Raman spectroscopy was recorded via a home assembled Raman instrument equipped with a spectrograph (Kymera 328i) (ANDOR, Belfast, Northern Ireland), a microscope (Nikon eclipse Ti), and a 50x air objective with LEICA N.A. 0.75. For the preparation of the samples, the HA/graphite hybrid material pellets were coated and the HA solution was dropped onto Si wafers. An excitation laser wavelength of 532 nm and a thermoelectrically cooled Electron Multiplying charge-coupled device (EMCCD) camera (−60 °C, newton, ANDOR) were employed for the measurements. The spectra were acquired with a laser power of 31.4 mW and an integration time of 10 s on Andersolis software, in six randomly selected areas of the dry samples. The six spectra for each sample were averaged, smoothed, and baseline corrected.
Kymera 328i
Manufactured by Oxford Instruments
Sourced in United Kingdom
The Kymera-328i is a compact and versatile spectrograph designed for a wide range of applications in scientific research and industrial analysis. It features a high-resolution Czerny-Turner optical design and is capable of handling a variety of input wavelengths and light sources. The Kymera-328i provides accurate spectral data and reliable performance, making it a valuable tool for laboratory and research settings.
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Lab products found in correlation
Sr opt 8019, by Oxford Instruments (2 mentions)
Eclipse ti, by Leica (1 mentions)
P 525, by Physik Instrumente (1 mentions)
Idus 420, by Oxford Instruments (1 mentions)
Pma182, by PicoQuant (1 mentions)
Idus 416, by Oxford Instruments (1 mentions)
Ldm56, by Thorlabs (1 mentions)
Tecnai f20 tem, by Thermo Fisher Scientific (1 mentions)
Lambda 750, by PerkinElmer (1 mentions)
6 protocols using kymera 328i
1
Raman Spectroscopic Analysis of HA/Graphite Hybrids
2
Time-Resolved Photoluminescence Spectroscopy
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Time‐resolved PL spectroscopy and imaging measurements were performed by a home‐build system, which mainly includes fs pulsed laser, an inverted fluorescence microscope, and a spectrometer integrated with Time‐Correlated Single Photon Counting (TCSPC) detection. The excitation laser pulses were generated from a wavelength‐tunable femtosecond oscillator (Coherent Chameleon) with 200 fs pulse width and a repetition rate of 80 MHz. The excitation laser was introduced into the microscopy (Olympus IX71) and focused on the sample via an objective lens (60×). The optical image was taken by two Scientific CMOS cameras (PCO.panda 4.2 & Sony Starvis IMX226). Short‐pass filters (for two‐photon excitation) or long‐pass filters (for one‐photon excitation) were placed in the detection path to cut off the excitation laser before measuring. The sample was placed on a 3D nano‐translation stage (Physik Instrumente, P‐525) for varying the sample position precisely. The collected emission was further imported into a spectrometer (Andor Kymera‐328i) with two output ports. One port was connected with a charge‐coupled device (CCD) camera (Andor iDus420) for the spectra measurement. The other one was integrated with a photomultiplier detector (PicoQuant PMA182) which was associated with TCSPC (HydraHarp 400) for transient PL measurement.
3
Ultrafast Laser-based Tip-enhanced Raman Spectroscopy
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The ultrafast laser system used in the current work is a Ti:Sapphire oscillator (Element™ 2, Newport Spectra-Physics) which produces laser pulses of ~6 fs duration with a bandwidth spanning from 650 nm to 1050 nm at a repetition rate of ~80 MHz. Probe pulses centred at ~728 nm with a duration of ~500 fs were generated by narrowband filtering (Ultra Narrow Bandpass Filter 728.1/1.5, AHF) of the broadband ~6 fs long laser pulses. Pump pulses (~750–805 nm) and Stokes pulses (~805–920 nm) were also generated by bandpass filtering of the broadband laser pulses. Two precise (resolution ~0.1 μm) delay stages were used to control the delay time τ12 between the pump and Stokes pulses and τ23 between the Stokes and probe pulses. An achromatic lens (diameter: 50 mm; focusing length: 75 mm) was mounted inside the UHV chamber to focus the laser beams onto the apex of the Au tip. The TERS signal was collected through the same achromatic lens and then focused onto the entrance slit of a spectrometer (Kymera 328i, ANDOR) and detected by a thermoelectrically cooled charge coupled device (iDus 416, ANDOR). TERS experiments were also performed with CW excitation by using a Helium-Neon (He-Ne) CW laser (HNL150L, Thorlabs) centred at ~633 nm.
4
Integrating Sphere Optical Characterization
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An optical
fiber (Andor SR-OPT-8019) leads from the sphere to a grating spectrograph
(Andor Kymera-328i) and detectors (Andor iDus 420 and iDus InGaAs
1.7). Immediately in front of the optical fiber port is a baffle,
also coated with barium sulfate, preventing direct illumination of
the optical fiber, and one-bounce illumination of the optic fiber.
