avance 3 300 mhz spectrometer

Manufactured by Bruker

Sourced in United States

The AVANCE III 300 MHz spectrometer is a nuclear magnetic resonance (NMR) instrument designed for routine analysis and basic research applications. It provides a magnetic field strength of 300 MHz for the proton (1H) nucleus. The spectrometer is equipped with a high-performance console and supports a range of NMR experiments and data processing capabilities.

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Lab products found in correlation

Uv 2550 spectrophotometer, by Shimadzu (1 mentions) Avance 3 700 mhz spectrometer, by Bruker (1 mentions) Avance 3 500 mhz spectrometer, by Bruker (1 mentions) Propylamine hydrochloride, by Merck Group (1 mentions) Formaldehyde, by Avantor (1 mentions) Butylamine, by Merck Group (1 mentions) Alpha ftir, by Bruker (1 mentions) 2 ethylhexylamine, by Merck Group (1 mentions) La no3 3 6h2o, by Merck Group (1 mentions) Y no3 3 6h2o, by Merck Group (1 mentions) Topspin 4, by Bruker (1 mentions) Optima8300 dv icp oes spectrometer, by PerkinElmer (1 mentions) Calcium calibration buffer kit, by Thermo Fisher Scientific (1 mentions) Fp 8300 spectrofluorometer, by Jasco (1 mentions) Inova 500 mhz nmr spectrometer, by Agilent Technologies (1 mentions) Topspin 3, by Bruker (1 mentions) Perkin elmer analyzer, by PerkinElmer (1 mentions) 60 f254 plate, by Merck Group (1 mentions) Microtof qii, by Bruker (1 mentions) Nicolet is5 spectrometer, by Thermo Fisher Scientific (1 mentions)

16 protocols using avance 3 300 mhz spectrometer

MRI Analysis of Porous Structures

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MRI experiments were performed by the Bruker Avance
III 300 MHz spectrometer equipped with Micro2.5 microimaging unit
with x, y, and z gradients, using a 10 mm RF insert. The multi-slice multi-echo (MSME)
sequence was used in the experiments. The number of echoes was 16,
and the echo time was 20 ms. Altogether eight slices were measured
from each sample with 500 μm slice thickness and a field of
view of 1 cm. The image was acquired on the grid of 300 × 300
points, and the size of individual pixel was 33 μm. The image
was averaged 16 times with the repetition time of 48 s. The axial
images were acquired for all four samples, and for the 1% consistency
sample, an additional coronal image was acquired.
After the
acquisition, the analysis was performed in MATLAB software. For each
pixel the T₂ value was calculated. Then
all the pixels with T₂ within the range
350–650 ms where assigned as macro-pores and selected for further
analysis. The selected pixels where grouped according to connections
with other pixels to separate shapes corresponding to single pore.
For each pore the ellipsis was fitted to determine the length, width
and angle of main axis. The analysis was possible by using MATLAB
functions “bwlabel” and “regionprops”
from Image processing toolbox. The pore diameter was defined to be
a mean of the longer and shorter axis of the ellipsis.

Kharbanda Y., Urbańczyk M., Laitinen O., Kling K., Pallaspuro S., Komulainen S., Liimatainen H, & Telkki V.V. (2019). Comprehensive NMR Analysis of Pore Structures in Superabsorbing Cellulose Nanofiber Aerogels. The Journal of Physical Chemistry. C, Nanomaterials and Interfaces, 123(51), 30986-30995.

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Comprehensive Analytical Characterization

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The fluorescence spectra were recorded with a Thermo Scientific Lumina fluorescence spectrophotometer (Thermo Fisher Scientific Co., USA). The UV-Vis absorption spectra were measured on a UV-2550 spectrophotometer (Shimadzu Co., Japan). The 1 H NMR spectra were carried out on a Bruker Avance III 300 MHz spectrometer (Bruker Co., Switzerland).
The MS spectra were obtained on TSQ quantum-HPLC/MS spectrometer (Finnigan Co., USA).

Lan T., Wang F.H., Xi X.J., Cheng C.W., Lei W., Xia M.Z, & Wang F.Y. (2016). A Rhodamine-based Dual Chemosensor for the Simultaneous Detection of Fe³⁺ and Cu². Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 32(11).

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NMR Spectroscopy of Organic Compounds

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All chemicals were purchased from Sigma-Aldrich at >98% purity. Approximately 20 mg of the sample were dissolved in 500 µL of deuterated solvent ( pyridine, DMSO, acetone, acetonitrile, chloroform or methanol). For protonation/deuteration of heteroatoms, 50 µL of water or deuterated water were added. TMS was added for chemical shift referencing. In the case of methanol, methanol-d 3 was used for samples with water and methanol-d 4 was used for the samples with deuterated water. For the phosphate esters, ACN-d 3 was used as solvent and triphenylphosphate was added for chemical shift referencing. All samples were measured in duplicates. In case of the flavonol glycoside rutin, 5 mg were dissolved in 600 µL pyridine-d 5 and 10% or 2% of water or deuterated water were added. The experiments were recorded on a Bruker Avance III 700 MHz spectrometer at 298 K.
All other NMR spectra were recorded on a Bruker AVANCE III 300 MHz spectrometer or on a Bruker Avance III 500 MHz spectrometer using a 5 mm TXI probe with z-axis gradients at 300 K. Typically, 512 scans and 32k data points were acquired for proton decoupled carbon-spectra. Spectra were processed using MestreNova software.

