The following antibodies were used in the study: CDK4 (Abcam, Cambridge, United Kingdom, 1:1,000), CDK6 (Abcam, Cambridge, United Kingdom, 1:50,000), Rb (Abcam, Cambridge, United Kingdom, 1:2000), phospho-Rb S780 (Abcam, Cambridge, United Kingdom, 1:1,000), LC3B (Cell Signaling Technology, Danvers, MA, United States, 1:1,000), P62 (Cell Signaling Technology, Danvers, MA, United States, 1:1,000), LAMP2 (Cell Signaling Technology, Danvers, MA, United States, 1:1,000), β-actin (Beyotime, Shanghai, China, 1:1,000). Abemaciclib and Abemaciclib metabolite M2 were purchased from MedChemExpress (Monmouth Junction, NJ, United States, LY-2835219, LSN2839567). Compounds LPM3770277, LPM3770278, and LPM3770304 were synthesized by Shandong Luye Pharmaceutical Corporation (Yantai, China).
Phospho rb s780
Manufactured by Abcam
Sourced in United Kingdom
Phospho-Rb S780 is a primary antibody that specifically recognizes the phosphorylated form of the retinoblastoma protein (Rb) at serine 780. This antibody can be used to detect and quantify the level of phosphorylation at this site, which is an important regulatory event in cell cycle progression.
Automatically generated - may contain errors
2 protocols using phospho rb s780
1
Antibodies for Cell Cycle and Autophagy Analysis
2
Immunoblotting for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunoblotting was performed using standard protocols. Antibodies were diluted in 5% milk powder inTBS Tween 20 0.1%, and protein detection was carried out with HRP-coupled secondary antibodies and X-ray films. The following primary antibodies were used Axin2 (1:1000, Abcam 109307), SOX2 (1:400, R&D MAB2018), Phospho-Rb (S780) (1:500, Abcam 47763), c-MYC (1:1000, Abcam 32072), FOXG1 (1:50, hybridoma clone 17B12, Pollard lab), WIF1 (1:500, Abcam 186845), NF1 (1:500; Santa Cruz sc-67), PTEN (1:1000; CST 9556), phospho-EGFR Tyr1068 (1:1000; CST 3777), GAPDH (1:40000; ThermoFisher, 6C5), Actin (1:1000; Santa Cruz sc-1616). Quantification of band signal and normalisation to GAPDH signal was performed using ImageJ software and Excel.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!