Hemozoin

Manufactured by Merck Group

Hemozoin is a laboratory product developed by Merck Group. It is a crystalline pigment found in the blood of malaria-infected individuals. Hemozoin is a by-product of the malaria parasite's digestion of hemoglobin. The core function of Hemozoin is to serve as a marker for the diagnosis and study of malaria.

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Lab products found in correlation

Tumor necrosis factor (tnf), by Thermo Fisher Scientific (1 mentions) Opti mem reduced serum medium, by Thermo Fisher Scientific (1 mentions) 48 well plate, by Thermo Fisher Scientific (1 mentions) Haoec, by PromoCell (1 mentions) Gelatin, by Merck Group (1 mentions) Cytotoxicity detection kit, by Merck Group (1 mentions) Hemozoin, by InvivoGen (1 mentions) Endothelial cell growth medium mv2, by PromoCell (1 mentions)

2 protocols using hemozoin

Macrophage Response to NET Stimulation

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Monocytes were isolated by magnetic CD14 positive selection (130-050-201, Miltenyi Biotec) and differentiated for 7 days into macrophages in RPMI 1640 containing penicillin/streptomycin, glutamine and 5 ng/ml human MCSF. At the day of the experiment, 3x10⁶ neutrophils were stimulated for 4 h with 50 nM PMA. The resulting NETs were washed three times with PBS, harvested by scraping and sonicated. The NET concentration was determined by Picogreen assay. Macrophages were stimulated for 12 h with 1 μg/ml isolated NETs, 100 μg/ml hemozoin (Sigma), 2 ng/ml TNF (Peprotech) or 100 ng/ml LPS from Salmonella (Enzo Life Sciences).

Knackstedt S.L., Georgiadou A., Apel F., Abu-Abed U., Moxon C.A., Cunnington A.J., Raupach B., Cunningham D., Langhorne J., Krüger R., Barrera V., Harding S.P., Berg A., Patel S., Otterdal K., Mordmüller B., Schwarzer E., Brinkmann V., Zychlinsky A, & Amulic B. (2019). Neutrophil extracellular traps drive inflammatory pathogenesis in malaria. Science immunology, 4(40), eaaw0336.

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Endothelial Cell Response to Hemozoin

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Primary Human Aortic Endothelial cells (HAoECs) were obtained from PromoCell GmbH, Heidelberg, Germany. The cells were cultured in Endothelial Cell Growth Medium MV2 (PromoCell), passaged by treatment with Trypsin/EDTA (0.04%/0.03%) and grown in 48-well plates (Thermo Scientific, Roskilde, Denmark) coated with 1% gelatin (Sigma, St Louis, MO). In the experiments the cells were cultured with and without recombinant human (rh)IL-27 (100 ng/mL; R&D Systems, Minneapolis, MN) in Opti-MEM reduced serum medium (Gibco, Grand Island, NY) supplemented with 5% FBS for 1 h before stimulated with different concentrations of chemically synthesized hemozoin (Invivogen, San Diego, CA) for 22 h. For evaluation of possible cell toxicity different concentration of hemozoin was tested in HAoEC cultures using Cytotoxicity Detection Kit from Sigma Aldrich (St. Louis, MO).

Otterdal K., Berg A., Michelsen A.E., Yndestad A., Patel S., Gregersen I., Halvorsen B., Ueland T., Langeland N, & Aukrust P. (2021). IL-18 and IL-18 binding protein are related to disease severity and parasitemia during falciparum malaria. BMC Infectious Diseases, 21, 1073.

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