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T9026

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

T9026 is a laboratory centrifuge. It is designed for the separation of cellular components, proteins, and other biomolecules from complex biological samples through the process of centrifugation.

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Lab products found in correlation

Anti tom20, by Santa Cruz Biotechnology (1 mentions) Anti ck19, by Abcam (1 mentions) Ab68153, by Abcam (1 mentions) Ab76315, by Abcam (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Mbd3l2, by Abcam (1 mentions) β2 ar r11e1, by Santa Cruz Biotechnology (1 mentions) Ab1791, by Abcam (1 mentions) Trans blot turbo, by Bio-Rad (1 mentions) H6908, by Merck Group (1 mentions) Sc 56, by Santa Cruz Biotechnology (1 mentions)

4 protocols using t9026

1

Multicolor Immunolabeling Protocol

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The following primary antibodies and dyes were used: anti-alpha tubulin (Sigma, T9026, used at 1:10000), anti-Tom20 (Santa Cruz, 11415, used at 1:300), anti-CK19 (Abcam, 52625, used at 1:1000 for IF and 1:100 for IHC), phalloidin-tetramethylrhodamine B isothiocyanate (Sigma, P1951, used at 1:500), DAPI (Sigma, D9542, used at 1:500), and WGA-647 (Molecular probes, W32466, used at 0.1 mg/ml). For immunofluorescence, the following secondary antibodies were used: anti-rabbit FITC (Invitrogen, F2765, used at 1/500), anti-mouse AF405 (Invitrogen, A31553, used at 1/500), and anti-mouse AF647 (Life technologies, A21236, used at 1/500). Tissue sections were mounted in VectaShield containing added DAPI (Vector Laboratories, H-1200).
Latario C.J., Schoenfeld L.W., Howarth C.L., Pickrell L.E., Begum F., Fischer D.A., Grbovic-Huezo O., Leach S.D., Sanchez Y., Smith K.D, & Higgs H.N. (2020). Tumor microtubes connect pancreatic cancer cells in an Arp2/3 complex-dependent manner. Molecular Biology of the Cell, 31(12), 1259-1272.
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2

Immunoblotting Analysis of Cell Signaling Pathways

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Experimental cells were harvested and lysed with modified RIPA buffer (50 mM Tris-HCl [pH 7.4], 150 mM sodium chloride, 1 mM EDTA, 1% NP40, 0.25% sodium deoxycholate, 1 mM dithiothreitol, 10 mM NaF, 1 mM PMSF, 1 μg/mL aprotinin and 1 μg/mL leupeptin). Lysates were resolved on SDS-containing 10% polyacrylamide gel, transferred to PVDF membranes, and probed with specific antibodies as follows: α-tubulin (MilliporeSigma, T9026), CEBPD (Santa Cruz Biotechnology, sc-365546), phospho STAT3 (Y705) (Abcam, ab76315), STAT3 (Abcam, ab68153), MCL1 (Abcam, ab32087), BCL-2 (GeneTex, GTX61005), and BCL-XL (GeneTex, GTX105661). Enhanced chemiluminescence was purchased from Amersham Life Sciences Inc.
Wang W.J., Lai H.Y., Zhang F., Shen W.J., Chu P.Y., Liang H.Y., Liu Y.B, & Wang J.M. (2021). MCL1 participates in leptin-promoted mitochondrial fusion and contributes to drug resistance in gallbladder cancer. JCI Insight, 6(15), e135438.
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3

Antibody Panel for Protein Analysis

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The following antibodies were used: α-Tubulin (DM1A), Sigma-Aldrich T9026; β2-AR (R11E1), Santa Cruz Biotechnology (Dallas, TX, USA) sc-81577, lot#G1117; BRD2, Bethyl Laboratories (Montgomery, TX, USA) A302-582A; BRD3, Bethyl A302-368A; BRD4, Bethyl A301-985A50; GAPDH, Thermo Fisher Scientific TAB1001; Histone H3, Abcam (Cambridge, UK) ab1791; and MBD3L2, Abcam ab107999, lot#GR126890-1; rabbit monoclonal antibody against DUX4 (E14-3) was produced in collaboration with Epitomics (Burlingame, CA, USA) and is described elsewhere [25 (link)].
Campbell A.E., Oliva J., Yates M.P., Zhong J.W., Shadle S.C., Snider L., Singh N., Tai S., Hiramuki Y., Tawil R., van der Maarel S.M., Tapscott S.J, & Sverdrup F.M. (2017). BET bromodomain inhibitors and agonists of the beta-2 adrenergic receptor identified in screens for compounds that inhibit DUX4 expression in FSHD muscle cells. Skeletal Muscle, 7, 16.
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4

Western Blot Analysis of HA-tagged Proteins

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Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes by Trans Blot Turbo (Bio-Rad). HA-tagged constructs were identified using a rabbit anti-HA antibody (Sigma, H6908, 1:10,000) and goat anti-rabbit horseradish peroxidase (Jackson ImmunoResearch, 115-035-003, 1:5000) as the secondary antibody. Equal loading was confirmed using a mouse anti-tubulin (Sigma, T9026, 1:10,000) or mouse anti-PCNA (Santa Cruz, SC-56, 1:500) antibody and goat anti-mouse horseradish peroxidase (Jackson ImmunoResearch, 111-035-003, 1:5000) as the secondary antibody. Detection was performed using the ECL detection system. Uncropped and unprocessed scans are provided in the Source Data file.
Zelnik I.D., Mestre B., Weinstein J.J., Dingjan T., Izrailov S., Ben-Dor S., Fleishman S.J, & Futerman A.H. (2023). Computational design and molecular dynamics simulations suggest the mode of substrate binding in ceramide synthases. Nature Communications, 14, 2330.
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