Brequinar

Manufactured by Selleck Chemicals

Brequinar is a lab equipment product designed for use in scientific and research settings. It is a chemical compound that functions as a dihydroorotate dehydrogenase (DHODH) inhibitor, which is a key enzyme involved in the de novo pyrimidine biosynthesis pathway. Brequinar can be utilized in various experimental and analytical applications where DHODH inhibition is of interest.

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Lab products found in correlation

Pitstop 2, by Merck Group (1 mentions) Leptomycin b, by Cayman Chemical (1 mentions) Erastin, by Bio-Techne (1 mentions) Lovastatin, by Selleck Chemicals (1 mentions) Verdinexor, by Cayman Chemical (1 mentions) Tipifarnib, by MedChemExpress (1 mentions) Methotrexate, by Selleck Chemicals (1 mentions) Alisertib, by Selleck Chemicals (1 mentions) Fin56, by Cayman Chemical (1 mentions) Dorsomorphin, by Selleck Chemicals (1 mentions) Celltrace cfse cell proliferation kit, by Thermo Fisher Scientific (1 mentions) Lometrexol, by Cayman Chemical (1 mentions) Tetrahydrofolate, by Merck Group (1 mentions) Uridine, by Merck Group (1 mentions) Dipyridamole, by Merck Group (1 mentions)

3 protocols using brequinar

Ferroptosis Inducers and Inhibitors Screening

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erastin (Bio-techne, 5449); SAS (MedchemExpress, HY-14655); RSL3 (Cayman, 19288); FIN56 (Cayman, 25180); FINO2 (MedchemExpress, HY-129457); PANKi (Cayman, 31002); BSO (Sigma, B2515); DEM (Sigma, D97703); etomoxir sodium salt (Selleckchem, S8244); lovastatin (Selleckchem, S2061); TOFA (Selleckchem, S6690); dorsomorphin (Selleckchem, S7306); alisertib (Selleckchem, S1133); verdinexor (Cayman, 26171); leptomycin B (Cayman, 10004976); tipifarnib (MedchemExpress, HY-10502); methotrexate (Selleckchem, S1210); pitstop2 (Sigma, SML1169); EML425 (Selleckchem, S2977); C646 (Selleckchem, S7152); brequinar (Selleckchem, S3565); elamipretide (Selleckchem, S9803)

Chen S.Y., Lin C.C., Wu J., Chen Y., Wang Y.E., Setayeshpour Y., Mestre A, & Chi J.T. (2024). NINJ1 regulates ferroptosis via xCT antiporter interaction and CoA modulation. bioRxiv.

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Inhibitors Modulate DHFR Metabolism

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RO 48–8071 fumarate, clorgyline hydrochloride, malic enzyme inhibitor ME1 were obtained from MedChemExpress. Lometrexol and BSO were from Cayman Chemical. A922500 and brequinar were from Selleck Chemicals. Polydatin was from Santa Cruz Biotechnology. All other metabolic inhibitors used in the study were from Sigma Aldrich. Tetrahydrofolate was obtained from Sigma-Aldrich. Lentiviral short hairpin RNA clone for DHFR was from Dharmacon (sense 5’-CCTGAGAAGAATCGACCTTTA-3’). pLenti6.3/V5-DEST vector encoding DHFR and pLX304-V5-CD24 are obtained from DNASU (HsCD00940668 and HsCD00442245). Primers for HLA-A (forward 5’-GAGGAGGAAGAGCTCAGATAGA-3’ and reverse 5’-GGCAGCTGTCTCACACTTTA-3’) and all other commercially available primers for quantitative RT-PCR were obtained from Integrated DNA Technologies. FITC-conjugated anti-human CD24 antibody (SN3/ab30350) was purchased from Abcam. Anti-V5 antibody (sc-81594) and anti-β-actin antibody (AC-74/A2228) are from Santa Cruz Biotechnology and Sigma-Aldrich, respectively. CellTrace^™ CFSE Cell Proliferation Kit (C34554A) is from Life Technologies.

Boese A.C., Hwang J.S., Young I., Malin C.M., Avalos V., Kang J, & Kang S. (2022). Metabolic inhibitor screening identifies dihydrofolate reductase as an inducer of the tumor immune escape mediator CD24. Immunomedicine, 2(2), e1041.

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Antiviral Assay Protocol with Brequinar and Dipyridamole

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Brequinar (Selleck Chemicals, Cat# S6626) and dipyridamole (Sigma Aldrich Prod# D9766) were provided by Clear Creek Bio. Uridine (99% purity) was obtained from Sigma (Cat#: U3003-5G) and Alfa Aesar/Fisher (AAA1522706) for the NTP and antiviral assays, respectively.

Demarest J.F., Kienle M., Boytz R., Ayres M., Kim E.J., Patten J.J., Chung D., Gandhi V., Davey R.A., Sykes D.B., Shohdy N., Pottage JC J.r, & Kumar V.S. (2022). Brequinar and dipyridamole in combination exhibits synergistic antiviral activity against SARS-CoV-2 in vitro: Rationale for a host-acting antiviral treatment strategy for COVID-19. Antiviral Research, 206, 105403.

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