TG were separated from lipid extracts of Huh-7 cells by TLC in hexane/diethyl ether/acetic acid (70/30/1, v/v). The area corresponding to TG was scraped off the plates and TG was extracted from silica gel. Isolated TG were methylated with 2.5% H2SO4 in methanol. The resulting fatty acid methyl esters were then extracted with hexane and subjected to GC-MS analysis as described previously.38 (link) GC-MS analysis was performed by using an Agilent 7890A-5975C GC-MS network system (Agilent Technologies, Wilmington, Delaware, USA) equipped with a DB-23 capillary column (60 m × 250 μm × 0.15 μm; Agilent Technologies). The oven temperature programme was as follows; the initial temperature was 50°C for 1 min, then raised to 175°C at 25°C/min, to 235°C at 5 °C/min, and held for 5 min. The injector and detector temperatures were both set at 250°C.
Agilent 7890a 5975c gc ms network system
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890A-5975C GC-MS network system is a gas chromatograph-mass spectrometer (GC-MS) instrument designed for analytical applications. It combines a 7890A gas chromatograph and a 5975C mass spectrometer to provide separation, identification, and quantification of complex samples.
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Lab products found in correlation
2 protocols using agilent 7890a 5975c gc ms network system
1
Triglyceride Characterization in Huh-7 Cells
2
Fatty Acid Profiling by GC-MS
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Lipids were extracted by the method of Bligh and Dyer (Bligh and Dyer, 1959 (link)). Isolated phospholipids were methylated with 2.5% H2SO4 in methanol, and then the fatty acid methyl esters were extracted with hexane and subjected to gas chromatography-mass spectrometry (GC-MS) analysis. GC-MS analysis was performed by using an Agilent 7890A-5975C GC-MS network system (Agilent Technologies) equipped with a DB-23 capillary column (60 m × 250 µm × 0.15 µm; Agilent Technologies). The oven temperature program was as follows: an initial temperature of 50°C held for 1 min was raised to 175°C at 25°C/min, and then to 235°C at 5°C/min, and held for 5 min. The injector and detector temperatures were both set at 250°C.
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