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Nonspecific control shrna

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Nonspecific control shRNA is a laboratory tool used in RNA interference (RNAi) experiments. It is designed to serve as a negative control to account for potential off-target effects of shRNA-based gene knockdown. The core function of this product is to provide a reference point for comparing the effects of specific shRNA-mediated gene silencing.

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Lab products found in correlation

Transfection reagent, by Promega (3 mentions)

Spelling variants of this product

Control shRNA (39 citations)

3 protocols using nonspecific control shrna

1

Silencing LC3B in ARPE-19 Cells

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The LC3B shRNA and the nonspecific control shRNA (Sigma-Aldrich) were transfected into ARPE-19 cells using transfection reagents (Promega, Madison, WI, USA) according to the manufacturer’s protocol. The sequences of human LC3B shRNA were as follow: 5’-CCG GGT TCG GGA TGA AAT TGT CAG TCT CGA GAC TGA CAA TTT CAT CCC GAA CTT TTT TG-3’.The expression of LC3B and β-actin in stable cells was measured.
Hwang N, & Chung S.W. (2020). Sulfasalazine attenuates tamoxifen-induced toxicity in human retinal pigment epithelial cells. BMB Reports, 53(5), 284-289.
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2

Silencing PTX3 in ARPE-19 Cells

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Human PTX3 shRNA and nonspecific control shRNA (Sigma-Aldrich, St. Louis, MO, USA) were transfected into ARPE-19 cells using transfection reagents (#E2691) (Promega, Madison, WI, USA) according to the protocol of the manufacturer. Briefly, for each transfection, shRNA (1 μg) was added to ARPE-19 cells for 24 h, and stable clones expressing shRNA were further selected by puromycin (1.0 μg/mL). Cell culture medium containing puromycin was renewed every 48 h, until resistant colonies could be identified. The expression of PTX3 and the loading control (β-actin) in stable cells was tested.
Hwang N., Kwon M.Y., Woo J.M, & Chung S.W. (2019). Oxidative Stress-Induced Pentraxin 3 Expression Human Retinal Pigment Epithelial Cells Is Involved in the Pathogenesis of Age-Related Macular Degeneration. International Journal of Molecular Sciences, 20(23), 6028.
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3

Silencing mitoNEET in RAW264.7 cells

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The mitoNEET shRNA and the nonspecific control shRNA (Sigma-Aldrich, St Louis, MO) were transfected into RAW264.7 cells using transfection reagents (Promega, Madison, WI, USA) according to the manufacturer’s protocol. The sequences of mouse mitoNEET shRNA were as follows: 5′-CCG GCG TAG GAC CTC TGA TCA TCA ACT CGA GTT GAT GAT CAG AGG TCC TAC GTT TTT TG-3′. The expression of mitoNEET and β-actin in stable cells was measured.
Lee S., Seok B.G., Lee S.J, & Chung S.W. (2022). Inhibition of mitoNEET attenuates LPS-induced inflammation and oxidative stress. Cell Death & Disease, 13(2), 127.
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