For plasma stability, LC-MS studies were performed with an ABSciex Triple TOF 4600 (ABSciex, Darmstadt, Germany) combined with a micro-LC Eksigent (Eksigent, Darmstadt, Germany) and an autosampler set at 5 °C and a thermostated column compartment. Electrospray ionization (ESI) in negative mode was used for the MS experiments. The data acquisition method consisted of a TOF-MS full scan m/z 50–850 Da and several IDA-TOF-MS/MS (Information Dependent Acquisition) product ion scans using 40 V Collision Energy (CE) with 15 V (Collision Energy Spread) CES used for each candidate ion in each data acquisition cycle (1091). Halo C18 2.7 μm, 90 Å, 0.5 × 50 mm2 from Eksigent was used as a column and the mobile phase consisted of a gradient (A: acetonitrile/0.01% formic acid/isopropanol 80/20 v/v; B: H2O/0.01% formic acid). The elution gradient adopted started with 5% of phase B for 0.5 min, gradually increasing to 98% in the next 7.5 min. These conditions were kept constant for 0.5 min, and then the initial conditions (95% solvent B, 5% solvent A) were restored within 0.1 min to re-equilibrate the column for 1.5 min for the next injection (flow rate 55 µL/min). The data acquisition was carried out with MultiQuant 3.0.2 and PeakView 2.1 from AB SCIEX (ABSciex, Darmstadt, Germany).
Triple tof 4600
Manufactured by AB Sciex
Sourced in United States, Japan, Canada
The Triple TOF 4600 is a high-resolution, accurate-mass mass spectrometer system designed for advanced analytical applications. It combines the performance of a triple quadrupole with the high resolution and mass accuracy of a time-of-flight (TOF) analyzer. The system is capable of performing both high-sensitivity quantitative analysis and high-resolution qualitative analysis.
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Lab products found in correlation
Avance 400 mhz spectrometer, by Bruker (3 mentions)
Nexera uhplc system, by Shimadzu (2 mentions)
Ft ir 4200 spectrometer, by Jasco (2 mentions)
Jmn ecz400s, by JEOL (2 mentions)
F 4600 fluorescence spectrophotometer, by Hitachi (2 mentions)
Lsm 710, by Zeiss (2 mentions)
Analyst tf 1, by AB Sciex (2 mentions)
Peakview 2, by AB Sciex (1 mentions)
Multiquant 3, by AB Sciex (1 mentions)
Xtalab synergy s mo system, by Rigaku (1 mentions)
V 650 spectrophotometer, by Jasco (1 mentions)
Nexera uhplc lc 30a, by Shimadzu (1 mentions)
Ascend 400, by Bruker (1 mentions)
Drx 600 spectrometer, by Bruker (1 mentions)
Luna omega c 18 column, by Shimadzu (1 mentions)
Alkaline phosphatase, by Takara Bio (1 mentions)
Phosphodiesterase 1, by Merck Group (1 mentions)
Nuclease p1, by Merck Group (1 mentions)
Luna omega c18 column, by Phenomenex (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
29 protocols using triple tof 4600
1
Plasma Stability Assessment by LC-MS
2
Photochemical Synthesis and Characterization
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All the reagents and solvents were commercially available and used as received. UV light (310 nm) was irradiated using Techno Sigma LED PER-AMP (Okayama, Japan). The 1H-NMR spectra were measured on a JEOL JMN-ECZ400S (Tokyo, Japan) at 400 MHz with tetramethylsilane as an internal standard. The 13C-NMR spectra were measured on a JEOL JMN-ECZ400S (Tokyo, Japan) at 100 MHz, and assignments of 13C-NMR spectra were performed via DEPT experiments. Absorption spectra were recorded on a JASCO V-650 spectrophotometer (Tokyo, Japan). The IR spectra were recorded on a JASCO FT/IR-4200 spectrometer (Tokyo, Japan). The high-resolution mass spectra were measured on an AB SCIEX Triple TOF 4600 (Tokyo, Japan). Diffraction data were collected at 93 K under a cold N2-gas stream on a Rigaku XtaLAB Synergy-S/Mo system (λ=0.71073 Å (Mo-Kα), Tokyo, Japan). The integrated data were analyzed by using a Yadokari-XG software package [24 (link)]. The structures were solved with the ShelXT structure solution program [25 (link)] using Intrinsic Phasing and refined with the ShelXL refinement package [26 (link)] using least-squares minimization. Anisotropic refinement was performed for all non-hydrogen atoms, and all the hydrogen atoms were put in calculated positions. The geometrical optimization was carried out at the CAM-B3LYP/6-31G(d,p) level of theory implemented on the Gaussian 09 package [27 ].
