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2 protocols using diacetyl phosphate

1

In Vitro BBB Endothelial Cell Assay

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Hydrogenated phosphatidylcholine (95%-hydrogenated phosphatidylcholine and 0.5%hydrogenated lyso phosphatidylcholine) was gifted from American Lecithin Company (Oxford, CT). Cit-HBr was kindly donated by Lund beck A/S (Copenhagen, Denmark). Diacetyl phosphate, NAG, and cholesterol were purchased from Fisher Scientific (Pittsburgh, PA). PEG 600 was purchased from Croda Inc. (Columbus Circle Edison, NJ). All other solvents were purchased as HPLC grade. Rat Primary Brain Microvascular Endothelial Cells (RPBMECs), Animal Endothelial cell medium supplemented with murine Endothelial Growth Factor (mEGF), fetal bovine serum (FBS), L-glutamine, Antibiotic-Antimycotic and gelatin-based coating solutions were purchased from Cell Biologics (Chicago, IL). Hank’s Buffered Salt Solution (HBSS) was purchased from Thermo Scientific (South Logan, UT). CellTox Green Cytotoxicity Assay kit was gifted by Promega Corporation (Madison, WI). D-Luciferin fluorescence dye was purchased from BD Bioscience (Bedford, MA). Trypsin was purchased from Gibco (Grand Island, NY). HEPES buffer was purchased from MP Biomedical (Solon, OH). Polystyrene nonpyrogenic 96-well plates were obtained from BD Bioscience (Bedford,MA). 24-well trans inserts obtained from Corning Life Sciences (Lowell, MA.).
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2

In Vitro BBB Endothelial Cell Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hydrogenated phosphatidylcholine (95%-hydrogenated phosphatidylcholine and 0.5%hydrogenated lyso phosphatidylcholine) was gifted from American Lecithin Company (Oxford, CT). Cit-HBr was kindly donated by Lund beck A/S (Copenhagen, Denmark). Diacetyl phosphate, NAG, and cholesterol were purchased from Fisher Scientific (Pittsburgh, PA). PEG 600 was purchased from Croda Inc. (Columbus Circle Edison, NJ). All other solvents were purchased as HPLC grade. Rat Primary Brain Microvascular Endothelial Cells (RPBMECs), Animal Endothelial cell medium supplemented with murine Endothelial Growth Factor (mEGF), fetal bovine serum (FBS), L-glutamine, Antibiotic-Antimycotic and gelatin-based coating solutions were purchased from Cell Biologics (Chicago, IL). Hank’s Buffered Salt Solution (HBSS) was purchased from Thermo Scientific (South Logan, UT). CellTox Green Cytotoxicity Assay kit was gifted by Promega Corporation (Madison, WI). D-Luciferin fluorescence dye was purchased from BD Bioscience (Bedford, MA). Trypsin was purchased from Gibco (Grand Island, NY). HEPES buffer was purchased from MP Biomedical (Solon, OH). Polystyrene nonpyrogenic 96-well plates were obtained from BD Bioscience (Bedford,MA). 24-well trans inserts obtained from Corning Life Sciences (Lowell, MA.).
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