CircAtxn7loxp/loxp mice were generated using CRISPR-Cas9-mediated genome editing by Cyagen Biosciences Inc. (China). Immunocompromised NOD.SCID mice were obtained from GemPharmatech (China) and used to establish patient-derived xenograft models. OT-I (C57BL/6-Tg (TcraTcrb)1100Mjb/J), Cd8a-Cre (C57BL/6-Tg (Cd8a-cre)1Itan/J) were provided by the Jackson Laboratory. To generate mice with circAtxn7 conditional deletion in CD8+ T cells, CircAtxn7loxp/loxp mice were crossed with Cd8a-Cre mice. Conditional knockout of circAtxn7 was confirmed by qRT-PCR using T cells puried from the spleen. We genotyped these experiment cohort strains by PCR amplification methods. PCR primers used in genotyping are listed in Supplementary Table 2 . Animals were bred under specific pathogen-free conditions at the Experimental Animal Center of Sun Yat-sen University. Female mice were used for all animal work under the protocols approved by the Institutional Animal Care and Use Committee (IACUC), Sun Yat-sen University (approval number: SYSU-IACUC-2020-000438, SYSU-IACUC-2021-000285 and SYSU-IACUC-2021-000642).
Nod scid mice
Manufactured by GemPharmatech
Sourced in China
NOD/SCID mice are a strain of immunodeficient mice widely used in biomedical research. They lack functional T and B cells, making them a valuable model for studying human disease and evaluating experimental therapies.
Automatically generated - may contain errors
Lab products found in correlation
M csf, by Thermo Fisher Scientific (1 mentions)
Ab182981, by Abcam (1 mentions)
C57bl 6, by GemPharmatech (1 mentions)
Balb c nude mice, by GemPharmatech (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
C57bl 6j mice, by GemPharmatech (1 mentions)
C57bl 6 mice, by GemPharmatech (1 mentions)
17 protocols using nod scid mice
1
Generation of circAtxn7 Conditional Knockout Mice
2
Obtaining Human Blood Samples for Animal Studies
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Human blood buffy coats from healthy donors were obtained from Shenzhen Blood Center (Shenzhen, China), and tested negative for blood-born pathogens using the standard protocols of the blood center. All of the protocols involving human blood samples were approved by the Institutional Review Board of the Shenzhen Blood Center. 5-6 weeks old female NOD/SCID mice were purchased from Gem Pharmatech LLC (Jiangsu, China), 5-6 weeks old female BALB/c mice were purchased from Guangdong Province Laboratory Animal Center (Guangzhou, China) and maintained in the institutional animal care facility. All animal protocols were approved by Institutional Animal Care and Usage Committee of Shenzhen People's Hospital.
3
In Vivo Monocyte Migration Assay
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The in vivo monocyte migration assay was performed according to a previous study, with some modifications. Briefly, 27 male NOD/SCID mice (Gempharmatech) were separated into 3 equal groups. All mice were subcutaneously injected with 0.5 mg/kg human macrophage colony–stimulating factor (M-CSF) (PeproTech) to maintain the survival of monocytes. After 12 hours, MSCs pretreated with METTL16 siRNA or siNC were intraperitoneally injected at 5 × 105 cells per mouse. Equal amounts of PBS were also injected as a negative control. After approximately 30 minutes, monocytes were prepared and stained with CFSE, and the mice were intravenously injected with 2 × 107 cells per mouse. Sixteen hours after adoptive transfer, the mice were sacrificed, and the peritoneum was washed to collect the peritoneal lavage fluid for further flow cytometric analysis.
4
Evaluating AML-MSC and Adriamycin in Mice
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Four‐week‐old female NOD‐SCID mice were obtained from GemPharmatech Co., Ltd and housed in a specific pathogen‐free environment with a 12:12 h light : dark cycle and consistent temperature and humidity. After 1 week of adaptive feeding, all mice were randomly divided into four groups (each with five mice): HL60, HL60+adriamycin, HL60+AML‐MSC, and HL60+AML‐MSC+adriamycin. Mice in HL60 and HL60+adriamycin groups were then inoculated s.c. into groins with 5 × 106 HL60 cells and the other two groups were inoculated s.c. with 5 × 106 HL60 cells mixed with 1 × 106 AML‐MSC cells. Tumor sizes were measured using a vernier caliper when tumors could be visualized. Tumor growth was evaluated by measurement of tumor volumes calculated as V = (length × width2)/2. When the mean tumor volume reached 100 mm3, mice in the HL60+adriamycin and HL60+AML‐MSC+adriamycin groups were i.p. injected with adriamycin at a dose of 1 mg/kg every day; the control groups were treated with saline. Three weeks after injection, mice were killed and tumors were dissected out and measured. All animal experiments were approved by the Ethics Committee of the First Hospital of Lanzhou University.
