Alexa fluor 594 conjugate goat anti rabbit igg h l

The cells were fixed with 4% paraformaldehyde in PBS (pH 7.4) for 15 minutes at room temperature. Fixed cells were washed twice using ice‐cold PBS, permeabilized with 0.1% Triton X‐100 in PBS for 10 minutes, and subsequently blocked for 2 hours at room temperature in PBS containing 5% FBS. The cells were incubated with a blocking buffer containing BCL2 antibodies (12789‐1‐AP, Proteintech) at 4°C overnight. The Alexa Fluor^® 594 conjugate goat anti‐rabbit IgG (H + L; #ZF‐0516; ZSGB‐BIO) was diluted in blocking buffer and incubated at 37°C for 1 hour. After washing with PBS for three times, the nuclei were stained by 10 µg/mL Hoechst 33342 for 8 minutes. Finally, the images were collected by an EVOS fluorescence microscope.²¹

The cells were fixed with 4% paraformaldehyde in PBS (pH 7.4) at room temperature for 10 min [38 (link)]. Fixed cells were washed three times using ice-cold PBS, permeabilized with 0.1% Triton X-100 in PBS for 10 min, and then subsequently blocked for 1 h at room temperature in PBS containing 10% FBS. The cells were incubated with a blocking buffer containing CTNNB1 antibodies (51067-2-AP, Proteintech) at 4 °C overnight. The Alexa Fluor^® 594 conjugate goat anti-rabbit IgG (H + L; #ZF-0516; ZSGB-BIO) was diluted in a blocking buffer and incubated at room temperature for 1 h. After washing with PBS three times, the nuclei were stained with 10 µg/mL DAPI for 10 min. Finally, the images were collected by an EVOS fluorescence microscope.