Hplc grade phosphoric acid

Manufactured by Avantor

Sourced in France

HPLC grade phosphoric acid is a high-purity chemical compound used in high-performance liquid chromatography (HPLC) applications. It is a colorless, odorless liquid that meets the stringent requirements for HPLC-grade reagents, ensuring consistent performance and reliable results in analytical chemistry procedures.

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2 protocols using hplc grade phosphoric acid

HPLC Analysis of Phenolic Compounds

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HPLC grade methanol, acetonitrile, tetrahydrofuran and isopropanol were purchased from VWR International (Mississauga, ON, Canada). HPLC grade phosphoric acid and acetic acid were purchased from VWR International. The primary grade reference standards chlorogenic acid (purity: 93.9%), rutin (purity: 89.3%), quercetin (purity: 93.4%) and isoquercetin (purity: 93.2%) were purchased from Chromadex (Irvine, CA, USA). Water was deionized using a Barnstead water purification system (Fisher Scientific, Ottawa, ON, Canada). Stock solutions of the reference standards were prepared at 1000 µg/mL. Each day mixed calibration solutions ranging from 0.5 to 200 µg/mL were prepared.

Mudge E., Applequist W.L., Finley J., Lister P., Townesmith A.K., Walker K.M, & Brown P.N. (2016). Variation of Select Flavonols and Chlorogenic Acid Content of Elderberry Collected Throughout the Eastern United States. Journal of food composition and analysis : an official publication of the United Nations University, International Network of Food Data Systems, 47, 52-59.

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Solvents for Analytical Procedures

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Technical grade acetone was purchased from VWR (Haasrode, Belgium). Analytical grade acetone, analytical grade methanol, HPLC gradient grade acetonitrile, HPLC gradient grade methanol, HPLC grade phosphoric acid, HPLC grade formic acid and LC-MS grade formic acid were purchased from VWR International (Fontenay-Sous-Bois, Paris, France). HPLC grade n-otanol and LC-MS grade acetonitrile were purchased from Merck (KGaA, Darmstadt, Germany). Aa grade ethanol (≥99.5%) was purchased from Altia OyJ (Helsinki, Finland). Ultra-pure type I water was purified with Merck Millipore Synergy UV system.

Virtanen V, & Karonen M. (2020). Partition Coefficients (logP) of Hydrolysable Tannins. Molecules, 25(16), 3691.

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