Tassoti S ., Walenta M ., Pöcheim A ., Buchberger K ., Kunert O , & Zangger K . (2019). Solvent-independent determination of heteroatom protonation states from NMR spectra by differential deuterium isotope shifts. The Analyst, 144(24).

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Lanthanoid Complexes Synthesis and Characterization

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Formaldehyde (36%) was purchased
from VWR; phosphorous acid (99%) and hexylamine (98%) from Fluka Chemical
Co.; 2-ethylhexylamine (98%), La(NO₃)₃·6H₂O, and propylamine hydrochloride from Sigma-Aldrich; ethylamine
hydrochloride (98%), butylamine (99%), and Y(NO₃)₃·6H₂O (99.8%) from Merck; and Lu(NO₃)₃·H₂O from abcr and amylamine (98%) from TCI
chemicals. All of the chemicals were reagent grade and used without
further purification. NMR measurements and titrations were performed
on a Bruker Avance III 300 MHz-spectrometer, and NMR data was processed
with Bruker TopSpin 4.0.8. IR spectra were measured by Bruker Alpha
FT-IR. Elemental analyses were done by an Elementar Vario EL III-analysator.
Lanthanoid concentrations were determined by a Perkin Elmer Optima
8300 DV ICP-OES- spectrometer.

Virtanen E.J., Perämäki S., Helttunen K., Väisänen A, & Moilanen J.O. (2021). Alkyl-Substituted Aminobis(phosphonates)—Efficient Precipitating Agents for Rare Earth Elements, Thorium, and Uranium in Aqueous Solutions. ACS Omega, 6(37), 23977-23987.

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Spectroscopic Characterization of Fluorescent Dyes

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All the solvents were of analytical grade. Chemicals were purchased from commercial sources. ¹H-NMR and ¹³C-NMR were measured on a Bruker Avance III-300 MHz spectrometer (Bruker Biospin, The Woodlands, TX, USA) with chemical shifts reported in ppm (TMS as internal standard). Mass spectra were measured on a Focus GC/DSQ II spectrometer (ThermoScientific, Waltham, MA, USA) for IC and an API 3000 spectrometer (Applied Biosystems, PE Sciex) for ES. All pH measurements were made with a Mettler Toledo pH meter. Fluorescence spectra were recorded on a JASCO FP-8300 spectrofluorometer (JASCO, Easton, MD, USA). Absorption spectra were determined on a VARIAN CARY 300 Bio UV-Visible spectrophotometer. All measurements were done at a temperature of 25°C. The purity of the dyes were checked by RP-HPLC C-18, elutant: ACN 0.1% TFA/Water 0.1% TFA, method: 20/80 to 100/0 within 20 min then 100/0 for 10 min detection at λ_Abs = 254 nm. The apparent dissociation constant for calcium (K_D Ca²⁺) was measured with a calcium calibration buffer kit from Invitrogen (Lifetechnologies, USA). All mass spectra, NMR spectra and chromatograms are included as supplemental data.

Collot M., Wilms C.D., Bentkhayet A., Marcaggi P., Couchman K., Charpak S., Dieudonné S., Häusser M., Feltz A, & Mallet J.M. (2015). CaRuby-Nano: a novel high affinity calcium probe for dual color imaging. eLife, 4, e05808.

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Lyophilized Sample NMR Preparation

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Lyophilized products were dissolved to 5.00−100 mM in D₂O in a total volume of 600 μL and transferred to a 5 mm high-precision NMR sample tube. Samples were analyzed on a Varian INOVA 500-MHz NMR spectrometer (Agilent Technologies, Santa Clara, California, USA) using the VNMRJ 2.2D software for the measurements, (Agilent Technologies, Santa Clara, CA, USA) or on a Bruker AVANCE III 300-MHz spectrometer (Bruker, Rheinstetten, Germany) with an autosampler and the Bruker Topspin 3.5 software for measurements.

Pfeiffer M., Ribar A, & Nidetzky B. (2023). A selective and atom-economic rearrangement of uridine by cascade biocatalysis for production of pseudouridine. Nature Communications, 14, 2261.