5
Xenograft Model of Breast Cancer
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NOD/SCID mice (female, 4 weeks old, GemPharmatech, China) were divided into four groups randomly as MDA‐MB‐231‐sh‐NC, MDA‐MB‐231‐sh‐NR2F1, MCF‐7‐lenti‐Vec and MCF‐7‐lenti‐NR2F1 (n = 6/group). 5 × 106 transfected cells were injected into the fat pad under the breast of each mouse. Then, mice were sacrificed and dissected at 4 weeks after injection and tumour masses were weighed and then fixed in formalin for immunohistochemical (IHC) staining. CD31 (Abcam, ab182981, 1:2000, UK) was applied for the staining of endothelial cells to evaluate the micro vessel density (MVD).
6
Xenograft of PANC-1 Cells in NOD-SCID Mice
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Four- to six-week-old NOD-SCID mice (strain no. T001492) (weighing 20–25 g) were obtained from Jiangsu GemPharmatech Co., Ltd (Nanjing, China) for in vivo xenograft experiments. Cultured PANC-1 sgNC or sgHMGA2 cells (3 × 106) were subcutaneously transplanted into the right flank of all mice via randomization to experimental groups. The tumor volume was measured every 2 days, starting 5 days post-transplantation, and calculated using the following formula: tumor volume = (long × wide2) × 1/2. At the end of the experiment, the tumors were imaged and weighed after the mice were euthanized. A total of seven mice were used in each group, and no blinding methods were used. All animal procedures were performed according to protocols approved by the Animal Care Committee of Ningbo University.
7
Xenograft Model of AIMP1 in OCI-MY5 Cells
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AIMP1‐WT and AIMP1‐OE OCI‐MY5 cells (2 × 106) were injected subcutaneously into the abdominal left and right flank of 6‐ to 8‐week‐old NOD/SCID mice (n = 6) (GemPharmatech LLC., Nanjing, Jiangsu, China), respectively. Tumor diameter was measured with calipers 2‐3 times weekly. After 28 days, the mice were euthanized by spinal dislocation when the tumor diameter reached 15 mm. The tumors were isolated, weighed, and imaged. The tumor volumes were calculated using the formula: 0.52 × larger diameter × (smaller diameter)2.
8
Metastasis Study in NOD/SCID Mice
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For animal experiments, we followed the NIH Guide for the Care and Use of Laboratory Animals. In our research, 7‐ to 8‐week‐old female NOD/SCID mice (GemPharmatech LLC.) were used in all animal models. For mouse tail vein metastasis assays, 1 × 106 cells transfected with metastasis‐related gene library were injected into NOD/SCID mice. The fluorescence method was utilized to image tumor in mice to observe the status of tumor metastasis. After 6 weeks, the mice were sacrificed.
9
Murine Model Maintenance Protocols
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Six‐week‐old C57BL/6 and NOD‐SCID mice were purchased from Gempharmatech Company (Shanghai, China). All mice were specific pathogen‐free and housed under high‐barrier conditions, according to the guidelines of the Jackson Laboratory, in the animal facility of the Animal Experimentation Center, Nanjing Agricultural University.
10
Dual-Targeted Therapy for Gastric Cancer
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All experiments were approved by the Ethics Committee of Shanghai Jiao Tong University School of Medicine, and all animal studies were performed following AAALAC guidance. Balb/c nude mice and NOD scid mice were purchased from GemPharmatech Co., Ltd. and maintained under 14-h light/10-h dark cycles (dark 20:00-6:00). The use of the SNU-668 and NUGC-4 cell line-derived xenografts was approved by the Institutional Animal Care and Use Committee (IACUC) of Shanghai WuXi AppTec. Experiments involving DGC cancer PDX models 051009 and 0501013 were approved by IACUC of Nanjing Personal Oncology Biotechnology. Mice developed tumors reaching a volume of 100-200 mm3. Six mice in each group received oral administration once per day of one of the following drug regimens: (1) control vehicle, 25 mg/kg (0.5% Natrosol 250 HX in distilled water); (2) IN10018, 25 mg/kg; (3) crizotinib or entrectinib, 25 mg/kg; or (4) IN10018 plus (S)-crizotinib, 25 mg/kg. Tumor volumes were measured by caliper and calculated. At the appropriate end point, mice were humanely killed, and NUGC-4 tumors were harvested for Western blot analysis.
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