Rna prep with enrichment l tagmentation kit

Manufactured by Illumina

The RNA Prep with Enrichment (L) Tagmentation Kit is a Illumina lab equipment product designed for sample preparation of RNA. It enables the preparation of RNA samples for sequencing analysis.

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Lab products found in correlation

Close spelling variants of this product

RNA Prep with Enrichment kit (5 citations) RNA Prep with Enrichment (2 citations) RNA Prep with Enrichment (L) Tagmentation kit with Respiratory Virus Oligo Panel v2 (2 citations)

5 protocols using rna prep with enrichment l tagmentation kit

SARS-CoV-2 Whole Genome Sequencing

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SARS-CoV-2 whole-genome sequencing was performed by using QIAseq DIRECT SARS-CoV-2 Kit (QIAGEN). The quality of paired-end reads obtained from MiSeq sequencing was analyzed by using Qiagen CLC Genomics Workbench 22.0.1 and the Identify ARTIC V3 SARS-CoV-2 Low Frequency and Shared Variants (Illumina) workflow was used in genetic variant analyses. Nucleotide sequences were aligned using MAFFT v7.471, and the mutations were analyzed using nextclade (https://clades.nextstrain.org). Pan-viral target hybridization enrichment sequencing was performed by using RNA Prep with Enrichment (L) Tagmentation Kit (Illumina) and Comprehensive Viral Research Panel (Twist Biosciences).

Wang Y., Lenoch J., Kohler D., DeLiberto T.J., Tang C., Li T., Tao Y.J., Guan M., Compton S., Zeiss C., Hang J, & Wan X.F. (2022). SARS-CoV-2 exposure in Norway rats (Rattus norvegicus) from New York City. bioRxiv.

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Illumina Respiratory Virus Enrichment

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Hybridization capture was performed with an Illumina RNA Prep with Enrichment (L) Tagmentation kit (Illumina #20040537), IDT for Illumina DNA/RNA UD Indexes (Illumina #20027213), and Respiratory Virus Oligo Panel v2 (Illumina #20044311). Tagmented cDNAs were amplified to add indexes and adapters, and the resulting libraries normalized using Qubit for 1- or 3-plex enrichment by hybridization to sequence-specific biotinylated probes. The captured sequences were washed, eluted, and amplified to generate copies of the enriched libraries, which were further amplified and cleaned with AMPure XP Beads, normalized, and pooled for 1 × 101 or 2 × 151 cycle sequencing on an Illumina NovaSeq or NextSeq2000 instrument.

Lieber C.M., Kang H.J., Aggarwal M., Lieberman N.A., Sobolik E.B., Yoon J.J., Natchus M.G., Cox R.M., Greninger A.L, & Plemper R.K. (2024). Influenza A virus resistance to 4’-fluorouridine coincides with viral attenuation in vitro and in vivo. PLOS Pathogens, 20(2), e1011993.

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FFPE Exome RNA Sequencing Protocol

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Genomic DNA and RNA was extracted from 5 μm sections of formalin fixed paraffin embedded tissue (FFPE) sections mounted on slides using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany), RNeasy FFPE Tissue Kit (Qiagen), or AllPrep DNA/RNA FFPE Kit (Qiagen). DNA was set aside for methylation analysis as described below. Next-generation sequencing (NGS) was performed using commercial TruSeq RNA Exome panel (Illumina). Exome RNA sequencing libraries were prepared with 100 ng tumor RNA using the Illumina RNA Prep with Enrichment (L) Tagmentation kit (Illumina) with Exome Probe Panel (Illumina). Final enriched libraries are sequenced on NextSeq 550DX or NovaSeq 6000 (Illumina).

Andreiuolo F., Ferrone C.K., Rajan S., Perry A., Guney E., Cham E., Giannini C., Toland A., Willard N., de Souza A.S., Dazelle K., Chung H.J., Singh O., Conway K., Coley N., Dampier C., Abdullaev Z., Pratt D., Cimino P.J., Quezado M, & Aldape K. (2024). Molecular and clinicopathologic characteristics of CNS embryonal tumors with BRD4::LEUTX fusion. Acta Neuropathologica Communications, 12, 42.

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SARS-CoV-2 Genomic Sequencing From Clinical Samples

Cited 1 time

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The Ohio State Applied Microbiology Services Laboratory attempted genomic sequencing on all positive samples with a Ct value of 33 or lower (n = 86 samples). RNA was reverse transcribed into cDNA and PCR amplified using the ARTIC v 4.1 SARS-CoV-2 primer panel and NEBNext^® FS Library Prep Kit for Illumina^® (New England Biolabs, Ipswich MA) per manufacturers protocol instructions. Illumina sequencing libraries were prepared using RNA Prep with Enrichment (L) Tagmentation Kit (Illumina, San Diego, CA) per manufacturers protocol with unique dual indexes (Illumina). Libraries were pooled and quantified using ProNex NGS Library Quant Kit (NG1201, Promega Co. Madison, WI). Sequencing with NextSeq 2000 (Illumina) and assembly by DRAGEN (Illumina) were performed as previously described^{3 (link)}.

McBride D., Garushyants S., Franks J., Magee A., Overend S., Huey D., Williams A., Faith S., Kandeil A., Trifkovic S., Miller L., Jeevan T., Patel A., Nolting J., Tonkovich M., Genders J.T., Montoney A., Kasnyik K., Linder T., Bevins S., Lenoch J., Chandler J., DeLiberto T., Koonin E., Suchard M., Lemey P., Webby R., Nelson M, & Bowman A. (2023). Accelerated evolution of SARS-CoV-2 in free-ranging white-tailed deer. Research Square.

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Genomic Sequencing of SARS-CoV-2 Positive Samples

Cited 1 time

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The Ohio State Applied Microbiology Services Laboratory attempted genomic sequencing on all positive samples with a Ct value of 33 or lower (n = 86 samples). RNA was reverse transcribed into cDNA and PCR amplified using the ARTIC v 4.1 SARS-CoV-2 primer panel (Integrated DNA Technologies, Inc. cat #10011442) and NEBNext® FS Library Prep Kit for Illumina® (New England Biolabs, Ipswich MA) per manufacturers protocol instructions. Illumina sequencing libraries were prepared using RNA Prep with Enrichment (L) Tagmentation Kit (Illumina, San Diego, CA) per manufacturers protocol with unique dual indexes (Illumina). Libraries were pooled and quantified using ProNex NGS Library Quant Kit (NG1201, Promega Co. Madison, WI). Sequencing with NextSeq 2000 (Illumina) and assembly by DRAGEN COVID Lineage app v.3.5.6 (Illumina) were performed as previously described^{11 (link)}.

McBride D.S., Garushyants S.K., Franks J., Magee A.F., Overend S.H., Huey D., Williams A.M., Faith S.A., Kandeil A., Trifkovic S., Miller L., Jeevan T., Patel A., Nolting J.M., Tonkovich M.J., Genders J.T., Montoney A.J., Kasnyik K., Linder T.J., Bevins S.N., Lenoch J.B., Chandler J.C., DeLiberto T.J., Koonin E.V., Suchard M.A., Lemey P., Webby R.J., Nelson M.I, & Bowman A.S. (2023). Accelerated evolution of SARS-CoV-2 in free-ranging white-tailed deer. Nature Communications, 14, 5105.

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