All animal studies were approved by the UIC Animal Care and Use Committee. Eight-week-old DBA1 mice were immunized with collagen type II (Chondrex, Redmond, WA) on days 0 and 21 [55 (link)–58 ]. CIA mice were treated i.p. 3 times weekly with 2-DG (7.5mg/kg, Sigma), IACS-010759 (10mg/kg; ChemieTek) [19 (link)] or control starting on day 25 until day 51 [55 (link),56 (link),59 (link),60 (link),57 (link),58 ]. Mice were sacrificed on day 52 post-onset and ankle circumference was measured with Caliper [59 (link)]. At the end of the experiment, ankles were dissected and homogenized for qRT-PCR (Trizol), ELISA (PBS+ protease inhibitor cocktail; Roche Diagnostics), or processed for immunohistochemistry (10% formalin).
Pbs protease inhibitor cocktail
Manufactured by Roche
PBS+ protease inhibitor cocktail is a laboratory reagent used to inhibit the activity of proteases in biological samples. It is a concentrated solution containing a mixture of protease inhibitors that act on a broad spectrum of proteases. The product is designed to help preserve the integrity of proteins during sample preparation and analysis.
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Lab products found in correlation
2 protocols using pbs protease inhibitor cocktail
1
Collagen-Induced Arthritis in Mice
2
Collagen-Induced Arthritis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All animal studies were approved by the UIC Animal Care and Use Committee. Eight-week-old DBA1 mice were immunized with collagen type II (Chondrex, Redmond, WA) on days 0 and 21 [55 (link)–58 ]. CIA mice were treated i.p. 3 times weekly with 2-DG (7.5mg/kg, Sigma), IACS-010759 (10mg/kg; ChemieTek) [19 (link)] or control starting on day 25 until day 51 [55 (link),56 (link),59 (link),60 (link),57 (link),58 ]. Mice were sacrificed on day 52 post-onset and ankle circumference was measured with Caliper [59 (link)]. At the end of the experiment, ankles were dissected and homogenized for qRT-PCR (Trizol), ELISA (PBS+ protease inhibitor cocktail; Roche Diagnostics), or processed for immunohistochemistry (10% formalin).
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