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Ebiogoh3

Manufactured by Thermo Fisher Scientific
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The EBioGoH3 is a laboratory equipment product from Thermo Fisher Scientific. It is designed to perform essential bioanalytical functions. The core function of the EBioGoH3 is to enable researchers to conduct various bioanalytical activities in a controlled environment.

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Lab products found in correlation

Cd31 fitc, by Thermo Fisher Scientific (1 mentions) Cd45 fitc, by Thermo Fisher Scientific (1 mentions) Sca 1 pe cy7, by BioLegend (1 mentions) Moflo xdp cell sorter, by Beckman Coulter (1 mentions) Ter119 fitc, by Thermo Fisher Scientific (1 mentions) Clone 30 f11, by Thermo Fisher Scientific (1 mentions) Cd49f pe, by Thermo Fisher Scientific (1 mentions) Facsaria, by BD (1 mentions)

Spelling variants of this product

CD49f-PE (clone eBioGoH3; (2 citations)

2 protocols using ebiogoh3

1

Isolation of Murine Prostate Cells

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Isolation of prostate cells from adult mice was performed using the published method. Single cells were resuspended in phosphate-buffered saline (PBS) with 3% fetal bovine serum (FBS) and incubated with antibodies on ice for 45 minutes. Antibodies are: CD49f-PE (1:160; clone eBioGoH3; eBioscience; cat no 12-0495-83); Sca-1-PE-Cy7 (1:500; clone E13-161.7; BioLegend; cat no 122514); Ter119-FITC (1:250; clone TER-119; eBioscience; cat no 11-5921-85); CD31-FITC (1:250; clone 390; eBioscience; cat no 11-0311-85); CD45-FITC (1:250; clone 30-F11; eBioscience; cat no 11-0451-85). Cell isolations were performed on a Moflo XDP cell sorter (Beckman Coulter).
Fan X., Bjerke G.A., Riemondy K., Wang L, & Yi R. (2019). A basal-enriched microRNA is required for prostate tumorigenesis in a Pten knockout mouse model. Molecular carcinogenesis, 58(12), 2241-2253.
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2

Isolation of Epidermal Stem Cells

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Dorsal epidermis was separated from the dermis by digestion with 0.25% trypsin overnight at 4°C to obtain a single-cell suspension. Cells were stained with anti-CD45 (30-F11), anti-CD34 (RAM34), and anti-ITGA6 (eBioGoH3; eBioscience or BD PharMingen). ITGA6+, CD45−, and CD34− cells were sorted with a FACS Aria (BD Biosciences) and used for further analyses.
Shibata S., Kashiwagi M., Morgan B.A, & Georgopoulos K. (2019). Functional interactions between Mi-2β and AP1 complexes control response and recovery from skin barrier disruption. The Journal of Experimental Medicine, 217(3), jem.20182402.
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