Amylase assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), lipase assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), and C-reactive protein assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) were used to determine the activity of amylase (660 nm), lipase (420 nm), and C-reactive protein (570 nm), respectively, according to the instructions provided by the manufacturer.
Amylase assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Amylase Assay Kit is a laboratory equipment used to quantitatively measure the activity of the enzyme amylase in various biological samples. It provides a simple and reliable method for the determination of amylase levels.
Automatically generated - may contain errors
Lab products found in correlation
Lipase assay kit, by Nanjing Jiancheng (3 mentions)
Sybr premix ex taq kit, by Takara Bio (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Primescript rt master mix, by Takara Bio (1 mentions)
Caerulein cae, by Merck Group (1 mentions)
Inf39, by Selleck Chemicals (1 mentions)
Nfat5, by Santa Cruz Biotechnology (1 mentions)
Gapdh rabbit pab, by ABclonal (1 mentions)
Total protein quantitative assay kit, by Nanjing Jiancheng (1 mentions)
Trypsin assay kit, by Nanjing Jiancheng (1 mentions)
5 protocols using amylase assay kit
1
Enzymatic Assays for Amylase, Lipase, and CRP
2
Inflammatory Responses in Pancreatitis
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Caerulein (Cae) and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (USA). Enzyme-linked immunosorbent assay (ELISA) kits for IL-1β, IL-6, and TNF-α were purchased from BioLegend. The amylase assay kit was purchased from Jiancheng Biotechnology, Nanjing. Antibodies against NLRP3, myeloperoxidase (MPO) and β-actin were purchased from Cell Signaling Technology (CST; USA). TRIzol was purchased from Invitrogen (USA). PrimeScript RT Master Mix and SYBR Premix Ex Taq™ kit were purchased from Takara (Japan). INF-39 was purchased from Selleck Chemicals (USA).
3
Antibody Validation for Cellular Targets
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Antibodies against CDK5 (CAT#A23502), GAPDH Rabbit pAb (CAT#AC001), and Horseradish Peroxidase (HRP)-coupled Anti-Rabbit IgG Antibody (CAT#AS014) were purchased from ABclonal (Wuhan, China). NFAT5 (CAT#ER65748) was purchased from HUABIO (Zhejiang, China). GEF-H1 (CAT#AF4783) and Acetyl-alpha Tubulin (CAT#AF4351) were purchased from Affinity (Liyang, China). Antibodies against immunofluorescence CDK5 (CAT# 14145) and Acetyl-alpha-Tubulin (CAT#35652) were purchased from CST (USA); NFAT5 (CAT# sc-398171) was purchased from Santa Cruz Biotechnology (USA); rhodamine-labeled ghost pen cyclic peptides (TRITC Phalloidin, CAT# BL1189A) was purchased from Biosharp (Hefei, China). Rat tumor necrosis factor-α (TNF-α, Cat#F3768) and interleukin-6 (IL-6, Cat#F15870) were obtained from Xitang Biotechnology (Shanghai, China). The amylase assay kit (CAT#C016-1-1) was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
4
Enzymatic Activity in Freshwater Organisms
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Ten healthy individuals were randomly sampled from paddy fields and ponds, respectively. The number of individuals is 20 in this experiment. Intestine samples were washed with cold deionized water to remove most of the mucus, and the intestine was ground to pulp with cold sodium phosphate buffer (0.1 M, pH 7.0, 4 °C) by ratio of 1:9 (m/v). Then, the homogenate was centrifuged (3-18 K, Sigma®, Germany) at 4 °C at 3000×g for 10 min. The supernatant containing enzymes was stored at − 80 °C prior to the analysis. The lipase, amylase, trypsin and total protein were detected using assay Kits (Lipase assay kit, Amylase assay kit, Trypsin assay kit and Total protein quantitative assay kit) purchased from Nanjing Jiancheng, Bioengineering Institute, China. The specific procedures were reference kit’s instructions, and the protein concentration was used to normalize the enzymatic activity.
5
Serum Lipase and Amylase Assay
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The concentrations of lipase and amylase in serum were determined using Lipase Assay kit and Amylase Assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the instructions.
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