A total of 40 male Wistar albino rats, weighing 95-117 g, were purchased from Vac Sera lab., Helwan City, Cairo Governorate, Egypt. The animals were housed in polypropylene cages and kept under standard laboratory conditions of temperature 22-25 °C and 12 h light/12 h dark cycle. Rats were provided with clean water and diet ad libitum. A balanced diet of commercial pellets, (table 1), (Atmida Company for international commerce and development, Egypt). The animals were kept for 10 days before the beginningof the experiments for acclimatization. Animal rearing and handling and the experimental design and procedures were approved by the Research Ethics Committee of the Faculty of Veterinary Medicine, University of Sadat City, Egypt (VUSC-006-2-20). Chemicals A ranitidine was purchased from the Egyptian international Pharmaceuticals industries company (EIPICO), Golf city, Cairo, Egypt.
Diagnostic kits for assaying serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and diagnostic kits for assaying serum levels of total urea, and creatinine were purchased from Diamond Company, Egypt, while kits for assaying reduced glutathione (GSH),malondialdehyde (MDA), and superoxide dismutase (SOD) and Catalase activity (CAT) content in hepatic tissue were purchased from Bio-Diagnostics Ltd., Egypt. Other chemicals used in this study were of analytic grades.
Diagnostic kits for assaying serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and diagnostic kits for assaying serum levels of total urea, and creatinine were purchased from Diamond Company, Egypt, while kits for assaying reduced glutathione (GSH),malondialdehyde (MDA), and superoxide dismutase (SOD) and Catalase activity (CAT) content in hepatic tissue were purchased from Bio-Diagnostics Ltd., Egypt. Other chemicals used in this study were of analytic grades.