The structural changes in cellulose after enzymatic treatment was confirmed by the FTIR spectroscopy. The spectra were obtained using a pelleting technique—1 mg cellulose was mixed with 200 mg bromide potassium (KBr, Sigma-Aldrich, Steinchein, Germany). Spectra were registered using an FTIR Nicolet iS5 spectrometer (Thermo Fisher Scientific, Madison, WI, USA) at a range from 4000 to 500 cm−1 at a resolution of 4 cm−1, registering 16 scans.
Ft ir nicolet is5 spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FT-IR Nicolet iS5 spectrometer is a compact and robust Fourier Transform Infrared (FT-IR) spectrometer designed for a wide range of analytical applications. It utilizes infrared light to identify and quantify the chemical composition of samples in solid, liquid, or gas form.
Automatically generated - may contain errors
Lab products found in correlation
Silica gel 60n, by Kanto Chemical (2 mentions)
Jms 700 spectrometer, by JEOL (2 mentions)
Ods silica gel gel ods a, by YMC (2 mentions)
Thioflavin t, by Fujifilm (2 mentions)
Synergy htx multi mode reader, by Agilent Technologies (2 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
Genesys 10s uv vis spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Pure c 850 flashprep, by Büchi (1 mentions)
Ammonia solution, by Fujifilm (1 mentions)
Jnm al 400 mhz, by JEOL (1 mentions)
Dowex 50wx8 resin, by Merck Group (1 mentions)
Sepa 300 polarimeter, by Horiba (1 mentions)
Tlc silica gel 60 f254, by Merck Group (1 mentions)
Diaion hp 20, by Mitsubishi (1 mentions)
Rp 18 f254, by Merck Group (1 mentions)
4 protocols using ft ir nicolet is5 spectrometer
1
Cellulose Structural Changes via FTIR
2
Spectroscopic Analysis and Amyloid Aggregation Inhibition
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IR spectra were measured using a Thermo Scientific FT-IR Nicolet iS5 spectrometer (ATR). UV spectra were measured using a Thermo Scientific UV–Vis GENESYS 10S spectrophotometer. ECD spectra were measured with a Jasco J-820 circular dichroism spectropolarimeter (JASCO, Tokyo, Japan). 1H- and 13C-NMR spectra were recorded using a JNM-AL 400 MHz and JNM-ECZ 400 MHz spectrometer (JEOL, Tokyo, Japan). HREIMS and HRFABMS spectra were obtained using a JEOL JMS-700 spectrometer (JEOL). Column chromatography was carried out with silica gel 60 N (63–210 μm, Kanto Chemical, Tokyo, Japan) and ODS silica gel YMC-GEL ODS-A (YMC, Kyoto, Japan). MPLC was performed using a Pure C-850 FlashPrep (BUCHI, Flawil, Switzerland) and a series of Flash Cartridges (BUCHI, Flawil, Switzerland). Semi-preparative HPLC was conducted using a Jasco PU-2080 Plus pump (JASCO) and a Jasco UV-2075 Plus UV/VIS detector at 220 or 254 nm (JASCO). Inhibition of Aβ aggregation was assayed using a Synergy HTX Multi-Mode Reader (BioTek, Winooski, VT, USA), Aβ40 peptide (Peptide Institute, Osaka, Japan), thioflavin-T (FUJIFILM Wako, Osaka, Japan), 25% ammonia solution (FUJIFILM Wako), and dimethyl sulfoxide (FUJIFILM Wako).
3
Temperature-Dependent FT-IR Spectroscopy
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The FT-IR Nicolet iS5 spectrometer (Thermo Scientific, Madison, WI, USA) was used to collect FT-IR spectra with a wavenumber ranging from 4000 cm−1 to 400 cm−1. To record the spectra at different temperatures, the samples were analyzed using the same spectrometer equipped with an attenuated total reflection accessory (Pike Technologies, Madison, WI, USA) on a heatable germanium crystal (up to 120 °C) in transmission mode (64 scans and 4 cm−1 resolution). The chosen temperature range was 25–100 °C, with a heating and cooling rate of 10 °C/min.
4
Characterization and Evaluation of Aβ Aggregation Inhibitors
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Optical rotation values were recorded with a Horiba SEPA-300 polarimeter. IR spectra were measured with a Thermo FT-IR Nicolet iS5 spectrometer (ATR). 1H- and 13C-NMR spectra were recorded using a JNM LA-500 spectrometer. HRFABMS spectra were obtained using a JEOL JMS-700 spectrometer. Column chromatography was carried out with silica gel 60 N (63–210 μm) from Kanto Chemical, ODS silica gel YMC-GEL ODS-A from YMC Co. Ltd., and Diaion HP-20 from Mitsubishi Chemical Co. Ion-exchange chromatography was performed using Dowex 50 W-X8 resin (50–100 mesh, H-form) from Sigma-Aldrich. Thin-layer chromatography was carried out using TLC silica gel 60F254 and RP-18 F254S plates from Merck. Inhibition of Aβ aggregation was assayed using a Synergy HTX Multi-Mode Reader (BioTek, Winooski, Vermont, USA), Aβ40 (Peptide Institute, Osaka, Japan), and thioflavin-T (Fujifilm-Wako).
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