Cu grid

Manufactured by Ted Pella

The Cu grid is a laboratory equipment used for specimen preparation and support in electron microscopy. It serves as a platform to hold thin samples for examination under an electron beam. The grid is typically made of copper and features a mesh-like structure to allow for the transmission of electrons through the specimen.

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Lab products found in correlation

Tecnai t12, by Thermo Fisher Scientific (1 mentions) Ultrascan 2k 2k ccd camera, by Ametek (1 mentions)

2 protocols using cu grid

HRTEM Analysis of Carbon Dot/CNT Complexes

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High-resolution transmission
electron microscopy (HRTEM) is carried out on an aberration-corrected
FEI Titan3 80–300 microscope at 300 keV electron energy. Samples
for HRTEM were prepared at room temperature in air by drop casting
of a diluted suspension of carbon dot/CNT complexes in water onto
an ultrathin amorphous carbon film (3 nm) on a holey carbon support
film mounted on a 400 μm mesh Cu grid (Ted Pella Inc.). HRTEM
images were evaluated by calculating their two-dimensional Fourier
transform (FT), which yields information on the crystal structure
(lattice parameters and crystal symmetry) of single carbon dot/CNT
complexes. The analysis was performed by comparing experimental FTs
and calculated diffraction patterns with Miller indices, where the
latter were obtained by using Jems (Java version of the electron microscopy
simulation) software.³⁰ On FT images, the
zero-order beam is indicated by a white circle.

Sciortino A., Ferrante F., Gonçalves G., Tobias G., Popescu R., Gerthsen D., Mauro N., Giammona G., Buscarino G., Gelardi F.M., Agnello S., Cannas M., Duca D, & Messina F. (2021). Ultrafast Interface Charge Separation in Carbon Nanodot–Nanotube Hybrids. ACS Applied Materials & Interfaces, 13(41), 49232-49241.

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VLP-Based Vaccine Immunogen Conjugation

Cited 2 times

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SpyCatcher-AP205 VLPs were expressed in bacteria and purified as described (48 (link), 88 (link)). SpyTagged SOSIP immunogens were incubated at a 2 to 3–fold molar excess with SpyCatcher-VLPs for 12 to 24 hours at room temperature in phosphate-buffered saline (PBS). Conjugated VLPs were separated from free Env trimers by SEC on a Superdex 200 (fig. S7D) or Superose 6 (fig. S7E) column. Conjugation of Env trimers was verified by SDS-PAGE (fig. S7C). Immunogen concentrations for immunizations were estimated by comparing to known amounts of the analogous unconjugated Env trimer on a SDS-PAGE gel.
Conjugated VLPs were examined by nsEM (fig. S7A and D). Purified VLPs were diluted to about 10 μg/mL immediately before adding 3 μL to a glow-discharged ultrathin C film on a holey carbon support film, 400 mesh, Cu grid (Ted Pella). After blotting, the grids were stained by uranyl acetate and then imaged using a FEI Tecnai T12 transmission electron microscope operating at 120 keV with a Gatan Ultrascan 2k × 2k CCD camera. Each image was collected using a 1 second exposure at about 2 μm defocus and 42,000× magnification, resulting in 2.5 Å per pixel.

Escolano A., Gristick H.B., Gautam R., DeLaitsch A.T., Abernathy M.E., Yang Z., Wang H., Hoffmann M.A., Nishimura Y., Wang Z., Koranda N., Kakutani L.M., Gao H., Gnanapragasam P.N., Raina H., Gazumyan A., Cipolla M., Oliveira T.Y., Ramos V., Irvine D.J., Silva M., West AP J.r., Keeffe J.R., Barnes C.O., Seaman M.S., Nussenzweig M.C., Martin M.A, & Bjorkman P.J. (2021). Sequential immunization of macaques elicits heterologous, neutralizing antibodies targeting the V3-glycan patch of HIV-1 Env. Science translational medicine, 13(621), eabk1533.

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