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Anti pdk4 antibody

Manufactured by Abcam
Sourced in United States

The Anti-PDK4 antibody is a tool used for the detection and analysis of the PDK4 protein in various biological samples. PDK4 is an enzyme that plays a key role in the regulation of metabolism. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of PDK4 in cells and tissues.

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2 protocols using anti pdk4 antibody

1

Immunostaining of Dystrophin and PDK4

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Frozen sections were fixed in methanol, blocked with albumin (Sigma, St. Louis, MO), and incubated with primary antibodies for 1 hour at 25°C (anti-dystrophin antibody, 1:400 dilution, Abcam, Cambridge, MA, USA; anti-PDK4 antibody 1:500 dilution). Immunostaining was performed using standard procedures as we described previously [14 (link)].
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2

Western Blot Analysis of Cellular Proteins

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Following treatment or transfection, culture supernatants were removed, cells or tissues were lysed using radio immunoprecipitation assay buffer (RIPA, Beyotime, China), and protein was harvested and quantified by bicinchoninic acid (BCA) analysis (Beyotime, China). Protein extracts were separated in 10% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (Sigma-Aldrich, USA). After incubation with a high affinity anti-c-Myc antibody (1:1000, Abcam), anti-ELK1 antibody (1:1000, Abcam), anti-cdc25c antibody (1:1000, Abcam), anti-hif1 antibody (1:1000, Abcam), anti-c-Jun antibody (1:1000, Abcam), anti-Mdm2 antibody (1:1000, Abcam), anti-JunB antibody (1:1000, Abcam), anti-mct4 antibody (1:1000, Abcam), anti-pdk1 antibody (1:1000, Abcam), anti-pdk4 antibody (1:1000, Abcam), anti-GLUT1 antibody (1:1000, Abcam), anti-GLUT4 antibody (1:1000, Abcam), anti-Ubiquitin antibody (1:1000, Abcam) or anti-β-actin antibody (1:2000, Cell Signaling Technology, USA) in Primary Antibody Dilution (MB9881, Dalian Meilun Biotechnology Co., Ltd), the membranes were incubated with a secondary antibody (1:5000, Cell Signaling Technology, USA). After washes, signals were detected using FDbio-Femto ECL (Fudebio, Hangzhou, China) and a chemiluminescence system (Bio-Rad, USA). All images were analyzed using Image Lab software.
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