Fluidics station 450 system

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Fluidics Station 450 system is a laboratory instrument designed for automated sample preparation and processing. It offers precise fluid handling capabilities to facilitate various applications in life science research and diagnostics. The system is capable of performing tasks such as hybridization, washing, and staining of samples with a high degree of accuracy and reproducibility.

Automatically generated - may contain errors

Lab products found in correlation

Genechip scanner 3000, by Thermo Fisher Scientific (2 mentions) Genechip 3 ivt express kit, by Thermo Fisher Scientific (2 mentions) Rneasy micro kit, by Qiagen (1 mentions) Nanodrop 2000 spectrometer, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions) Rnase free dnase set, by Qiagen (1 mentions) Hybridization oven 640, by Thermo Fisher Scientific (1 mentions) Genepix 4000b scanner, by Molecular Devices (1 mentions) Genechip mouse genome 430 2.0 array, by Thermo Fisher Scientific (1 mentions) Genechip scanner 3000 7g, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Fluidics Station 450 (353 citations) Fluidics Station (39 citations)

4 protocols using fluidics station 450 system

PBMC RNA Extraction and Microarray Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

After PMBC isolation, 1 × 10⁶ PBMCs were immediately lysed in 1 ml of TRIzol (Life Technologies, Carlsbad, CA, USA) and stored at −80°. The samples were thawed and RNA extracted following the manufacturer’s instructions. The quality of the RNA was assessed using a Nanodrop 2000 Spectrometer (Thermo Scientific, MA, USA) as well as by visualization of the integrity of the 28S and 18S bands on an Agilent Bioanalyzer 2100 instrument (Agilent Technologies, Santa Clara, CA, USA).
Qualified total RNA was further purified using an RNeasy micro kit (QIAGEN, GmBH, Germany). Contaminating genomic DNA was removed using an RNase-Free DNase Set (QIAGEN, GmBH, Germany). The purified RNA was stored at −80° C.
To obtain biotin-labeled cRNA, total RNA was amplified, labeled, and purified using the GeneChip 3’IVT Express Kit (Affymetrix, Santa Clara, CA, USA) following the manufacturer’s instructions. After hybridization on Human PrimeView Arrays for 16 h at 45°C and rotation at 60 rpm in a Hybridization Oven 640 (Affymetrix), slides were washed and stained with a Fluidics Station 450 system (Affymetrix). Scanning was performed on a seventh-generation GeneChip Scanner 3000 (Affymetrix). Affymetrix GCOS software was used to perform image analysis and generate raw intensity data.

Hou J., Wang S., Jia M., Li D., Liu Y., Li Z., Zhu H., Xu H., Sun M., Lu L., Zhou Z., Peng H., Zhang Q., Fu S., Liang G., Yao L., Yu X., Carpp L.N., Huang Y., McElrath J., Self S, & Shao Y. (2017). A systems vaccinology approach reveals temporal transcriptomic changes of immune responses to the yellow fever 17D vaccine. Journal of immunology (Baltimore, Md. : 1950), 199(4), 1476-1489.

+ Open protocol

+ Expand

Microarray Analysis of Rat CMS Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Affymetrix GeneChip Rat 230 2.0 (Affymetrix; Thermo Fisher Scientific, Inc.) was used for microarray analysis. Three rats were randomly selected from each CMS-treated group (0, 25, and 50 mg/kg). Microarray analysis was conducted according to the GeneChip^® 3′ expression array user guide (Affymetrix; Thermo Fisher Scientific, Inc.). cDNA synthesis, cRNA synthesis, biotin labeling, cRNA purification and fragmentation were performed according to the manufacturer's protocols (Affymetrix; Thermo Fisher Scientific, Inc.). The microarrays were hybridized at 45°C at 60 rpm for 16 h. The microarrays were stained and washed using a Fluidics Station 450 system (Affymetrix; Thermo Fisher Scientific, Inc.). A GeneChip^® Scanner 3000 (Affymetrix; Thermo Fisher Scientific, Inc.) was used to scan the microarrays. The reproducibility of the microarray experiments was confirmed using well-defined quality control criteria according to the manufacturer's protocol. The micro-array data was deposited into the Gene Expression Omnibus database (https://www.ncbi.nlm.nih.gov/geo/; no. GSE121792).

