Digital slr camera

All first generation cig-a-likes were cut below the battery-cartomizer interface to reveal the intact atomizing unit. The underlying fibers were removed using forceps, exposing the wires, the joints between the wires, air-tube, wick, and sheaths. For second and third generation clearomizer and mod-style ECs, the atomizing units were split where the filament was located, with the exception of the RDAs, which were solid units. The components of interest were dissected from each atomizing unit as described previously [20 (link),36 (link)], and the following were recorded: the lab inventory letter code assigned to each unit, EC style, brand, year purchased, type of activation, flavor, nicotine concentration, presence of fibers, whether the Polyfil was centrifuged after dissection, the amount of fluid recovered upon centrifugation, fluid color, presence of a filament, thick wire, wick, air-tube, sheath, number of sheaths, wire-to-wire joints, integrity of the wire, condition of the joints and wick, and evidence of use before purchase. All dissections were photographed using a Canon SLR digital camera, and individual components were imaged using the Nikon SMZ 745 stereomicroscope. All dissections were done on unused products, except for NJOY NPRO 2N1 (2011), which had been used by us prior to dissection.

The extent of corneal wound closure was examined by corneal fluorescein staining as previously described [19 (link),20 (link)]. 24 and 48 h after corneal abrasion, mice were anesthetized with an intraperitoneal injection of the rodent combination anesthesia. A 1 μL amount of fluorescein (0.1%) was instilled on the injured corneas for 1 min, followed by rinsing with 1 mL of BSS. Images were then recorded by an SLR digital camera (Nikon Inc, Melville, NY, USA) and a stereoscopic zoom microscope (SMZ25; Nikon Instruments, Melville, NY, USA), with a fluorescence excitation at 470 nm. Three animals per group were used. Wound areas were analyzed by ImageJ software (U.S. National Institutes of Health, Bethesda, MD, USA)

Naive female C57BL/6 mice 6 to 8 weeks of age (The Jackson Laboratory, Bar Harbor, ME, USA) were anesthetized with an intraperitoneal injection of the rodent combination anesthesia previously mentioned, combined with topical anesthesia applied on the right eyes [19 (link)]. Whatman filter paper (2.5-mm diameter) soaked in 1 N NaOH solution, was placed on the right cornea for 30 s and then the eye was immediately rinsed with 20 mL of BSS. Mice corneas were monitored daily using a stereoscopic zoom microscope (SMZ25; Nikon Instruments, Melville, NY, USA) for 10 days, and images were recorded by an SLR digital camera (Nikon Inc, Melville, NY, USA). For treatment, each day a wafer was placed on top of the ocular burn cornea of an anesthetized mouse for 10 days corresponding to the treatment group. All mice then received 5 μL of BSS on the ocular burn cornea, including uninjured (control) groups.