3
Characterization of CPP-BoNTA Proteins by MS
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Mass spectrometry analyses of CPP-BoNTA proteins ( Prepare 10–50 μg of protein samples with a purity of more than 90%. Use AB SCIEX TripleTOF™ 4600 coupled directly to a Nexera UHPLC LC-30A(SHIMADZU) with a 100 mm × 2.15 μm ACE C4 column for all LC-MS/MS analyses. Set the mobile phase A to be H2O with 0.1 FA, the mobile phase B to be CH3CN with 0.1 FA, and the flow rate to be 0.4 mL/min. Use Peakviewer software for database searching and spectral interpretation. MS analysis of the molecular weight of CPP-BoNTA proteins
4
NMR and Mass Spectroscopy Protocol
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All the reagents and solvents were commercially available and used as received. The 1H-NMR spectra were measured on a Bruker Ascend-400 (Bruker, Billerica, MA, USA) at 400 MHz with TMS as an internal standard. The 13C-NMR spectra were measured on a Bruker Ascend-400 at 100 MHz, and assignments of 13C-NMR spectra were performed by DEPT experiments. The high resolution mass spectra were measured on a AB SCIEX Triple TOF 4600 (AB Sciex, Framingham, MA, USA).
5
Characterization of Organic Compounds
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All commercially available chemicals were directly used with no further purification after being purchased from Sigma Aldrich. The micro melting point apparatus (SGW X-4B, Shanghai, China) was used to test all the melting points. The DRX-600 spectrometer (Bruker, Rhenistetten-Forchheim, Germany) was used to record the 1H NMR and 13C NMR spectra. The AB SCIEX Triple-TOF 4600 (USA) was used to conduct the High-Resolution Mass Spectrometry (HRMS) analysis.
6
Comprehensive UHPLC-QTOF Analysis of Extracts
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Extracts were analyzed using a NEXERA UHPLC system (Shimadzu, Tokyo, Japan) and the Luna® Omega C-18 column (Shimadzu, Tokyo, Japan). The linear gradient for separation purposes consisted of water (A) and acetonitrile (B), both with 0.1% formic acid. B eluent was held at 5% for 0.5 min and was allowed to reach 17.5% at 5 min; then, it was increased to 45% in 5 min and held at this % for the other 2 min. Finally, it was augmented to 95% in 1 min, and following another min at 95%, initial conditions were restored in 1 min, allowing for the system to re-equilibrate for 1 min. The flow rate was 0.5 mL/min. The injection volume was 2.0 μL. MS analysis was performed using the AB SCIEX Triple TOF® 4600 (AB Sciex, Concord, ON, Canada), equipped with a DuoSprayTM ion source, operating in negative ESI mode. A full scan TOF survey (170–1700 Da) and eight IDA (information-dependent acquisition) experiments were performed using the following parameters: curtain gas 35 psi; nebulizer gas 60 psi; heated gas 60 psi; ion spray voltage −4.5 kV; interface heater temperature 600 °C; declustering potential −80V; collision energy −45 ± 10 V. The instrument was controlled by Analyst® TF 1.7.1 software. Data processing was carried out by PeakView® software version 2.2.
7
Small RNA Nucleoside Profiling
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1 μg of small RNAs (18–24nt) or miR-21-5p was degraded with 0.2 unit nuclease P1 (Sigma-Aldrich) at 50°C for 3 h in 50 mM NH4OAc (pH5.3). For further digestion into mononucleosides, 0.04 unit phosphodiesterase I (Sigma-Aldrich) was added and incubated at 37°C for another 2 h (pH 8.8). Finally, small RNAs or miR-21-5p was incubated with 2 unit alkaline phosphatase (Takara) for 2 h at 37°C. The proteins in nucleoside digests were removed by Amicon® Ultra 3K device with centrifugation in 12 000 × g for 30 min. Subsequently, the metal ion in hydrolysates was removed by HyperSep™ Hypercarb™ SPE (ThermoFisher). Hydrolysates were-suspended with 80% acetonitrile (containing 0.1% formic acid) for LC-MS/MS analysis. These nucleosides were subjected to LC–MS/MS analysis using AB SCIEX TripleTOF® 4600 in positive ion mode.