Lee E.H., Kim S., Choi M.S., Yang H., Park S.M., Oh H.A., Moon K.S., Han J.S., Kim Y.B., Yoon S, & Oh J.H. (2019). Gene networking in colistin-induced nephrotoxicity reveals an adverse outcome pathway triggered by proteotoxic stress. International Journal of Molecular Medicine, 43(3), 1343-1355.

+ Open protocol

+ Expand

Microarray Analysis of Dexamethasone-Treated BMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The total RNA from subconfluent nontreated BMSCs and BMSCs treated with DEX for 6 h at P5 was isolated and then treated with DNase1 for microarray analysis. Total RNA (1 μg) was amplified and labeled with Cy3 or Cy5 using the Ambion Amino Allyl MessageAmp II aRNA Amplification Kit (Life Technologies, Cat. Number 1753) according to the manufacturer's instructions. An Agilent Whole Human Genome Microarray 4 × 44 K (G4110F) was hybridized with 825 ng of amplified RNA at 65°C for 16 h and washed using a Fluidics Station 450 system (Affymetrix, Inc., Santa Clara, CA, USA). The microarray slides were scanned using a GenePix 4000B scanner from Axon Instruments (Union City, CA, USA), and each microarray image was first analyzed using GenePix Pro 6.1 image analysis software (Molecular Devices, LLC, Sunnyvale, CA, USA) to determine the Cy3 and Cy5 fluorescence intensity and background noise for all spots on the array. The microarray data were normalized to the median value, and log₂ ratios were calculated versus the values for the nontreated groups of the corresponding donor sample (n = 9). Genes that were up- or downregulated by more than threefold relative to the median value for all donor samples were further classified according to their protein types using the SOSUI program (http://bp.nuap.nagoya-u.ac.jp/sosui/).

Yamada T., Yoshii T., Yasuda H., Okawa A, & Sotome S. (2016). Dexamethasone Regulates EphA5, a Potential Inhibitory Factor with Osteogenic Capability of Human Bone Marrow Stromal Cells. Stem Cells International, 2016, 1301608.

+ Open protocol

+ Expand

Comparative Liver Transcriptome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA microarray analysis was performed on liver samples from a total of six mice (three mice each from the 10C and 6C + 4CP groups) by choosing average individuals from each group on the basis of their food efficiency values. For each sample, double-stranded complementary DNA (cDNA) was synthesised from 100 ng of purified total RNA, and biotinylated cRNA was transcribed from the cDNA using a GeneChip ® 3' IVT Express Kit (Affymetrix). The cRNA were subsequently fragmented and hybridised to a GeneChip ® Mouse Genome 430 2.0 Array (Affymetrix) containing over 34 000 mouse genes. The arrays were washed and labelled with streptavidin-phycoerythrin using a GeneChip ® Hybridization, Wash and Stain Kit and Fluidics Station 450 system (Affymetrix). Fluorescence was detected using a GeneChip ® Scanner 3000 7G (Affymetrix). All experimental procedures were carried out according to the manufacturer's instructions. All microarray data were Minimum Information About a Microarray Experiment (MIAME) compliant, and were deposited in a MIAME-compliant databasethe National Center for Biotechnology Information Gene Expression Omnibus (http:// www.ncbi.nlm.nih.gov/geo/; GEO Series accession number GSE75171)as detailed on The Functional Genomics Data Society website (http://fged.org/projects/miame/).

Tometsuka C., Koyama Y.I., Ishijima T., Toyoda T., Teranishi M., Takehana K., Abe K, & Nakai Y. (2017). Collagen peptide ingestion alters lipid metabolism-related gene expression and the unfolded protein response in mouse liver. The British journal of nutrition, 117(1).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!