Tissue samples from kidney, liver, lung, brain, heart, spleen, gastrointestinal tract, pancreas, ovaries and testes were collected and fixed in 10 % phosphate-buffered formalin (pH 7.0) for 24 h, routinely processed, embedded in paraffin wax, cut into 2–3 μm (μm) sections and stained with hematoxylin and eosin (H&E). The photomicrographs were taken using an Olympus SP 350 digital camera and Stream Basic imaging software (Olympus Corporation, Tokyo, Japan) [14 (link)].
Stream basic imaging software
Manufactured by Olympus
Sourced in Japan
Stream Basic is an imaging software developed by Olympus. It provides basic functionality for image acquisition, processing, and analysis.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using stream basic imaging software
1
Histological Analysis of Organ Tissues
2
Histological Analysis of Rat Eyes
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The eyes of rats from the experimental groups (G-I, G-II, G-III) and the control group were collected immediately after euthanasia, fixed in 10% phosphate-buffered formalin with pH 7.0 (24 h), processed through a tissue-specific protocol and embedded in paraffin wax. The 4 micrometer (µm) sections were cut horizontally through the visual axis and routinely stained with hematoxylin and eosin (H&E). The photomicrographs were taken using an Olympus SP 350 digital camera and Stream Basic imaging software (Olympus Corporation, Tokyo, Japan).
The thickness of the iris dilator muscle and the posterior epithelium was evaluated. The animals used in this study were albino Wistar rats; thus an assessment regarding the pigment granules in the posterior epithelium cells could not be performed.
The thickness of the iris dilator muscle and the posterior epithelium was evaluated. The animals used in this study were albino Wistar rats; thus an assessment regarding the pigment granules in the posterior epithelium cells could not be performed.
3
Cytological and Histopathological Analysis of Pulmonary Growths
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Cytological examination was performed from twelve cases (ten of classical form and two of atypical form) by impression smears method and stained using Dia Quick Panoptic technique (DQP, Reagens Kft, Budapest, Hungary).
For histopathology, collected tissue samples were routinely processed for paraffin embedding by using graded concentrations of alcohol for dehydration and xylene. The paraffin blocks were cut at 2–3 μm thickness and stained with hematoxylin-eosin (H&E). In addition, for pulmonary myxoid growths, alcian blue-periodic acid Shiff stain (AB-PAS) was also performed. The tumors were classified according to World Health Organization for domestic animals’ diagnostic criteria [32 , 59 ]. Pulmonary myxoid growths were described according to [41 ]. For both histology and immunohistochemistry, the sections were independently examined by two pathologists (MT and CT) using a light Olympus BX-41 microscope and the photomicrographs were taken using an Olympus SP 350 digital camera and Stream Basic imaging software (Olympus Corporation, Tokyo, Japan). When there was a divergence of opinion, an agreed diagnosis was reached through simultaneous evaluation in a multi-head microscope (Zeiss Axio Scope A1).
For histopathology, collected tissue samples were routinely processed for paraffin embedding by using graded concentrations of alcohol for dehydration and xylene. The paraffin blocks were cut at 2–3 μm thickness and stained with hematoxylin-eosin (H&E). In addition, for pulmonary myxoid growths, alcian blue-periodic acid Shiff stain (AB-PAS) was also performed. The tumors were classified according to World Health Organization for domestic animals’ diagnostic criteria [32 , 59 ]. Pulmonary myxoid growths were described according to [41 ]. For both histology and immunohistochemistry, the sections were independently examined by two pathologists (MT and CT) using a light Olympus BX-41 microscope and the photomicrographs were taken using an Olympus SP 350 digital camera and Stream Basic imaging software (Olympus Corporation, Tokyo, Japan). When there was a divergence of opinion, an agreed diagnosis was reached through simultaneous evaluation in a multi-head microscope (Zeiss Axio Scope A1).
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