The levels of 3-nitro-L-tyrosine (3′-NT), 4-HNE, and protein carbonyl were checked by enzyme-linked immunosorbent assay (ELISA) using commercial kits following the manufacturer's instruction. The thiobarbituric acid reactive substances (TBARS) and GSH/GSSG ratio were assessed by TBARS assay kit (Abnova, USA) and GSH/GSSG assay kit (Abcam), respectively. The levels of iron, Fe2+, and MDA were measured by the related kit (Abcam, USA).
Gsh gssg assay kit
Manufactured by Abcam
Sourced in United States, China
The GSH/GSSG assay kit is a quantitative colorimetric assay designed to measure the levels of reduced glutathione (GSH) and oxidized glutathione (GSSG) in biological samples. The kit provides a simple and reliable method for the simultaneous detection of both GSH and GSSG, allowing the evaluation of the cellular redox state.
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Lab products found in correlation
2 protocols using gsh gssg assay kit
1
Oxidative Stress Biomarkers Analysis
2
Oxidative Stress Biomarkers in Kidney
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The contents of malondialdehyde (MDA), superoxide dismutase (SOD), and iron and the glutathione/oxidized glutathione (GSH/GSSG) ratio in kidney tissues and podocytes were detected using MDA (thiobarbituric acid method) and SOD assay kits (hydroxylamine method; Jiancheng Bioengineering Institute, Nanjing, China), an Iron content assay Kit (ferrozine microplate method; Leagene Biotechnology, Beijing, China), and a GSH/GSSG assay kit (fluorometric—green; ab205811, Abcam), respectively, following the corresponding kit instructions.
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