Nox2 mice

Several types of adult mice aged from 10 to 12 weeks were used,
including: WT C57b/6J (Jackson Lab), WT C57b/6N (Jackson Lab), Ser280Ala
KI,^{25 ,31 (link)} MMVV-KI,^{32 (link)} CaMKIIδ cardiac-specific and
global KO,^{33 (link),34 (link)} NOX2^−/− mice
(Jackson Lab) and Grx1-roGFP2 (targeted either to the cytosol or
mitochondria).^{27 (link)} All
the genetically modified mouse lines were bred on the C57b/6J background, except
for the C57b/6N line. See Online Methods for more details.
Cardiac ventricular myocytes were isolated using previously described
methods^{35 (link)} which were
approved by the UC Davis Institutional Animal Care and Use Committee (IACUC).
Freshly isolated myocytes were plated on laminin-coated glass cover slips for 30
min before dye loading. All experiments were performed at room temperature (RT)
(22–23°C) with pH 7.4.

SD rats were from Peking Vital River Laboratory and Centre of Experimental Animal of Peking University. C57 BL/6J (NOX2 wild type, NOX2^+/+) and NOX2^−/− mice were from Jackson Laboratories (Bar Harbor, Maine, USA). Breeding schedules were designed to achieve accurately timed-pregnancy of 14 ± 0.5 days for rats and 13 ± 0.5 days for mice. Housing, breeding and experimental use of the animals were performed in strict accordance with the National Institutes of Health guidelines.