Rabbit anti cyclooxygenase cox 2

Western blot analyses were performed as previously described [30 (link)]. Protein samples obtained from tissue or BV2 microglia homogenates were separated on 8–12% sodium dodecyl sulfate-polyacrylamide gels, transferred to Immobilon-P membranes (Millipore), and probed overnight at 4 °C with primary antibodies including rabbit anti-sEH (1:1000) and rabbit anti-P65 (1:1000) from Santa Cruz; rabbit anti-iNOS (1:1000) and rabbit anti-cyclooxygenase (COX-2 1:1000) from Cayman; rabbit anti-cCP-3 (1:1000), rabbit anti-p-P38 (1:1000), rabbit anti-P38 (1:2000), rabbit anti-p-C-Jun N-terminal kinase (JNK, Thr183/Tyr185, 1:1000), rabbit anti-JNK (1:2000), rabbit anti-p-extracellular signal-regulated kinase p44/42 (ERK p44/42; Thr202/Tyr204, 1:1000), rabbit anti-ERK (1:2000), and rabbit anti-p-P65(1:1000) from Cell Signaling (Danvers, MA, USA); and mouse anti-β-actin (1:10,000, Sigma-Aldrich). Protein band intensities were quantified using ImageJ software and were normalized to the corresponding β-actin intensity.