Streptavidin horseradish peroxidase hrp

Manufactured by Vector Laboratories

Sourced in United States

Streptavidin–horseradish peroxidase (HRP) is a conjugate of the protein streptavidin and the enzyme horseradish peroxidase. It is used as a detection reagent in various immunoassay techniques, such as enzyme-linked immunosorbent assay (ELISA).

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Lab products found in correlation

Western lightning plus ecl, by PerkinElmer (1 mentions) Chemidoc xr, by Bio-Rad (1 mentions) Alk detection kit, by Nichirei Biosciences (1 mentions) F4 80, by Santa Cruz Biotechnology (1 mentions) Vector novared peroxidase hrp substrate kit, by Vector Laboratories (1 mentions) Dp72 camera, by Olympus (1 mentions) Cellsens software, by Olympus (1 mentions) 96 well plate, by Merck Group (1 mentions)

Spelling variants of this product

Streptavidin-HRP (55 citations) Streptavidin-horseradish peroxidase (45 citations) Horseradish peroxidase (30 citations) Streptavidin-peroxidase (20 citations) Horseradish peroxidase (HRP)-conjugated streptavidin (6 citations) Horseradish peroxidase (HRP) (4 citations) Horseradish peroxidase (HRP)-linked streptavidin (2 citations) Streptavidin-horseradish-peroxidase (HRP)-complex (2 citations)

5 protocols using streptavidin horseradish peroxidase hrp

Glycoprotein Staining of SMME Membranes

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The glycoproteins on the SMME membranes were stained with lectins according to the immunostaining method previously reported^{26 (link)–28 (link)}. Briefly, the electrophoresed membranes were immersed in acetone for 30 min, followed by heating at 150 °C for 5 min^{29 (link),30 (link)}. The membranes were blocked by immersing in 5% BSA or 5% PGM in phosphate-buffered saline (PBS) containing 0.05% Tween-20 (PBS-T) for 1 h and then incubated with biotin-labeled lectins that are, Sambucus sieboldiana agglutinin (SSA) (J-CHEMICAL, Inc. Tokyo, Japan), Aleuria aurantia lectin (AAL) (J-CHEMICAL), Maackia amrensis lectin II (MAL-II) (Vector Laboratories, Inc. Burlingame, California, USA) and N-terminal domain of BC2L-C lectin derived from Burkholderia cenocepacia (BC2LCN) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) in PBS-T (1/500) for 1 h at room temperature, respectively. Lectin binding was visualised by using the streptavidin–horseradish peroxidase (HRP) (Vector) conjugate and a chemiluminescence reagent (Western Lightning Plus-ECL; PerkinElmer, Boston, MA, USA). Chemiluminescence images were obtained by using ChemiDoc XRS (Bio-Rad, Hercules, CA, USA) and visualized using Image J v1.53e.

Sugiura T., Hashimoto K., Kikuta K., Anazawa U., Nomura T, & Kameyama A. (2023). Expression and localisation of MUC1 modified with sialylated core-2 O-glycans in mucoepidermoid carcinoma. Scientific Reports, 13, 5752.

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Immunohistochemical Evaluation of hNLRR1

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The six NB specimens were surgically collected and embedded in paraffin at Kyushu University Hospital. The appropriate informed consent was obtained under the institutional review board-approved protocol. The formalin-fixed, paraffin-embedded specimens and a purchased tissue array MC803a (U.S. Biomax) were deparaffinised and stained with an anti-hNLRR1 antibody (R&D, 1:250) overnight. The signal was detected by a biotin-conjugated secondary antibody and streptavidin-horseradish peroxidase (HRP) (VECTOR), followed by diaminobenzidine staining (VECTOR). An ALK detection kit (Nichirei) was used according to the manufacturer’s protocol. The study was approved by the Kyushu University of Medical Human Investigation Committee and the internal review board of the Chiba Cancer Center. The study was performed in accordance with the approved guideline.

Satoh S., Takatori A., Ogura A., Kohashi K., Souzaki R., Kinoshita Y., Taguchi T., Hossain M.S., Ohira M., Nakamura Y, & Nakagawara A. (2016). Neuronal leucine-rich repeat 1 negatively regulates anaplastic lymphoma kinase in neuroblastoma. Scientific Reports, 6, 32682.

