96 well filter plate

Manufactured by Pion Inc

The 96-well filter plate is a laboratory equipment used to perform filtration and separation processes in a 96-well format. It is designed to efficiently process multiple samples simultaneously, enabling higher throughput in various applications such as sample preparation, purification, and liquid handling.

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Lab products found in correlation

Biomek fx lab, by Beckman Coulter (2 mentions) μsol evolution software, by Pion Inc (2 mentions) Biomek fx, by Beckman Coulter (2 mentions) Biomek fx laboratory automation workstation, by Beckman Coulter (1 mentions)

5 protocols using 96 well filter plate

Automated Solubility Profiling Workflow

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Solubility assays were carried out on a Biomek FX Laboratory Automation Workstation (Beckman Coulter, Inc., Fullerton, CA) using SOL Evolution software (pION Inc., Woburn, MA). The detailed method was as follows. A 10 μL aliquot of 10 mM compound stock (in DMSO) was added to 190 μL of 1-propanol to make a reference stock plate. From this plate, a 5-μL aliquot was mixed with 70 μL of 1-propanol and 75 μL of citrate phosphate-buffered saline (PBS; isotonic) to make the reference plate, and the UV spectrum (250–500 nm) of the reference plate was read. Then, 6 μL of 10 mM test compound stock was added to 594 μL of buffer in a 96-well storage plate and mixed. The storage plate was sealed and incubated at room temperature for 18 h. The suspension was then filtered through a 96-well filter plate (pION Inc.), and 75 μL of filtrate were mixed with 75 μL of 1-propanol to make the sample plate. The UV spectrum of the sample plate was then read. Calculations were carried out with μSOL Evolution software, based on the area under the curve (AUC) of the UV spectra of the sample and reference plates. All compounds were tested in triplicate.

Hazlitt R.A., Teitz T., Bonga J.D., Fang J., Diao S., Iconaru L., Yang L., Goktug A.N., Currier D.G., Chen T., Rankovic Z., Min J, & Zuo J. (2018). Development of Second-Generation CDK2 Inhibitors for the Prevention of Cisplatin-Induced Hearing Loss. Journal of medicinal chemistry, 61(17), 7700-7709.

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Automated Solubility Assay Protocol

Cited 2 times

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The solubility assay [27 (link),28 (link)] was carried out on a Biomek FX lab automation workstation (Beckman Coulter, Inc.) using μSOL Evolution software (pION, Inc.) as follows: 10 μL of compound stock was added to 190 μL of 1-propanol to make a reference stock plate. Next, 5 μL of this reference stock plate was mixed with 70 μL of 1-propanol and 75 μL of PBS (pH 7.4) to make the reference plate, and the UV spectrum (250–500 nm) of the reference plate was read. Then, 6 μL of 10 mM test compound stock was added to 600 μL of PBS, pH 7.4, in a 96-well storage plate and mixed. The storage plate was sealed and incubated at room temperature for 18 h. The suspension was then filtered through a 96-well filter plate (pION Inc.). Next, 75 μL of filtrate was mixed with 75 μL of 1-propanol to make the sample plate, and the UV spectrum of the sample plate was read. Calculations were done using μSOL Evolution software based on the area under the curve (AUC) of the UV spectrum of the sample plate and the reference plate. All compounds were tested in triplicate.

Ortiz D., Guiguemde W.A., Johnson A., Elya C., Anderson J., Clark J., Connelly M., Yang L., Min J., Sato Y., Guy R.K, & Landfear S.M. (2015). Identification of Selective Inhibitors of the Plasmodium falciparum Hexose Transporter PfHT by Screening Focused Libraries of Anti-Malarial Compounds. PLoS ONE, 10(4), e0123598.