This arrangement sets geometric conditions on the size and placement
of the baffle with respect to the size of the LSC (SISection 1.2 ). The laser (Thorlabs L405G1, profile
and stability details in SISection 1.3 ) is coupled directly to the sphere and mounted on a temperature-controlled
stage (Thorlabs LDM56). Coupling optics were supplied by Thorlabs
and modified in-house to fit the ports of the integrating sphere.
fiber (Andor SR-OPT-8019) leads from the sphere to a grating spectrograph
(Andor Kymera-328i) and detectors (Andor iDus 420 and iDus InGaAs
1.7). Immediately in front of the optical fiber port is a baffle,
also coated with barium sulfate, preventing direct illumination of
the optical fiber, and one-bounce illumination of the optic fiber.
This arrangement sets geometric conditions on the size and placement
of the baffle with respect to the size of the LSC (SI
and stability details in SI
stage (Thorlabs LDM56). Coupling optics were supplied by Thorlabs
and modified in-house to fit the ports of the integrating sphere.
5
Characterization of Nanoplatelets by TEM and PL
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Bright-field transmission electron microscopy was performed with a FEI Tecnai F20 TEM at 200 kV operating voltage. Samples were prepared by deposition of diluted nanocrystals in hexane onto carboncoated Cu grids (Agar AGS160).
Photoluminescence Quantum Efficiency (PLQE)
Photoluminescence quantum efficiency was performed on a home-built measurement setup consisting of an integrating sphere (Labsphere 4P-GPS-053-SL), collection fibre (Andor SR-OPT8019), spectrograph (Andor Kymera-328i) and detector (Andor iDus 420). The setup was calibrated for spectral sensitivity with a NIST-traceable quartz-tungsten-halogen lamp (Newport 63967-200QC-OA).
Excitation was performed with a 520 nm temperature-controlled diode laser (Thorlabs). PLQE values were determined via the integrating sphere method. 125 Absorption Spectroscopy
Linear absorption spectra of colloidal nanoplatelets solutions, placed in a 1 mm path length cuvette (Hellma), were measured using a commercial PerkinElmer Lambda 750 UV-vis-NIR setup equipped with a 10 cm integrating sphere module attachment. A Xe lamp was used as an excitation source, and all measurements were performed under standard ambient conditions. In order to collect the scattered light, the sample cuvette was placed on the front window of the sphere. The spectra were measured simultaneously with the solvent hexane to correct for its absorption.
Photoluminescence Quantum Efficiency (PLQE)
Photoluminescence quantum efficiency was performed on a home-built measurement setup consisting of an integrating sphere (Labsphere 4P-GPS-053-SL), collection fibre (Andor SR-OPT8019), spectrograph (Andor Kymera-328i) and detector (Andor iDus 420). The setup was calibrated for spectral sensitivity with a NIST-traceable quartz-tungsten-halogen lamp (Newport 63967-200QC-OA).
Excitation was performed with a 520 nm temperature-controlled diode laser (Thorlabs). PLQE values were determined via the integrating sphere method. 125 Absorption Spectroscopy
Linear absorption spectra of colloidal nanoplatelets solutions, placed in a 1 mm path length cuvette (Hellma), were measured using a commercial PerkinElmer Lambda 750 UV-vis-NIR setup equipped with a 10 cm integrating sphere module attachment. A Xe lamp was used as an excitation source, and all measurements were performed under standard ambient conditions. In order to collect the scattered light, the sample cuvette was placed on the front window of the sphere. The spectra were measured simultaneously with the solvent hexane to correct for its absorption.
6
Near-field optical characterization of materials
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The NF-tr and NF-PL measurement was performed on the home-built SNOM instrument [47] . A xenon lamp (EQ-99; Energetiq Technology) was used as a white light source with color glass FGS600 to filter the white light spectrum. After fiber coupling, 1.7 mW of broadband light was focused by the first objective lens (50× NA 0.5) into the SNOM tip. Transmitted light was collected by the second objective lens (100× NA 0.9) and guided through a 200-µm fiber into the spectrometer (Kymera-328i, Andor) with a cooling camera (DU420A-BEX2-DD, Andor).
The a-SNOM tips were made of SiO2 hollow-pyramid tips (Nanosensors), and the fabrication processes could be found in [47] . NF-PL was acquired in the same experimental configuration as NF-tr. 6.5 mW of 532 nm DPSS laser was focused into the tip, and emitted PL was accumulated with typically 0.2 second integration time at each pixel. FF-tr was performed under 0.2 mW of broadband illumination on the same homemade SNOM microscope without the SNOM tips.
The a-SNOM tips were made of SiO2 hollow-pyramid tips (Nanosensors), and the fabrication processes could be found in [47] . NF-PL was acquired in the same experimental configuration as NF-tr. 6.5 mW of 532 nm DPSS laser was focused into the tip, and emitted PL was accumulated with typically 0.2 second integration time at each pixel. FF-tr was performed under 0.2 mW of broadband illumination on the same homemade SNOM microscope without the SNOM tips.
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