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Adipocyte Morphometry and Macrophage Quantification

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Liver and perigonadal visceral adipose tissue (VAT) was removed, fixed and processed to paraffin blocks. Tissue sections were stained with haematoxylin and eosin (H & E). Adipocyte area and diameter were measured using H & E-stained VAT sections in 9 to 10 fields at 200

\times

-power fields using cellSens software (Olympus, Tokyo, Japan). Adipocyte numbers were determined in 5 fields at 200

\times

-power field. For Oil Red O staining, frozen liver sections were prepared using O.C.T. compound (Tissue-Tek) prior to staining (UHN Pathology Research Program, Toronto, ON, Canada).
Adipose tissue macrophages in VAT sections were detected by immunostaining for F4/80 (1:200 dilution, Santa Cruz Biotechnology) for 2 hours at room temperature (RT). Primary antibodies were detected using goat anti-rat IgG-biotinylated (1:200 dilution, Santa Cruz Biotechnology) with 30 minutes incubation at RT. Secondary antibodies were detected using streptavidin horseradish peroxidase (HRP) (Vector labs) for 30 minutes at RT followed by colour development Vector NovaRED Peroxidase (HRP) Substrate Kit (Vector labs) as per manufacturer’s instructions. CLS were quantified per 100

\times

-power field in at least 15 fields in a blinded manner using Leica DM1000 microscope (Wetzlar, Germany) with Olympus DP72 camera (Waltham, MA, USA).

Desai H.R., Sivasubramaniyam T., Revelo X.S., Schroer S.A., Luk C.T., Rikkala P.R., Metherel A.H., Dodington D.W., Park Y.J., Kim M.J., Rapps J.A., Besla R., Robbins C.S., Wagner K.U., Bazinet R.P., Winer D.A, & Woo M. (2017). Macrophage JAK2 deficiency protects against high-fat diet-induced inflammation. Scientific Reports, 7, 7653.

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Cytokine Secretion in Irradiated Mice

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Three mice from each group were sacrificed on days 3 and 7 after irradiation to collect the spleen. Enzyme-linked immunosorbent spot (ELISPOT) assays were performed to determine LLC1 tumor specific cytokine secretion; IL-2, IFN-γ and IL-6 from untreated and irradiated mice splenocytes. ELISPOTS were carried out as per the manufacturer’s protocols (R&D Systems) using 96-well plates (Millipore). The plates were coated with 10 μg/ml rat anti-mouse IFN-γ or IL-2 or IL-6 antibodies in PBS at 4°C overnight. The plates were washed three times with PBS and blocked with complete growth media for 2 h. Frozen mice splenocytes (1.5 × 10⁶ cells/well) were thawed and added to each well of the ELISPOT plate and stimulated for 24 h at 37°C, 5% CO₂, in the presence of LLC1 cell lysate (60 μg/well). After 24 h, spots were developed with biotinylated anti-IFN-γ, or IL-2 or IL-6 antibodies (R&D Systems), followed by Streptavidin-horse radish peroxidase (HRP) (Vector Laboratories, Burlingame, CA) and an AEC, 3-amino-9-ethylcarbazole, substrate kit (Vector Laboratories, Burlingame, CA) according to the manufacturer’s instructions. The membrane was read by automated reader (CTL Immunospot, Shaker Heights, OH) for quantitative analyses of the number of IL-2, IL-6 and IFN-γ spots forming cells (SFC) per 1.5 million cells plated.

Kanagavelu S., Gupta S., Wu X., Philip S., Wattenberg M.M., Hodge J.W., Couto M.D., Chung K.D, & Ahmed M.M. (2014). In Vivo Effects of Lattice Radiation Therapy on Local and Distant Lung Cancer: Potential Role of Immunomodulation. Radiation research, 182(2), 149-162.

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Immunohistochemical Analysis of IL-33 in RCC

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Formalin-fixed, paraffin-embedded RCC tissue sections (5 mm) were dewaxed and rehydrated before an antigen retrieval step. Sections were then incubated with anti-human primary antibodies for IL-33 (Enzo Life Sciences) or matching IgG isotypes overnight. Slides were then stained with species-specific biotinylated secondary antibodies (R&D Systems), streptavidin horseradish peroxidase (HRP), and detected with substrate AEC (3-amino-9-ethylcarbazole; Vector Laboratories). Subsequently, slides were counterstained with hematoxylin. IL-33 expression was evaluated according to the intensity and extent of staining. The proportion of stained cells per specimen was semi-quantitatively evaluated and scored as follows: 0 for staining ≤1%; 1 for 2-25%; 2 for 26-50%; 3 for 51-75%; and 4 for >75% of the examined cells. The staining intensity was stratified as follows: 0, negative staining; 1, weak staining; 2, moderate staining; and 3, strong staining. The results were evaluated using the following formula: total score = proportion score × intensity score. A total score of 0-12 was graded as negative (-; score: 0-4) or positive (+; score: 5-12).

Wu C.W., Wu Y.G., Cheng C., Hong Z.D., Shi Z.M., Lin S.Q., Li J., He X.Y, & Zhu A.Y. (2018). Interleukin-33 Predicts Poor Prognosis and Promotes Renal Cell Carcinoma Cell Growth Through its Receptor ST2 and the JNK Signaling Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(1).

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