Milli q direct ultrapure water system

DZ and DZ-SIM were synthesized in our laboratory and dissolved in dimethylsulfoxide (DMSO) at a concentration of 20 mM and stored at −20°C. Cisplatin, SIM, Gefitinib, Icotinib, and AZD9291 were purchased from MedKoo Biosciences (Morrisville, NC). Annexin V and propidium iodide (PI) were purchased from Biolegend (San Diego, CA). MitoSOX, MitoTracker, LysoTracker, and JC-1 were purchased from ThermoFisher. For the work of chemical synthesis, all chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO) and/or ThermoFisher Scientific and used as is since the products derived them were purified and analyzed afterwards. Deionized distilled ultrapure water with resistivity of 18.2 MΩ cm used for making solutions was obtained from Milli-Q Direct Ultrapure Water System from Millipore (Billerica, MA, USA). 1H-NMR data were collected on Bruker 400 MHz spectrometers using standard parameters, chemical shifts are reported in ppm (δ) in reference to residual non-deuterated solvent. Electrospray ionization mass spectrometry (ESI-MS) analysis was performed on new compounds at Cedar-Sinai’s Proteomics & Metabolomics Core using an LTQ Orbitrap Elite mass spectrometer.