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Characterization of Organic Compounds Using Analytical Techniques

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All commercial reagents and solvents were purchased from Sigma-Aldrich Corp. (St.Louis, MO, USA) and used without purification. Reactions were routinely monitored by thin-layer chromatography (TLC) in silica gel (60 F₂₅₄ plates Merck, Darmstadt, Germany) and the products were visualized with ultraviolet light of 254 nm wavelength. All NMR spectra were acquired on an AVANCE III 300 MHz spectrometer (Bruker Bioscience, Billerica, MA, USA) equipped with a BBO Z-gradient probe. Spectra were recorded at 25 °C using DMSO as a solvent with a non-spinning sample in 5 mm NMR-tubes. MS spectra were recorded on a Bruker microTOF-Q II (Bruker Bioscience, Billerica, MA, USA) and processed using Compass Data Analysis software (Brucker ASX, Karlsruhe, Germany). The elementary analysis was performed with the application of a Perkin-Elmer analyzer (Perkin Elmer Inc., Waltham, MA USA). Melting points were determined with a Boetius apparatus (Jena, Germany).

Szacoń E., Rządkowska M., Kaczor A.A., Kędzierska E., Orzelska-Górka J., Fidecka S, & Matosiuk D. (2016). Synthesis and Pharmacological Evaluation of Novel 1-(1,4-Alkylaryldisubstituted-4,5-dihydro-1H-imidazo)-3-substituted Urea Derivatives. Molecules, 21(5), 582.

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Comprehensive Analytical Characterization

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All of the
NMR spectra were collected on a Bruker AVANCE III 300
MHz spectrometer. Structural assignments were made with additional
information from gCOSY, gHSQC, and gHMBC experiments. All attenuated
total reflectance IR (ATR-IR) spectra were measured on the Thermo
Scientific NICOLET iS5 spectrometer using the iD5 ATR interface. Melting
points were measured on a polarized light microscope (Axioscope A1
Pol) using a thermostatic interface (LINKAM LTSE420). The high-resolution
mass spectrometry (HRMS) data were determined on a Bruker Daltonics
micrOTOF-Q II spectrometer.

Ręka P., Grolik J., Stadnicka K.M., Kołton-Wróż M, & Wołkow P. (2023). Synthesis of Nonsymmetrically Substituted 2,3-Dialkoxyphenazine Derivatives and Preliminary Examination of Their Cytotoxicity. The Journal of Organic Chemistry, 88(3), 1339-1351.

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Synthesis of Molybdenum Carbonyl Complexes

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Synthetic manipulations were performed under a nitrogen atmosphere using standard Schlenk and glove-box techniques. Solvents were purified via a Pure Solv Solvent Purification System. Chemicals were purchased from commercial sources and used without further purification. The precursor MoI₂(CO)₃(NCMe)₂ was synthesized according to a literature procedure (Baker et al., 1986 ▸ ). For the synthesis of Mo(CO)₂(S-Phoz)₂, a slight modification of a published procedure was used (Peschel et al., 2013 ▸ ). ¹H NMR spectra were recorded on a Bruker Avance III 300 MHz spectrometer at ambient temperature and are referenced to residual protons in the solvent. The multiplicity of peaks is denoted as singlet (s), doublet (d), doublet of doublets (dd) or multiplet (m). NMR solvents were stored over molecular sieves. Solid-state IR spectra were measured on a Bruker ALPHA ATR–FT–IR spectrometer at a resolution of 2 cm⁻¹. The relative intensity of signals is declared as strong (s), medium (m) and weak (w). Electron impact mass spectroscopy (EI–MS) measurements were performed with an Agilent 5973 MSD mass spectrometer with a push rod.

Ehweiner M.A., Belaj F, & Mösch-Zanetti N.C. (2022). Synthesis and structure of two isomers of a molybdenum(II) 2-butyne complex stabilized by bioinspired S,N-bidentate ligands. Acta Crystallographica. Section C, Structural Chemistry, 78(Pt 4), 218-222.

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Characterization of Organic Compounds

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All chemicals and reagents used in current study were obtained from commercial sources and used without further purification. All melting points were determined on Electrothermal-9100 apparatus and are uncorrected. IR spectra were recorded on a Bruker FTIR (Alpha model) spectrophotometer using KBr pallets. 1 (link) H NMR (300 MHz) and 13 (link) C NMR (75 MHz) spectra were recorded on a Bruker AVANCE III 300 MHz spectrometer in DMSO-d 6 , with TMS as an internal standard. Chemical shifts (δ) are given in parts per million (ppm) and coupling constants (J) are given in Hertz (Hz). Reactions were monitored by thin layer chromatography (TLC) on the Aluminium-backed silica gel sheets (GF254) and visualized in UV light (254 nm). Elemental analyses were performed using a Heraeus CHN-O-Rapid analyzer.

Sabouri S., Faghih-Mirzaei E, & Abaszadeh M. (2022). Synthesis and In Vitro Cytotoxicity of Novel Halogenated Dihydropyrano[3,2-b]Chromene Derivatives. Acta chimica Slovenica, 69(4).

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