Total 149 GBC tumor samples were stained with the PLEK2 (Proteintech), CCL2 (Proteintech) and EGFR (Santa Cruz) antibody. The staining was scored as the intensity of the positive staining (0 - negative, 1 - weak, 2 - moderate, 3 - strong) multiplied by the staining areas (0 = negative, 1 = 1 —9%, 2 = 10—39%, 3 = 40 —69%, and 4 = 70 —100%). These scores were independently determined by two pathologists.
Plek2
Manufactured by Proteintech
PLEK2 is a laboratory equipment product designed for the analysis of protein-protein interactions. It utilizes a high-throughput screening approach to identify and quantify protein-protein complexes. The core function of PLEK2 is to facilitate the study of protein-protein interactions, a fundamental aspect of biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Phosphorylated pi3k, by Cell Signaling Technology (1 mentions)
Matrix metalloproteinase mmp 2, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
Ly294002, by MedChemExpress (1 mentions)
2 protocols using plek2
1
Immunohistochemical Evaluation of PLEK2, CCL2 and EGFR in GBC
2
Regulation of Cell Proliferation and Migration via PI3K/AKT Pathway
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cell culture reagents, including culture medium, fetal bovine serum (FBS) and penicillin-streptomycin were purchased from Gibco; Thermo Fisher Scientific, Inc. The Cell Counting Kit-8 (CCK-8) and EdU Proliferation kits were purchased from Dojindo Molecular Technologies, Inc., and Guangzhou RiboBio Co., Ltd., respectively. The agonist (insulin-like growth factor 1) and inhibitor (LY 294002) of PI3K/AKT pathway were purchased from MedChemExpress, Inc. The primary antibodies used were against PLEK2 (ProteinTech Group, Inc.), N-cadherin (Cell Signaling Technology, Inc.), matrix metalloproteinase (MMP)2 (Cell Signaling Technology, Inc.), vimentin (Cell Signaling Technology, Inc.), PI3K (Cell Signaling Technology, Inc.), phosphorylated (p)-PI3K (Cell Signaling Technology, Inc.), AKT (Cell Signaling Technology, Inc.), p-AKT (Cell Signaling Technology, Inc.) and GAPDH (Jackson ImmunoResearch Labortaories, Inc.).
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