C myc

Manufactured by Takara Bio

Sourced in China

The C-Myc is a lab equipment product developed by Takara Bio. It is a protein that plays a crucial role in the regulation of gene expression and cellular processes. The C-Myc product provides researchers with a tool to study this important cellular component.

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Lab products found in correlation

β actin, by Merck Group (2 mentions) Hsp60, by Abcam (1 mentions) Image lab, by Bio-Rad (1 mentions) Igf ir, by Thermo Fisher Scientific (1 mentions) Igf 1, by Merck Group (1 mentions) Ubiquitin, by Proteintech (1 mentions) Hnrnpu, by Proteintech (1 mentions) Goat anti mouse igg h l hrp, by Affinity Biosciences (1 mentions) Gw4869, by MedChemExpress (1 mentions) β actin, by Cell Signaling Technology (1 mentions) C myc, by GeneTex (1 mentions) Flag tag (flag), by Yeasen (1 mentions) Cycloheximide (chx), by Yeasen (1 mentions) Usp16, by Merck Group (1 mentions) Plvx vector, by Takara Bio (1 mentions) C myc, by Cell Signaling Technology (1 mentions) Puromycin, by Merck Group (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) C myc, by Abcam (1 mentions) Pei 25k, by Polysciences (1 mentions)

Spelling variants of this product

Anti-c-Myc Monoclonal antibody (4 citations) Anti-c-Myc (3 citations) C-Myc Monoclonal Antibody-Agarose Beads (3 citations) PCMV-Myc-C vector (3 citations) Anti-c-Myc antibody (3 citations) C-Myc antibody (2 citations)

6 protocols using c myc

Western Blot Analysis of c-Myc and Hsp60

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Proteins for western analysis were isolated according to a protocol by Foiani (31 ). Western analysis was performed according to standard protocols. The primary antibodies for c-Myc (Clontech) and Hsp60 (Abcam) were used according to the manufacturer's protocol. Hsp60 served as a reference protein. As secondary antibody, we used a HRP-coupled antibody (Santa Cruz Biotechnology). Proteins were detected by chemiluminescence. Quantification was performed using Image Lab (BioRad).

Wanzek K., Schwindt E., Capra J.A, & Paeschke K. (2017). Mms1 binds to G-rich regions in Saccharomyces cerevisiae and influences replication and genome stability. Nucleic Acids Research, 45(13), 7796-7806.

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Antibody Profiling for Cellular Signaling

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The following antibodies were used: p IGF-IR^Y1131 (3021), pALK^Y1604 (Y664 in NPM-ALK; 3341), pAKT^S473 (4051), and Ikaros (5443) (Cell Signaling Technology, Danvers, MA); IRS-1 (ab40777), AKT (ab8805), NPM (ab52644), and MZF1 (ab64866) (Abcam, Cambridge, MA); IGF-IR (396700; Life Technologies, Grand Island, NY); pIRS-1^S639 (sc-22300; Santa Cruz Biotechnology, Santa Cruz, CA); ALK (M719501-2; Dako, Carpinteria, CA); c-Myc (631206, Clontech Laboratories, Mountain View, CA); IGF-I (05–172; Millipore, Billerica, MA); and β-actin (A-5316; Sigma).

Vishwamitra D., Curry C.V., Alkan S., Song Y.H., Gallick G.E., Kaseb A.O., Shi P, & Amin H.M. (2015). The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma. Molecular Cancer, 14, 53.

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Protein Analysis in Extracellular Vesicles

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The primary antibodies were at the dilution of 1:1000, including HNRNPU (16365–1-AP, ProteinTech Group, China), HA (51064–2-AP, ProteinTech Group, China), c-Myc (s1826, Clontech, Japan), Alix (2171S, Cell Signaling Technology, USA), CD9 (13174S, Cell Signaling Technology, USA), Ubiquitin (10201–2-AP, ProteinTech Group, China), β-actin (4970S, Cell Signaling Technology, USA). The goat anti-rabbit IgG/Alexa fluor 647 (BS-0295-G, Bioss, China) was at the 1:200 dilution. The second antibodies goat anti-rabbit IgG(H + L) HRP (FMS-Rb01, Fcmacs) and goat anti-mouse IgG (H + L) HRP (S0002, Affinity) were in 5000 diluted concentrations. GW4869 was purchased from MedChemExpress (Monmouth Junction, NJ, USA).

Tang X., Deng Z., Ding P., Qiang W., Lu Y., Gao S., Hu Y., Yang Y., Du J, & Gu C. (2022). A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. Journal of Experimental & Clinical Cancer Research : CR, 41, 85.

