Mgcl2 6h2o

Manufactured by Fujifilm

Sourced in Japan

MgCl2·6H2O is a chemical compound that is commonly used in laboratory equipment. It is a crystalline solid that is soluble in water and other polar solvents. The primary function of MgCl2·6H2O is to serve as a source of magnesium and chloride ions in various scientific applications and experiments.

Automatically generated - may contain errors

Lab products found in correlation

Hydrochloric acid (hcl), by Kanto Chemical (2 mentions) Cacl2, by Fujifilm (2 mentions) Sodium chloride (nacl), by Fujifilm (2 mentions) Potassium chloride (kcl), by Fujifilm (2 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions) Dnase, by Roche (1 mentions) Potassium chloride (kcl), by Kanto Chemical (1 mentions) Sodium hydroxide (naoh), by Kanto Chemical (1 mentions) K2hpo4, by Fujifilm (1 mentions) Nh4cl, by Fujifilm (1 mentions) Na2so4, by Kanto Chemical (1 mentions) Nahco3, by Kanto Chemical (1 mentions) Proteinase k, by Takara Bio (1 mentions) Agar powder, by Fujifilm (1 mentions) Yeast extract, by Fujifilm (1 mentions) Chymotrypsin, by Nacalai Tesque (1 mentions) Kh2po4, by Fujifilm (1 mentions) Fecl3 6h2o, by Fujifilm (1 mentions) Dnase 1, by Roche (1 mentions) Gibco penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

MgCl2 (25 citations)

7 protocols using mgcl2 6h2o

DNase Treatment for Porcine Aorta Extraction

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The DNase treatment
has been slightly
modified based on a previous study.^{41 (link)} DNA
was fragmented using 30 mL of DNase saline solution on 1.0 g of the
DME-extracted porcine aorta. The saline solution was prepared with
deionized water containing 0.9% NaCl and 1% penicillin and streptomycin
(Thermo Fisher Scientific, Kanagawa, Japan). An enzyme solution was
prepared by adding 0.2% DNase (Roche Diagnostics, Tokyo, Japan) and
0.05 mol/L MgCl₂·6H₂O (Wako, Osaka, Japan)
to the saline. The prepared DNase solution was handled in a clean
bench while avoiding contact with ambient air. After DME extraction,
the porcine aorta was shaken in the DNase–saline solution at
4 °C for 1–7 days.

Kanda H., Ando D., Hoshino R., Yamamoto T., W.a.h.y.u.d.i.o.n.o., Suzuki S., Shinohara S, & Goto M. (2021). Surfactant-Free Decellularization of Porcine Aortic Tissue by Subcritical Dimethyl Ether. ACS Omega, 6(20), 13417-13425.

+ Open protocol

+ Expand

Recombinant Protein Production in E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols

BP1 was produced by a fermentation process using E.coli, which was previously reported in the literature^{28 ,44} and provided in an unprocessed powder form by Spiber Inc. The amino acid composition of BP1 was alanine (16.4%), tyrosine (12.0%), glutamine (23.7%), glycine (21.9%), proline (15.3%), serine (9.3%) and others (1.4%). The protein sequence structure is shown in Fig. 1.
KH₂PO₄, K₂HPO₄, NaCl, Na₂HPO₄·H₂O, NH₄Cl, MgCl₂·6H₂O, CaCl₂, FeCl₃·6H₂O, Yeast extract, and agar powder were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). Na₂SO₄, KCl, HCl, Na₂SO₄, NaHCO₃, and NaOH were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Plysurf was purchased from DKS Co. Ltd. (Kyoto, Japan). All chemicals were of reagent grade and used without further purification. Pronase E (P5147 Protease Type XIV from Streptomyces griseus) was purchased from Sigma Aldrich, Proteinase K was purchased from TaKaRa Bio Inc (Kusatsu, Japan), and chymotrypsin was purchased from NACALAI TESQUE, INC (Kyoto, Japan).

Tachibana Y., Darbe S., Hayashi S., Kudasheva A., Misawa H., Shibata Y, & Kasuya K.I. (2021). Environmental biodegradability of recombinant structural protein. Scientific Reports, 11, 242.

