Cp7502 j w cp chirasil dex cb chiral column

The yield and enantiomeric excess of R-BPA were analyzed using a Shimadzu GC-2014 gas chromatograph (GC). The sample was analyzed by GC equipped with an Agilent CP7502 J&W CP-Chirasil-Dex CB chiral column (Machery-Nagel; 25 m × 0.25 mm × 0.25 mm). Injector, column, and FID temperature were 250, 110, and 250 °C. H₂ pressure: 83 kPa. Split ratio: 1:15. Retention time of BPO, S-BPA, and R-BPA were 3.165 min, 4.940 min, and 5.473 min. Equations (1) and (2) evaluated the yield (X) and enantiomeric excess of R-BPA (ee_p).
$$X(\%)=\frac{P\times Ms}{Q\times Mp}\times 100\%$$
Ms and Mp are the molecular weight of the substrate and the product. P and Q stand for the mass of the product at the end of the reaction and the initial mass of the substrate.
$$e{e}_p=\frac{C_R-C_S}{C_R+C_S}\times 100\%$$
C_R and C_S stand for the concentration of R-BPA and S-BPA.

The conversion and ee% of R-HPBE were analyzed using a Shimadzu GC-2014 gas chromatograph. “Conversion” represents the catalytic efficiency and was defined as the ratio of the amount of substrate converted to product to the initial amount of substrate. In this study, the samples were analyzed using a gas chromatograph equipped with an Agilent CP7502 J&W CP-ChirasilDex CB chiral column (Machery-Nagel; 25 m × 0.25 mm × 0.25 mm). The injector, column, and FID temperatures were 250, 130, and 250 °C, respectively. The split ratio was 1:15; the flow rate was 2 mL/min; and the retention times of OPBE, (R)-HPBE, and (S)-HPBE were 18.5, 25.5, and 26.5 min, respectively.
Equations (1) and (2) define the conversion and ee% of (R)-HPBE, as follows: $$X\left(\%\right)=\frac{M_S\times P}{M_P\times Q}\times 100\%$$
where M_S is the molecular weight of the substrate, M_P is the molecular weight of the product, Q is the mass of the substrate at the initial reaction, and P is the mass of the product at the end of the reaction, and
$$ee\%=\frac{C_R-C_S}{C_R+C_S}\times 100\%$$
where C_R and C_S represent the concentrations of (R)-HPBE and (S)-HPBE, respectively.