8
Identification of Electron Mediators in K. quasipneumoniae MFCs
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To determine the electron mediators secreted by K. quasipneumoniae sp. 203 in MFCs, high performance liquid chromatography mass spectrometry (HPLC–MS, AB SCIEX 4600 Triple TOF, Co, Ltd, USA) was used for analysis. The anolyte samples were pretreated and the method was modified as described by Islam et al. [35 (link)]. The HPLC program parameters were similar to those described by Wang et al. [36 (link)]. The column temperature was kept at 35 °C. MS data was collected via an AB SCIEX TripleTOF 4600 mass spectrometer. The mass spectrometry parameters were: in ESI + mode, the ionization energy was set to 10 V, the cluster potential was set at 70 V, the electron multiplier voltage was 4500 V, and the mass-to-charge ratio (m/z) was selectively monitored from 50 to 1200.
9
UHPLC-Q-TOF MS for Compound Analysis
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A Shimadzu NEXERA UHPLC system (Shimadzu, Japan) was used with a Luna® Omega C18 column (1.6 μm particle size, 50 × 2.1 mm i.d., Phenomenex, Torrance, CA, USA).
Separation was achieved with a linear gradient of water (A) and acetonitrile (B), both with 0.1% formic acid. Gradient conditions were as follows: 0–5 min, linear from 5% to 55% B; 5–10 min, linear from 55% to 75% B; 10–11 min, linear from 75% to 95% B; 11–13 min, isocratic 95% B. Then, the starting conditions were restored and the column was allowed to re-equilibrate for 1 min. The total run time was 14 min, with a flow rate of 0.5 mL min−1 and an injection volume of 2.0 μL.
MS analysis was performed using a hybrid QqTOF MS instrument, the AB SCIEX TripleTOF® 4600 (AB Sciex, Concord, ON, Canada), equipped with a DuoSprayTM ion source (consisting of both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) probes), which was operated in positive and negative ionization modes. The instrument was controlled by Analyst® TF 1.7 software, while data processing was carried out using PeakView® software version 2.2.
Separation was achieved with a linear gradient of water (A) and acetonitrile (B), both with 0.1% formic acid. Gradient conditions were as follows: 0–5 min, linear from 5% to 55% B; 5–10 min, linear from 55% to 75% B; 10–11 min, linear from 75% to 95% B; 11–13 min, isocratic 95% B. Then, the starting conditions were restored and the column was allowed to re-equilibrate for 1 min. The total run time was 14 min, with a flow rate of 0.5 mL min−1 and an injection volume of 2.0 μL.
MS analysis was performed using a hybrid QqTOF MS instrument, the AB SCIEX TripleTOF® 4600 (AB Sciex, Concord, ON, Canada), equipped with a DuoSprayTM ion source (consisting of both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) probes), which was operated in positive and negative ionization modes. The instrument was controlled by Analyst® TF 1.7 software, while data processing was carried out using PeakView® software version 2.2.
10
Comparative Peptide Mapping of Insulin Aspart
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In order to demonstrate that the disulphide linkage in BGL-ASP batches were highly similar to reference insulin aspart, a comparative peptide mapping analysis was performed wherein insulin aspart was digested with V8/Endoproteinase GluC (Cat no: P2922, Sigma-Aldrich) enzyme and the mixture generated was analyzed under reducing and non-reducing conditions using Liquid Chromatography-Electron Spray Ionization-Mass Spectrometry/Mass Spectrometry (LC–ESI–MS/MS). The reduction reaction was set up in the presence of Dithiothreitol (DTT) (Cat No: 10197777001, Sigma-Aldrich). The comparison between the theoretical and generated molecular weights for the peptides, assisted in demonstrating the presence of the native disulphide linkages. The untreated insulin aspart mass was also determined and compared with the innovator product. The column with the dimensions C18, 150 × 2.1 mm, 3 μm, (Cat No: TA12S05-15Q1PTH, YMC) was used for LC–MS based analysis. The detection wavelength was set at 215 nm, flow rate of 0.2 ml/min. The AB SCIEX Triple TOF 4600 was set to mass range of m/z 200–2000 Da, source temperature of 450 °C and collision energy of 35 V.
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