Epidermal sheets were prepared as before4 (link) with slight modification. Briefly, ears were split into dorsal and ventral halves with forceps. Thioglycolic acid-containing Hair Removing Body Cream Epilat (Kracie) was used to remove hair in some experiments. Ear halves were incubated for 15 minutes at 37°C on 3.8% ammonium thiocyanate (Wako Pure Chemical Industries) in phosphate buffer (pH 7.0). Epidermal sheets were manually detached from the dermis under a dissecting microscope (Olympus).
Ammonium thiocyanate
Manufactured by Fujifilm
Ammonium thiocyanate is a chemical compound used as a laboratory reagent. It is a colorless, crystalline solid that is soluble in water. Ammonium thiocyanate is commonly used as a component in various analytical and testing procedures in laboratory settings.
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Lab products found in correlation
3 protocols using ammonium thiocyanate
1
Epidermal Sheet Isolation from Mouse Ears
2
Epidermal Isolation from Mouse Ears
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Epidermal sheets were prepared as previously described (Nagao et al., 2009 (link)). Briefly, mouse ears were divided into dorsal and ventral halves with forceps and incubated for 15 min at 37 °C on 3.8% ammonium thiocyanate (Wako Pure Chemical Industries) in phosphate buffer (pH 7.0). The epidermal sheets were manually detached from the dermis under a dissecting microscope (Olympus). Tissue samples for cryosections were embedded in O.C.T. compound (Tissue Tek) and frozen at − 80 °C.
3
Epidermal Sheet Isolation from Mouse Ears
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Epidermal sheets were prepared as before4 (link) with slight modification. Briefly, ears were split into dorsal and ventral halves with forceps. Thioglycolic acid-containing Hair Removing Body Cream Epilat (Kracie) was used to remove hair in some experiments. Ear halves were incubated for 15 minutes at 37°C on 3.8% ammonium thiocyanate (Wako Pure Chemical Industries) in phosphate buffer (pH 7.0). Epidermal sheets were manually detached from the dermis under a dissecting microscope (Olympus).
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