Owing to the limited amount of minor hydrocarbons in the total L race hydrocarbon pool, ozonolysis experiments were conducted only on those hydrocarbons that could be purified to obtain at least 10 mg: lycopadiene, lycopatriene and lycopapentaene. These hydrocarbons were separately dissolved in dichloromethane and submitted to ozone cleavage for 5 min at −78 °C. Each product from reductive cleavage of the resulting ozonide was directly subjected to GC–MS (electron ionization) analyses without purification. GC–MS analysis was carried out using a GCMS-QP2010 Ultra spectrometer (Shimadzu, Kyoto, Japan) equipped with a capillary column (InertCap 1MS, GL Science; 60 m × 0.25 mm, film thickness: 0.25 μm). The column temperature was programmed as follows: 50 °C for 1 min, raised at 10 °C min−1 from 50 to 220 °C, then at 2 °C min−1 from 220 to 260 °C and held for 22 min at the final temperature. Helium was used as a carrier gas at a flow rate of 41.2 cm s−1. Temperatures of injection port, interface and ion source were 260, 250 and 200 °C, respectively.
Gcms qp2010 ultra spectrometer
Manufactured by Shimadzu
Sourced in Japan
The GCMS-QP2010 Ultra is a gas chromatograph-mass spectrometer (GC-MS) system manufactured by Shimadzu. It is a high-performance analytical instrument designed for the separation, identification, and quantification of chemical compounds in complex mixtures. The system combines gas chromatography (GC) for sample separation and mass spectrometry (MS) for compound identification and quantification. The GCMS-QP2010 Ultra offers advanced features and capabilities for a wide range of applications in various industries, including environmental analysis, food and beverage testing, and forensic investigation.
Automatically generated - may contain errors
Lab products found in correlation
Tensor 27 spectrometer, by Bruker (2 mentions)
Inertcap 1ms, by GL Sciences (1 mentions)
Tensor 27 infrared spectrometer, by Bruker (1 mentions)
Bpx 5, by Shimadzu (1 mentions)
Quick start bradford 1x dye reagent, by Bio-Rad (1 mentions)
Avance 3 400 mhz spectrometer, by Bruker (1 mentions)
Ultraflex 3 mass spectrometer, by Bruker (1 mentions)
Spectra system p1000, by Thermo Fisher Scientific (1 mentions)
7 protocols using gcms qp2010 ultra spectrometer
1
Ozonolysis Analysis of Rare Hydrocarbons
2
Essential Oil Extraction from Fruit Peels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the fruit peel was smashed, 15 g of peel powder was hydrated with 300 ml of distilled water in a standard extractor for extracting essential oil for 3 h. Then, the essential oil was dried over anhydrous sodium sulphate until all the water was dried and stored in a dark glass bottle at 4 °C. Gas chromatography–mass spectrometry (GC–MS) analyses were carried out using a Shimadzu GCMS-QP2010 Ultra spectrometer.
3
Comprehensive NMR Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1H (400 MHz)
and 13C (100 MHz) NMR spectra were recorded on a Bruker
Avance III instrument using DMSO-d6, CDCl3, and MeOD-d4 as solvents. 1H and 13C chemical shifts are reported in ppm relative
to tetramethylsilane (0.0). The following abbreviations designate
chemical shift multiplicities: s = singlet, bs = broad singlet, d
= doublet, dd = double doublet, t = triplet, dt = doublet of triplet,
m = multiplet. All 13C NMR spectra are proton-decoupled.
Melting points were determined in open capillary tubes and are uncorrected.
Mass spectra were recorded on a Shimadzu GC–MS-QP-2010 Ultra
spectrometer by direct injection. Elemental analyses were carried
out on a Euro Vector EURO EA 3000 model.
and 13C (100 MHz) NMR spectra were recorded on a Bruker
Avance III instrument using DMSO-d6, CDCl3, and MeOD-d4 as solvents. 1H and 13C chemical shifts are reported in ppm relative
to tetramethylsilane (0.0). The following abbreviations designate
chemical shift multiplicities: s = singlet, bs = broad singlet, d
= doublet, dd = double doublet, t = triplet, dt = doublet of triplet,
m = multiplet. All 13C NMR spectra are proton-decoupled.
Melting points were determined in open capillary tubes and are uncorrected.
Mass spectra were recorded on a Shimadzu GC–MS-QP-2010 Ultra
spectrometer by direct injection. Elemental analyses were carried
out on a Euro Vector EURO EA 3000 model.
4
Purification and Characterization of Aldehydes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aldehydes were purchased from the reagent merchant. The liquid aldehydes were distilled under vacuum before use, while the solid aldehydes were recrystallized in EtOH-H2O under N2 before use. Ethanol was analytical pure (AR) and directly used without any special treatment. All reactions were carried out in N2 and monitored by TLC. Melting points were measured by WRS-2A digital instrument. IR spectra were measured on Bruker Tensor 27 Infrared spectrometer. 1H and 13C NMR spectra were recorded on a Bruker Avance 600/400 instrument (600 or 400 MHz for 1H and 150 MHz for 13C NMR spectroscopy) using CDCl3 as the solvent and Me4Si as the internal standard. Chemical shifts for 1H and 13C NMR were referred to internal Me4Si (0 ppm) and J-values were shown in Hz. Mass spectra were measured on a Shimadzu GCMS-QP2010 Ultra spectrometer (EI).
5
GC-MS Analysis of Derivatized Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This measurement reference to the previous our reported methods [46 (link)]. GC-MS analysis was performed using a GC-MS QP2010 Ultra spectrometer (Shimadzu) with a fused silica capillary column (BPX-5; 30 m × 0.25 mm inner diameter, film thickness: 0.25 μm; Shimadzu) and a front inlet temperature of 25 °C, and a helium gas flow rate through the column of 39.0 cm/s. The column temperature was held at 60 °C for 2 min, then raised by 15 °C/min to 330 °C and maintained for 3 min. The interface and ion source temperatures were 280 and 200 °C, respectively. To perform a semi-quantitative assessment, the peak height of each quantified ion was calculated and normalized using citrate-d4 and 2-isopropylmalate peak height and protein concentration. Protein concentrations were determined with Bio-Rad Quick Start Bradford 1x Dye Reagent according to the manufacturer’s instructions. The retention times and SRM conditions for the derivatized metabolites are summarized in Table S3 .
6
Comprehensive Analytical Characterization
7
Characterization of Polymeric Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents and reagents were purchased from Sigma or Alfa Aesar and were of analytical or HPLC grade. Gel permeation chromatography was carried out using a Thermo Scientific Spectrasystem P1000 isocratic pump equipped with a ultraviolet and refractive index detector and mixed D and mixed E columns. The molecular weights of the polymers were determined by the universal calibration method using polystyrene standards. Tetrahydrofuran (THF) at a flow rate of 1 ml min−1 was used as the mobile phase. NMR studies were carried out on a Bruker Advance DPX 300 NMR Spectrometer (300 MHz). Ultraviolet/visible spectroscopy was performed using a Perkin-Elmer spectrophotometer. FT-IR studies were carried out using a Bruker, Tensor 27 spectrometer. An Oriel 500 W Hg-Xe lamp was used for the photodegradation studies. A Nd:YAG nanosecond laser (7 ns pulse duration) was used for the cell photolysis experiments. Optical microscopy was performed on a Leica DMRM microscope equipped with a digital camera connected to a PC. GC–MS was carried out on a Shimadzu GC–MS-QP2010 Ultra spectrometer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!