+ Open protocol

+ Expand

Decellularization of Mouse Uterine Tissue

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adult donor female mice (8–16 weeks old) with normal estrus cycles were sacrificed painlessly under the overdose of isoflurane. Uterine horns were excised and dissected into 5 × 2 mm rectangular fragments with all the layers, including myometrium, stroma, and luminal epithelium, and briefly washed with PBS after the removal of connective and fat tissues. For the pregnancy experiments, DUMs were prepared as 10 × 2 mm rectangular fragments. In the ovariectomized mouse DMT model, uterine samples were harvested from the ovariectomized mice that underwent ovariectomy 2 weeks before sacrifice and cut into 3 × 1 mm rectangular fragments.
For decellularization of mouse uteri, the collected uterine fragments were treated by SDS (Wako), as we described previously (3 (link)). Briefly, the tissue fragments from donor mice were immersed in 1% of SDS with PBS solution at room temperature. After SDS treatment, samples were washed with washing buffer containing 0.9% NaCl (WAKO), 0.05 M MgCl₂/6 H₂O (WAKO), 0.2 mg/ml DNase I (Roche Diagnostics), and 1% Gibco penicillin-streptomycin solution (Thermo Fisher Scientific) for 1 week at 4°C on a shaker set at frequency of 1 Hz with daily buffer exchange.

Hiraoka T., Hirota Y., Saito-Fujita T., Matsuo M., Egashira M., Matsumoto L., Haraguchi H., Dey S.K., Furukawa K.S., Fujii T, & Osuga Y. (2016). STAT3 accelerates uterine epithelial regeneration in a mouse model of decellularized uterine matrix transplantation. JCI Insight, 1(8), e87591.

+ Open protocol

+ Expand

Extraction and Characterization of Japanese Cedar

Check if the same lab product or an alternative is used in the 5 most similar protocols

1-Methylimidazolium hydrogen sulfate ([MIM]HSO₄) as shown in Fig. 5 was purchased from Sigma-Aldrich (St. Louis, MO USA). Japanese cedar (Cryptomeria japonica) was collected in our university forest with permission from our university and handled in accordance with relevant guidelines and regulations. Wood flours from Japanese cedar (Cryptomeria japonica) were extracted with ethanol/benzene (1/2, v/v) for 24 h in a Soxhlet apparatus. The wood flours were oven-dried at 105 °C for 24 h before use. 5-Hydroxymethylfurfrural (5-HMF), LiCl, MgCl₂·6H₂O, KCl, ethanol, acetonitrile, acetaldehyde, benzaldehyde, acetone, diethyl ether, tetrahydrofuran (THF), 1-butanol, methyl isobutyl ketone (MIBK), butyl acetate, benzene, and dichloromethane (DCM) were purchased from Wako Pure Chemicals Industries (Osaka, Japan). In this study, an electronic balance (ATX224, Shimadzu Corporation, Kyoto, Japan; Precision: below 0.1 mg) was used for all mass measurement.Figure 5

Structural formula of 1-methylimidazolium hydrogen sulfate ([MIM]HSO₄).

Ito R, & Miyafuji H. (2021). Production of 5-hydroxymethylfurfural from Japanese cedar (Cryptomeria japonica) in an ionic liquid, 1-methylimidazolium hydrogen sulfate. Scientific Reports, 11, 22790.

+ Open protocol

+ Expand

Apatite Formation on Glass Surfaces in SBF

Check if the same lab product or an alternative is used in the 5 most similar protocols

In order to investigate the reaction of the examined glasses with body fluid, change in the surface structure of the glasses in a SBF was studied by the following method.
Glass pates 25 × 35 × 08 mm³ in size were soaked in 100 ml of a SBF in which the ion concentrations (Na⁺ 142.0, K⁺ 5.0, Ca²⁺ 2.5, Mg²⁺ 1.5, Cl^– 147.8, HCO^3– 4.2, HPO₄^2– 1.0, and SO₄^2– 0.5 mM) were nearly equal to those of human blood plasma at 36.5 °C, and the area of the glass plate contact with SBF was maintained at 13 mm²/ml. The SBF was prepared by dissolving reagent grade NaCl, NaHCO_3, KCl, K₂HPO₄•3H₂O, MgCl₂•6H₂O, CaCl₂, and Na₂SO₄ (FUJIFILM Wako Chemicals, Japan) in ultrapure water, and then buffered at pH = 7.4 with tris(hydroxymethyl)aminomethane (CH₂OH)₃CNH₂ (FUJIFILM Wako Chemicals, Japan) and 1 M HCl (Kanto Chemical Co., Inc. Japan) at 36.5 °C [19 (link)]. After soaking in the SBF for 1 week, the samples were gently removed from the SBF and dried in air without washing to prevent any peeling off of the surface layers from the glass substrates. Apatite formation on their surfaces was examined by thin-film X-ray diffraction (SmartLab, Rigaku Co., Japan).