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Solubility Determination using Automated Workstation

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Solubility assays were carried out on a Biomek FX lab automation workstation (Beckman Coulter, Inc., Fullerton, CA) using µSOL Evolution software (pION Inc., Woburn, MA). Compound stock (10 mM in DMSO, 10 µL) was added to 1-propanol (190 µL) to make a reference stock plate. Reference stock solution (5 µL) was mixed with 1-propanol (70 µL) and citrate phosphate buffered saline (75 µL) to make the reference plate and the UV spectrum (250 nm–500 nm) of the sample plate was read. Test compound stock (10 mM in DMSO, 6 µL) was added to buffer (594 µL) in a 96-well storage plate and mixed. The storage plate was sealed and incubated at room temperature for 18 h. The suspension was then filtered through a 96-well filter plate (pION Inc., Woburn, MA). Filtrate (75 µL) was mixed with 1-propanol (75 µL) to make the sample plate, and the UV spectrum (250 nm – 500 nm) of the sample plate was read. Calculation was carried out by µSOL Evolution software based on the AUC_inf (area under curve) of UV spectrum of the sample plate and the reference plate. All compounds were tested in triplicate.

Chen Y., Zhu F., Hammill J., Holbrook G., Yang L., Freeman B., White K.L., Shackleford D.M., O’Loughlin K.G., Charman S.A., Mirsalis J.C, & Guy R.K. (2021). Selecting an anti-malarial clinical candidate from two potent dihydroisoquinolones. Malaria Journal, 20, 107.

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Automated Solubility Screening Protocol

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Solubility assay was carried out on Biomek FX lab automation workstation (Beckman Coulter, Inc., Fullerton, CA) using mSOL Evolution software (pION Inc., Woburn, MA). The detailed method is described as follows: 10 mL of 10 mM compound stock (in DMSO) was added to 190 mL 1-propanol to make a reference stock plate. 5 mL from this reference stock plate was mixed with 70 mL 1-propanol and 75 mL citrate phosphate buffered saline (isotonic) to make the reference plate, and the UV spectrum (250–500 nm) of the reference plate was read. 6 mL of 10 mM test compound stock was added to 594 mL buffer (at pH 3 or 5 or 7.4) in a 96-well storage plate and mixed. The storage plate was sealed and incubated at room temperature for 18 h. The suspension was then filtered through a 96-well filter plate (pION Inc., Woburn, MA). 75 mL filtrate was mixed with 75 mL 1-propanol to make the sample plate, and the UV spectrum of the sample plate was read. Calculations were carried out by mSOL Evolution software based on the AUC (area under curve) of UV spectrum of the sample plate and the reference plate. All compounds were tested in triplicates.

Verlinden B.K., de Beer M., Pachaiyappan B., Besaans E., Andayi W.A., Reader J., Niemand J., van Biljon R., Guy K., Egan T., Woster P.M, & Birkholtz L.M. (2015). Interrogating alkyl and arylalkylpolyamino (bis)urea and (bis)thiourea isosteres as potent antimalarial chemotypes against multiple lifecycle forms of Plasmodium falciparum parasites. Bioorganic & medicinal chemistry, 23(16), 5131-5143.

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Automated Solubility Determination Protocol

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Solubility assays were carried out on a Biomek FX lab automation workstation (Beckman Coulter, Inc., Fullerton, CA) using μSOL Evolution software (pION Inc., Woburn, MA). Compound stock (10 mM in DMSO, 10 μL) was added to 1-propanol (190 μL) to make a reference stock plate. Reference stock solution (5 μL) was mixed with 1-propanol (70 μL) and citrate phosphate buffered saline (pH 7, 75 μL) to make the reference plate which was then measured with UV detection. Test compound stock (10 mM, 6 μL) was added to buffer (594 μL) in a 96-well storage plate and mixed. The storage plate was sealed and incubated at room temperature for 18 hours. The suspension was then filtered through a 96-well filter plate (pION Inc., Woburn, MA). Filtrate (75 μL) was mixed with 1-propanol (75 μL) to make the sample plate, and the UV spectrum (250 nm – 500 nm) of the sample plate was read. Calculation was carried out by μSOL Evolution software based on the AUC (area under curve) of UV spectrum of the sample plate and the reference plate. All compounds were tested in triplicate.

Floyd D.M., Stein P., Wang Z., Liu J., Castro S., Clark J.A., Connelly M., Zhu F., Holbrook G., Matheny A., Sigal M.S., Min J., Dhinakaran R., Krishnan S., Bashyum S., Knapp S, & Guy R.K. (2016). Hit-to-Lead Studies for the Antimalarial Tetrahydroisoquinolone Carboxanilides. Journal of medicinal chemistry, 59(17), 7950-7962.

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