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Identifying USP16 and c-Myc Interactions

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Short hairpin RNA (shRNA) sequences are listed in Additional le 1: Table S1. These sequences were cloned into the pLKO.1 vector, while Flag-tagged USP16 and c-Myc were cloned into the pLVX vector (Clontech 632187). Plasmids were transfected into HEK293FT cells using PEI 25K (23966-1; Polysciences, Warrington, PA, USA) according to the manufacturer's instructions. Stable transformants of PC3 and DU145 cells were isolated in standard medium supplemented with puromycin (5 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA) for 7 days.
Reagents and primary antibodies MG132(52801ES08) and CHX (40325ES03) were purchased from Yeason (Shanghai, China). The following antibodies were used for western blotting: USP16 (A5861; Abclonal), c-Myc (GTX103436; GeneTex), β-actin (sc-47778; Santa Cruz Biotechnology). Antibodies used for immunohistochemistry: USP16 (HPA021140; Sigma-Aldrich), Ki67 (sc-15402; Santa Cruz), c-Myc (#ab32072, Abcam). Antibodies used for immunoprecipitation and immuno uorescence: Flag (#30503ES60, Yeason), USP16 (HPA021140; Sigma-Aldrich), c-Myc (13987S, Cell Signaling Technology).

Ge J., Yu W., Li J., Ma H., Wang P., Zhou Y., Wang Y., Zhang J., & Li X. (2020). USP16 Regulates Castration-Resistant Prostate Cancer Cell Proliferation by Deubiquitinating and Stablizing c-Myc.

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Profiling Oncogene Expression in Liver Cancer

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Total RNA was isolated from HuH-7, HepG2, SNU-449 and SNU-387 cells treated for 48 h using Trizol Reagent (Invitrogen; Carlsbad, CA, USA), and then reverse transcribed using Prime Script Reagent RT Kit (Takara Biotechnology Co.; Dalian, China) following the manufacturer's protocol. The PCR-primers for FOXO3a, ZEB1, CyclinD1 and v-myc avian myelocytomatosis viral oncogene homolog (c-Myc) were designed and purchased from Takara. The sequences are as follows:

FOXO3a: (forward: 5-TGCGTGCCCTACTTCAAGGATAA-3; reverse: 5-ACAGGTTGTGCCGGATGGA-3)

ZEB1: (forward: 5-CTTGAACGTCACATGACATCACATA-3; reverse: 5-TCTTGCAGTTTGGGCATTCATA-3)

CyclinD1: (forward: 5-TACCGCACAACGCACTTTC-3; reverse: 5-AAGGGCTTCAATCTGTTCCTG-3),

c-Myc: (forward: 5-GCAGCTGCTTAGACGCTGGA-3; reverse: 5-CGCAGTAGAAATACGGCTGCAC-3).

RT-PCR was performed using ABI 7900 Prism HT (Applied Biosystems Inc.; Shanghai, China) followed by melting curve analysis. FOXO3a, ZEB1, CyclinD1 and c-Myc mRNA expression was normalized to β-actin (forward: 5-TGGCACCCAGCACAATGAA-3; reverse: 5-CTAAGTCATAGTCCGCCTAGAAGCA-3).

Zhou Y., Liang C., Xue F., Chen W., Zhi X., Feng X., Bai X, & Liang T. (2015). Salinomycin decreases doxorubicin resistance in hepatocellular carcinoma cells by inhibiting the β-catenin/TCF complex association via FOXO3a activation. Oncotarget, 6(12), 10350-10365.

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Immunoblotting Antibodies Catalogue Reference

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Antibodies used in this study were ABCC2/ MRP2 (catalogue number MAB4150; Millipore, Burlington, MA), RAB11A (mouse) (catalogue number 610656; BioScience, Franklin Lakes, NJ), RAB11A (rabbit) (catalogue number ab128913; Abcam, Cambridge, MA), myosin Vb (catalogue number NBP1-87746; Novus), FLAG (catalogue number F3165; Sigma-Aldrich, St Louis, MO), UNC45A (rabbit) (catalogue number HPA039228; Merck, Kenilworth, NJ), UNC45A (mouse) (catalogue number ADI-SRA-1800-F; Enzo Life Sciences, Farmington, NY), beta-tubulin (catalogue number T4026; Merck), c-myc (catalogue number 631206; Takara, Kusatsu, Japan), ezrin (catalogue number SC58758; Santa Cruz Biotechnology, Dallas, TX), beta-actin (catalogue number A5441; Sigma-Aldrich), syntaxin-3 (catalogue number ab133750; Abcam), and munc18-2 (catalogue number ab103976; Abcam). Fluorescently labeled phalloidin (catalogue number A22284) was from Invitrogen.

Li Q., Zhou Z., Sun Y., Sun C., Klappe K, & van IJzendoorn S.C. (2022). A Functional Relationship Between UNC45A and MYO5B Connects Two Rare Diseases With Shared Enteropathy. Cellular and Molecular Gastroenterology and Hepatology, 14(2), 295-310.

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