Yamaguchi S., Takeuchi T., Ito M, & Kokubo T. (2022). CaO-B2O3-SiO2 glass fibers for wound healing. Journal of Materials Science. Materials in Medicine, 33(2), 15.

+ Open protocol

+ Expand

Measuring Alkaline Phosphatase Activity in Caco-2 Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alkaline phosphatase (AP) activity was measured as previously described [15 (link),16 (link)] with some modifications. Caco-2 cells were treated with or without 2 mM IIAEK. Micelles were prepared as previously described [13 (link)]. Following treatment, the cells were washed twice with 0.9% NaCl. Subsequently, 691 μL of cold 50 mM Tris buffer [pH 7.5, Trizma base (Sigma-Aldrich, St. Louis, MO, USA, T1503)] was added. Protein was collected on ice and homogenized with an injection needle (26G × 1/2) (TERUMO, Tokyo, Japan, NN-2613S). AP activity was estimated using 1.25 mg/mL disodium p-nitrophenol phosphate as a substrate in Tris-HCl buffer [pH 10.0, 5 mM MgCl₂.₆H₂O (Wako, 135-00165) and 200 mM Trizma base (Sigma-Aldrich, St. Louis, MO, USA, T1503)]. After 30 min of incubation at 37 °C, the absorbance at 405 nm was measured, and AP activity was calculated as μmol/min using a calibration curve for various concentrations of p-nitrophenol. μmol/min was defined as U. U was converted to U/mg protein in order to normalize AP activity to the protein concentration, which was determined using a commercially available kit (BioRad, protein assay; BioRad).

Takeuchi A., Hisamatsu K., Okumura N., Sugimitsu Y., Yanase E., Ueno Y, & Nagaoka S. (2020). IIAEK Targets Intestinal Alkaline Phosphatase (IAP) to Improve Cholesterol Metabolism with a Specific Activation of IAP and Downregulation of ABCA1. Nutrients, 12(9), 2859.

+ Open protocol

+ Expand

Lateral Flow Test Device Assembly

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse IgG (I5381), anti-mouse IgG (M8642) and anti-mouse IgG-alkaline phosphatase (ALP) conjugate (A3562) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nitrocellulose membranes with laminated backing (HF180MC100) and glass fiber conjugate pads (GFDX203000) were purchased from Merck Millipore (Billerica, MA, USA). Absorbent pads (CF7) and Whatman No. 4 filter paper were purchased from GE Healthcare Life Sciences (Buckinghamshire, UK). The BCIP/NBT (5-bromo-4-chloro-3-indolylphosphate p-toluidine salt, nitro-blue tetrazolium chloride) enzyme substrate solution kit was purchased from Nacalai Tesque (Kyoto, Japan). Acid Red 52, Evans Blue, sucrose, bovine serum albumin (BSA), Tween 20, MgCl2•6H2O, casein, boric acid, Na2HPO4 and NaH2PO4•2H2O were purchased from Wako Pure Chemical Industries (Osaka, Japan). Tris(hydroxymethyl) aminomethane was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
A ColorQube 8570 wax printer (Xerox, Norwalk, CT, USA) was used to pattern the hydrophobic wax barrier onto the filter paper substrate. A QHE325 hot laminator (Meikoshokai Co., Ltd., Tokyo, Japan) was used for the post-print heating of the wax. A DC-200N disc cutter (Carl Jimuki Co., Ltd., Tokyo, Japan) was used to cut the porous substrate materials required for the construction of the LFT device.

Ishii M., Preechakasedkit P., Yamada K., Chailapakul O., Suzuki K, & Citterio D. (2018). Wax-Assisted One-Step Enzyme-Linked Immunosorbent Assay on Lateral Flow Test Devices. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 34(1).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!