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Balb c rag2 γc mice

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BALB/c RAG2−/− γc−/− mice are genetically engineered laboratory mice. They lack mature T cells and B cells, and have impaired natural killer cell function.

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Lab products found in correlation

Green cmfda, by Thermo Fisher Scientific (2 mentions) Rosettesep human monocyte enrichment cocktail, by STEMCELL (2 mentions)

2 protocols using balb c rag2 γc mice

1

In Vivo Lung Metastasis Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Forty-eight hours after transfection, A375M2 cells were labelled with 10 μM CMFDA-Green (Life Technologies), trypsinized and injected into the tail vein of BALB/c RAG2−/− γc−/− mice (male and female between 6–12 weeks old (The Jackson Laboratory); mice were age- and sex-matched between the groups) together with human monocytes (previously shown to enhance lung retention of cancer cells69 (link)), 1 × 106 A375M2 and 1 × 106 monocytes in 0.2 ml PBS. Mice were killed after 1 h (to show that equal numbers arrive at the lung) and 10 h, the lungs were washed, fixed (4% formaldehyde for 16 h) and examined for fluorescently labelled cells under a confocal microscope (see above). Lung retention is represented as fluorescence signal (CMFDA-Green from melanoma cells) per field, and ~20 fields per mouse were analysed. Each experiment had four mice per condition, and experiments were replicated three times. RosetteSep Human Monocyte Enrichment Cocktail (StemCell Technologies no. 15028) was used to purify human monocytes.
Orgaz J.L., Pandya P., Dalmeida R., Karagiannis P., Sanchez-Laorden B., Viros A., Albrengues J., Nestle F.O., Ridley A.J., Gaggioli C., Marais R., Karagiannis S.N, & Sanz-Moreno V. (2014). Diverse matrix metalloproteinase functions regulate cancer amoeboid migration. Nature communications, 5, 4255.
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2

In Vivo Lung Metastasis Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Forty-eight hours after transfection, A375M2 cells were labelled with 10 μM CMFDA-Green (Life Technologies), trypsinized and injected into the tail vein of BALB/c RAG2−/− γc−/− mice (male and female between 6–12 weeks old (The Jackson Laboratory); mice were age- and sex-matched between the groups) together with human monocytes (previously shown to enhance lung retention of cancer cells69 (link)), 1 × 106 A375M2 and 1 × 106 monocytes in 0.2 ml PBS. Mice were killed after 1 h (to show that equal numbers arrive at the lung) and 10 h, the lungs were washed, fixed (4% formaldehyde for 16 h) and examined for fluorescently labelled cells under a confocal microscope (see above). Lung retention is represented as fluorescence signal (CMFDA-Green from melanoma cells) per field, and ~20 fields per mouse were analysed. Each experiment had four mice per condition, and experiments were replicated three times. RosetteSep Human Monocyte Enrichment Cocktail (StemCell Technologies no. 15028) was used to purify human monocytes.
Orgaz J.L., Pandya P., Dalmeida R., Karagiannis P., Sanchez-Laorden B., Viros A., Albrengues J., Nestle F.O., Ridley A.J., Gaggioli C., Marais R., Karagiannis S.N, & Sanz-Moreno V. (2014). Diverse matrix metalloproteinase functions regulate cancer amoeboid migration. Nature communications, 5, 4255.
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