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7100 electron microscope

Manufactured by Olympus
Sourced in Japan

The Olympus 7100 electron microscope is a high-performance instrument designed for advanced imaging and analysis. It utilizes an electron beam to generate detailed images of micro- and nano-scale structures, providing superior resolution and magnification capabilities compared to traditional optical microscopes.

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2 protocols using 7100 electron microscope

1

Electron Microscopy of Mouse Livers

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Mouse livers were fixed with a combination of paraformaldehyde and glutaraldehyde, and then livers were processed and sectioned with a diamond knife on copper grids after fixation. Grids were examined with a Hitachi (Tokyo, Japan) 7100 electron microscope, and images were captured using a MegaView III digital camera (Soft Imaging System, USA).
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2

Mitochondrial Morphology Analysis in Lung Cells

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SAEC and NHBE cells cultured in chamber slides or cover glass post CSE treatment were fixed with a combination of paraformaldehyde and glutaraldehyde (2%) fixative. Cells were then processed and sectioned with a diamond knife on copper grids. Grids were examined with a Hitachi (Tokyo, Japan) 7100 electron microscope and images were captured using a Megaview III digital camera (Soft Imaging System, Lakewood, CO) (Ahmad et al., 2015 (link)). Representative images were provided for mitochondrial localization and structural changes. Mitochondrial fragment length was analyzed manually from several images (1 μm magnification) that were captured from control and CSE treated SAEC and NHBE cells using ImageJ software.
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